Nanoporous Glass with Controlled Pore Size for High-Efficiency Synthesis of Oligonucleotides
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The results of work on the development of powder nanoporous glass (NPG) with controlled pore size for solid-phase synthesis of oligonucleotides with heightened product yield are presented. NPG with a narrow pore-size distribution was obtained by varying the parameters of the heat and chemical treatment of sodium-borosilicate glasses. It was found that the functional charge of the material is equal to 72 μmol/g. It is shown that using the developed NPG in the synthesis of long-chain oligonucleotides increases the product yield more than seven-fold compared with the analogous material offered on the market.
Key wordsnanoporous glass alkali-borosilicate glass glass with controlled pore size CPG synthesis of oligonucleotides
This work was supported by the Ministry of Education and Science of the Russian Federation (contract No. 14.Z50.31.0009).
- 1.F. Rodrøguez-Reinoso and A. Sepèlveda-Escribano, Handbook of Surfaces and Interfaces of Materials, Elsevier, Amsterdam (2001).Google Scholar
- 5.M. M. Shults (ed.), Segregation Phenomena in Glasses [in Russian], Nauka, Leningrad (1974).Google Scholar
- 6.O. V. Mazurin, G. P. Roskova, V. I. Averyanov, and T. V. Antropova, Biphase Glasses: Structure, Properties, Applications [in Russian], Nauka, Leningrad (1991).Google Scholar
- 7.W. Haller, Porous Material and Method of Making the Same, Patent US3758284A (1973).Google Scholar
- 9.C. Mazilu, E. Rotiu, L. Ionescu, et al., “Nanoporous glass in Na2O–B2O3–SiO2 oxidic system, for potential biomedical applications,” J. Optoelectron. Adv. Mater., 9(7), 2036 – 2040 (2007).Google Scholar
- 10.R. T. Pon, Solid-Phase Supports for Oligonucleotide Synthesis, Current Protocols in Nucleic Acid Chemistry,Wiley, Chichester (2001).Google Scholar
- 13.N. Bian, S. Ramaswamy, N. Soice, et al., Media for Affinity Chromatography, Patent US772018B2 (2014).Google Scholar
- 15.CPG Product Info, LGC Biosearch Technologies; URL: https://www.biosearchtech.com/cpg (date of access: August 25, 2018).
- 16.Controlled Pore Glass (CPG), Sigma Aldrich URL: https://www.sigmaaldrich.com/chemistry/chemistryproducts. html?TablePage=16040627 (date of access: August 25, 2018).
- 17.O. V. Andreeva and I. E. Obyknovennaya, “Nanoporous matrices NPS-7 and NPS-17 — possibilities of use in an optical experiment,” Nanosistemy: Fiz., Khim., Matem., 1(1), 37 – 53 (2010).Google Scholar
- 18.R. T. Pon, “Solid-phase supports for oligonucleotide synthesis,” in: S. Agrawal (ed.), Protocols for Oligonucleotides and Analogs. Methods in Molecular Biology, Humana Press, NY(1993), Vol. 20.Google Scholar
- 19.B. I. Monogram, G. P. Roskova, and T. S. Tsekhomskaya, “Porous glasses: the process of formation, structure and some properties,” in: Physicochemistry of Silicates and Oxides [in Russian], Nauka, St. Petersburg (1998), pp. 199 – 216.Google Scholar
- 20.5′-DMT-T-Suc-CPG: 1000 Å, LGC Biosearch Technologies; URL: https://www.biosearchtech.com/products/synthesisreagents/cpg/5dmttsuccpg-1000-# (date of access, August 25, 2018).
- 21.M. A. Aleksashkina, B. I. Venzel, and L. G. Svatovskaya, “Porous glasses as a matrix for producing nanocomposites,” Fiz. Khim. Stekla, 31(3), 361 – 368 (2005).Google Scholar
- 23.M. A. Girsova, G. F. Golovina, L. N. Kurylenko, and T. V. Antropova, “Synthesis and study of bismuth-containing high-silica glass by the method of IR-spectroscopy,” Fiz. Khim. Stekla, 41(1), 127 – 132 (2015).Google Scholar