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Genetic variation and selection of MHC class I loci differ in two congeneric frogs

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Abstract

Major histocompatibility complex (MHC) genes encode proteins in the acquired immune response pathway that often show distinctive selection-driven patterns in wild vertebrate populations. We examined genetic variation and signatures of selection in the MHC class I alpha 1 (A1)- and alpha 2 (A2)-domain encoding exons of two frog congeners [Agalychnis callidryas (n = 20) and A. lemur (n = 20)] from a single locality in Panama. We also investigated how historical demographic processes may have impacted MHC genetic diversity by analyzing a neutral mitochondrial marker. We found that both MHC domains were highly variable in both species, with both species likely expressing three loci. Our analyses revealed different signatures of selection between the two species, most notably that the A. callidryas A2 domain had experienced positive selection while the A2 domain of A. lemur had not. Diversifying selection acted on the same number of A1 and A2 allelic lineages, but on a higher percentage of A1 sites compared to A2 sites. Neutrality tests of mitochondrial haplotypes predominately indicated that the two species were at genetic equilibrium when the samples were collected. In addition, two historical tests of demography indicated both species have had relatively stable population sizes over the past 100,000 years; thus large population size changes are unlikely to have greatly influenced MHC diversity in either species during this time period. In conclusion, our results suggest that the impact of selection on MHC diversity varied between these two closely related species, likely due to a combination of distinct ecological conditions and past pathogenic pressures.

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Acknowledgements

We thank Anna Savage, Jonathan Richmond and Steve Bogdanowicz for technical assistance, and Jeanne Robertson for specimen collection. This work was supported by the National Science Foundation (Grants DEB-0815315, DEB-0717741 and DEB-1120249 to K.Z. and K.L.) and a Cornell Center for Comparative Population Genomics Fellowship (to K.K.-T).

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  1. Karen M. Kiemnec-Tyburczy

    Present address: Department of Biological Sciences, Humboldt State University, Arcata, CA, 95521, USA

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  1. Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY, 14853, USA

    Karen M. Kiemnec-Tyburczy, Karen E. Tracy & Kelly R. Zamudio

  2. Department of Biology, University of Maryland, College Park, MD, 20742, USA

    Karen R. Lips

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  1. Karen M. Kiemnec-Tyburczy
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Correspondence to Karen M. Kiemnec-Tyburczy.

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Kiemnec-Tyburczy, K.M., Tracy, K.E., Lips, K.R. et al. Genetic variation and selection of MHC class I loci differ in two congeneric frogs. Genetica 146, 125–136 (2018). https://doi.org/10.1007/s10709-018-0016-0

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