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Fish Physiology and Biochemistry

, Volume 45, Issue 1, pp 105–114 | Cite as

Evaluation of seminal plasma composition and spermatozoa quality parameters of silver barb, Barbonymus gonionotus Bleeker, 1850

  • Ibrahim Rashid
  • Md Shakhawate HossainEmail author
  • Mohammad Abdus Salam
  • S. M. Rafiquzzaman
Article

Abstract

Seminal composition and semen quality are the important determinants in assessing the reproductive performance of different fishes. This study was carried out to evaluate the seminal composition and sperm quality of Barbonymus gonionotus. The seminal plasma contained 17.2 ± 0.34 mmol/l, 20.9 ± 0.48 mmol/l, 0.72 ± 0.04 mmol/l, 3.8 ± 0.2 mmol/l, and 1.49 ± 0.02 g/dl of Na+, K+, Ca++, Mg++, and total protein, respectively. The physical spermatological parameters, such as sperm volume, sperm motility, motility duration, sperm density, osmolality, and pH values were 1.55 ± 0.15 ml, 89 ± 2%, 391.9 ± 8.5 s, 2.8 ± 0.2 × 1010 /ml, 400.6 ± 5.1 mmol/kg, and 8.75 ± 0.10, respectively. In correlation matrix, the K+ (R2 = 0.39, P < 0.01) and Ca++ (R2 = 0.27, P < 0.05) ions and osmolality (R2 = 0.29, P < 0.05) showed significant positive correlations with sperm motility. Similarly, fertilization rate significantly influenced by sperm motility (R2 = 0.26, P < 0.05) and K+ (R2 = 0.30, P < 0.05) and Ca++ (R2 = 0.26, P < 0.05) ions. Also, osmolality significantly and negatively correlated with Mg++ (R2 = 0.33, P < 0.05) and sperm motility duration (R2 = 0.28, P < 0.05). Therefore, based on this results, it can be concluded that seminal plasma ions, K+ and Ca++ and osmolality are the key factors for the determination of sperm quality of silver barb, and these parameters could be considered during standardization of artificial fertilization or cryopreservation technique of silver barb spermatozoa.

Keywords

Spermatozoa motility Plasma ion Osmolality Barbonymus gonionotus Silver barb 

Notes

Acknowledgements

Authors would like to thank to Prof. Dr. GKM Mustafizur Rahman for his kind help during sample analysis and to Dr. Hamid Niksirat for his valuable guidelines during manuscript preparation.

Funding information

This study is supported by the Research Management Committee (RMC) of Bangabandhu Sheikh Mujibur Rahaman Agricultural University (Grant No. UGC/RMC-55, Section-8) and the Ministry of Education, Youth and Sports of the Czech Republic—projects “CENAKVA” (No. CZ.1.05/2.1.00/01.0024), “CENAKVA II” (No. LO1205 under the NPU I program), and by the Grant Agency of University of South Bohemia (No. 017/2016/Z).

Supplementary material

10695_2018_539_MOESM1_ESM.docx (38 kb)
Table S1 (DOCX 38 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Fisheries Biology and Aquatic EnvironmentBangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipurBangladesh
  2. 2.Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture and Biodiversity of HydrocenosesUniversity of South Bohemia in České BudějoviceVodňanyCzech Republic
  3. 3.Department of Genetics and Fish BreedingBangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipurBangladesh

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