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Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages

  • Naoko Takeda-Okuda
  • Shuji Mizumoto
  • Zui Zhang
  • Soo-Ki Kim
  • Chi-Ho Lee
  • Byong-Tae Jeon
  • Yoshinao Z. Hosaka
  • Kenji Kadomatsu
  • Shuhei Yamada
  • Jun-ichi TamuraEmail author
Original Article
  • 31 Downloads

Abstract

Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed. GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate. Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations. High O- and N-sulfation degrees of HS correspond to high ossification. Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.

Keywords

Velvet antler Chondroitin sulfate Dermatan sulfate Heparan sulfate Keratan sulfate Hyaluronan 

Notes

Acknowledgements

We are grateful to Mr. Tsuyoshi Kawato for the kind gift of wild velvet antlers. We also thank Amano Enzyme Inc. (Nagoya, Japan) for kindly gifting PROTIN NY100. The authors thank Dr. Kenji Uchimura (UMR8576-CNRS, University of Lille), Ms. Kwon-Jung Yi (Konkuk University), Mr. Takamu Fujii, Ms. Yukino Ito (Meijo University), Mr. Yuga Inoue, Ms. Asumi Uemura, Mr. Daiki Sugita, Ms. Yuna Uemura, Ms. Yuka Omura, Ms. Akari Tani, and Ms. Marin Mitani (Tottori University) for their helpful assistance.

Author’s contribution

N.T.-O. performed the preparation of glycans and analyzed CS/DS and HA. S.M. and S.Y. performed HS analyses. Z.Z. and K.K. performed KS analyses. S.-K.K., C.-H.L., and B.-T.J. collected deer antlers. Y.Z.H. performed histological examinations. J.T. designed the study and wrote the manuscript.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Naoko Takeda-Okuda
    • 1
  • Shuji Mizumoto
    • 2
  • Zui Zhang
    • 3
  • Soo-Ki Kim
    • 4
  • Chi-Ho Lee
    • 4
  • Byong-Tae Jeon
    • 5
  • Yoshinao Z. Hosaka
    • 6
  • Kenji Kadomatsu
    • 3
  • Shuhei Yamada
    • 2
  • Jun-ichi Tamura
    • 1
    Email author
  1. 1.Department of Life and Environmental Agricultural Sciences, Faculty of AgricultureTottori UniversityTottoriJapan
  2. 2.Department of Pathobiochemistry, Faculty of PharmacyMeijo UniversityNagoyaJapan
  3. 3.Department of BiochemistryNagoya University Graduate School of MedicineNagoyaJapan
  4. 4.Department of Animal Science and Technology, College of Animal Bioscience and BiotechnologyKonkuk UniversitySeoulKorea
  5. 5.Korea Nokyong Research CenterKonkuk UniversityChungjuKorea
  6. 6.Veterinary Anatomy, Joint Department of Veterinary Medicine, Faculty of AgricultureTottori UniversityTottoriJapan

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