Glycoconjugate Journal

, Volume 35, Issue 6, pp 493–498 | Cite as

Separation of glycosphingolipids with titanium dioxide

  • Ayaka Noda
  • Miki Kato
  • Shota Miyazaki
  • Mamoru KyogashimaEmail author
Short Communication


We introduce the principle of a new technique to isolate glycosphingolipids (GSLs) from phospholipids. Neutral and acidic GSLs in organic solvent bind to titanium dioxide under neutral pH and can be eluted with 5 mg/ml of 2,5-dihydroxybenzoic acid in methanol. This special property is applicable for eliminating phospholipids, including sphingomyelin, which cannot be eliminated by a typical mild alkaline treatment. By using this technique, we demonstrated the rapid separation of minor components of GSLs, namely sulfatide and gangliosides from rabbit serum and liver, respectively. The minor GSL components were effectively purified despite both sources containing tremendous amount of phospholipids and simple lipids such as cholesterol, cholesteryl esters and triglycerides.


Glycosphingolipids Gangliosides Sulfatide Titanium dioxide Sphingomyelin 



We thank Ms. Miyagawa for assistance with GC-FID. We thank Renee Mosi, PhD, from Edanz Group ( for editing a draft of this manuscript.

Compliance with ethical standards

Conflicts of interest

This work was supported in part by G.L. Sciences Inc. Japan.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Ayaka Noda
    • 1
  • Miki Kato
    • 1
  • Shota Miyazaki
    • 2
  • Mamoru Kyogashima
    • 1
    Email author
  1. 1.Division of Microbiology and Molecular Cell BiologyNihon Pharmaceutical UniversitySaitamaJapan
  2. 2.GL Sciences Inc.SaitamaJapan

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