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Insulin-like growth factor I of Yellow catfish (Pelteobagrus fulvidraco): cDNA characterization, tissue distribution, and expressions in response to starvation and refeeding

  • Qin Qin
  • Xiaohui Chen
  • Xiaochen Zhu
  • Xiangfei Li
  • Yingying Zhao
  • Zhiqiang Xu
  • Wenbin LiuEmail author
Article

Abstract

The full-length cDNA coding IGF-I was cloned from the liver of Yellow catfish Pelteobagrus fulvidraco. The tissue distributions of IGF-I in adults were then analyzed by using real-time PCR. The effects of starvation (3 weeks) and subsequent refeeding (3 weeks) on the compensatory growth performance in juvenile fish weighing 3.80 ± 0.78 g and hepatic IGF-I mRNA expressions were also investigated. The cDNA obtained covered 884 bp with an open reading frame of 480 bp encoding 159 amino acids. It is composed of a signal peptide with 41 amino acids (AAs), a mature peptide comprising the B, C, A, and D domains (71 AAs) and E domain of 47 AAs. Sequence alignment and phylogenetic analysis revealed a high degree of conservation (71–87%) among the species of Siluriformes and some closely related species. In adults, the highest IGF-I expression was observed in the liver, followed by the brain, whereas relatively low expressions were detected in muscle and stomach. Both body weight and length increased significantly in fish fed to satiation continuously. Body weight, body length, condition factor, and hepatic IGF-I expressions were all decreased remarkably with increasing starvation times, but increased significantly after refeeding. The results showed that the expression of IGF-I was positively correlated with feed intakes and IGF-I may play a key regulatory role for somatic growth induced by compensatory growth in Yellow catfish.

Keywords

Compensatory growth Insulin-like growth factor I Molecular cloning Pelteobagrus fulvidraco Tissue distribution and expressions 

Notes

Funding information

This study was funded by the Major Project for New Cultivar Breeding of Jiangsu Province (PZCZ201742) and the China Agriculture Research System (CARS-46). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable Institutional Animal Care and Use Committee (IACUC) of Nanjing Agricultural University were followed by the authors. All efforts were made to minimize the suffering of the animals.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Surgery of Traditional Chinese Medicine Research InstituteLonghua Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghaiChina
  2. 2.Freshwater Fisheries Research Institute of Jiangsu ProvinceNanjingChina
  3. 3.Liaoning Provincial key Laboratory of Zoonosis, College of Animal Science & Veterinary MedicineShenyang Agricultural UniversityShenyangChina
  4. 4.Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and TechnologyNanjing Agricultural UniversityNanjingChina

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