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Fish Physiology and Biochemistry

, Volume 45, Issue 1, pp 231–243 | Cite as

The accumulation, histopathology, and intestinal microorganism effects of waterborne cadmium on Carassius auratus gibelio

  • Jun Liu
  • Juan-juan Pang
  • Zong-cai TuEmail author
  • Hui Wang
  • Xiao-mei ShaEmail author
  • Yan-hong Shao
  • Guang-xian Liu
Article

Abstract

Cadmium (Cd) is known to be a potentially toxic heavy metals to the fish health and growth. Carassius auratus gibelio (C. a. gibelio) specimens were exposed to waterborne Cd (0, 0.05, 0.10, 0.15, and 0.20 mg/L CdCl2) for 14 days. Cd accumulation, liver and intestine histopathology, and intestinal microorganism were investigated in the present study. The results indicated that Cd accumulation in the gill, liver, intestine, and muscle gradually decreased as Cd concentration increased. The gill accumulated higher amounts of Cd than other tissues. The histopathology of liver and intestine underwent changes with different Cd concentrations, including hepatocyte hypertrophy, aggregation of blood cells, sinusoids, lipidosis, necrosis of hepatic tissues, the erosion of villi, necrosis in the mucosal layer, the appearance of vacuoles in the lamina propria, hyperplasia, and swelling of goblet cells. Moreover, the core gut microbiota existed in the intestinal microorganism and did not change as Cd concentration increased. However, the diversity of intestinal microorganism was significantly reduced compared with that of the control sample. The present results indicated that C. a. gibelio exposed to Cd suffered toxicity, and Cd could affect the biodiversity of the intestinal microbiota of C. a. gibelio.

Keywords

Cadmium Freshwater fish Toxicity Bioaccumulation Gut microbiota 

Notes

Funding information

This work was supported by the earmarked fund for China Agriculture Research System (CARS-45), Excellent Youth Foundation of Jiangxi Province (20162BCB23017), National Science Foundation of Jiangxi Province (20142BAB213016), Science and Technology Support Program of Jiangxi Province (2014BBF60043), and Innovation Foundation of Nanchang University (cx2015110).

Compliance with ethical standards

All experimental procedures used in the present study were approved by the Animal Care Review Committee, Nanchang University.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.College of Life ScienceJiangxi Normal UniversityNanchangChina
  2. 2.State Key Laboratory of Food Science and TechnologyNanchang UniversityNanchangChina

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