DNA content of hepatocyte and erythrocyte nuclei of the spined loach (Cobitis taenia L.) and its polyploid forms
- 165 Downloads
We analyzed the DNA content of hepatocyte and erythrocyte nuclei of the spined loach Cobitis taenia (diploid) and its allopolyploid forms. Twenty triploid females and one tetraploid were used. At least 20,000 hepatocyte and erythrocyte nuclei were acquired and analyzed by flow cytometry. C. taenia erythrocyte nuclei contain 3.15 ± 0.21 pg of DNA and the hepatocyte nuclei 4.45 ± 0.46 pg of DNA. Triploid Cobitis have 5.08 ± 0.41 pg of DNA in erythrocyte nuclei and 6.11 ± 0.40 pg of DNA in hepatocyte nuclei, whereas the tetraploid erythrocyte and hepatocyte nuclei contained 6.60 and 7.40 pg of DNA, respectively. In general, the DNA contents correlate positively with the ploidy level of the fish investigated. The DNA content variation in the hepatocyte and erythrocyte nuclei may be due to differences in extent of chromatin condensation, which is more pronounced in the erythrocyte than hepatocyte nuclei, or to the several orders of ploidy that occur in the parenchymal liver cells.
KeywordsCobitis DNA Polyploidy Spined loach
We thank Professor Elżbieta Pyza chief of the Department for providing facility used during this study and hospitality for one of us (W·K.), Dr. Gregory Tylko who introduced me (W·K.) to the techniques of Flow Cytometry. We would like to thank N.J. Severs for critical comments on the manuscript.
- Boron A (1994) Use of erythrocyte measurements to detect natural triploids of spined loach Cobitis taenia (L.). Cytobios 78:197–202Google Scholar
- Boron A (2003) Karyotypes and cytogenetic diversity of the genus Cobitis (Pisces, Cobitidae) in Poland: a review. Cytogenetic evidence for a hybrid origin of some Cobitis triploids. Folia Biol (Krakow) 51:50–54Google Scholar
- Fenerich PC, Foresti F, Oliveira C (2004) Nuclear DNA content in 20 species of Siluriformes (Teleostei; Ostariophysi) from the Neotropical region. Genet Mol Biol 21:47–54Google Scholar
- Gao Z, Wang W, Abbas K, Zhou X, Yang Y, Diana JS, Wang H, Wang H, Li Y, Sun Y (2007) Haematological characterization of loach Misgurnus anguillicaudatus: comparison among diploid, triploid and tetraploid specimens. Fish Physiol Biochem 147:1001–1008Google Scholar
- Gregory TR (2000) Animal genome size database. http://www.genomesize.com
- Lamatsch DK, Steinlein C, Schmid M, Schartl M (2000) Non-invasive determination of genome size and ploidy level in fishes by flow cytometry: detection of triploid Poecillia formosa. Cytometry 39:91–95. doi: 10.1002/(SICI)1097-0320(20000201)39:2<91::AID-CYTO1>3.0.CO;2-4 CrossRefGoogle Scholar
- Vasil’ev VP, Vinogradov AE, Rozanov YuM, VasilIeva ED (1999) Cellular DNA content in different forms of the bisexual-unisexual complex of spined loaches of the genus Cobitis and in luther’s spined loach C. lutheri (Cobitidae). J Ichthyol 39:377–383 (in Russian)Google Scholar
- Vinogradov AE (1998) Genome size and GC-percent in vertebrates as determined by flow cytometry: the triangular relationship. Cytometry 31:100–109. doi: 10.1002/(SICI)1097-0320(19980201)31:2<100::AID-CYTO5>3.0.CO;2-Q CrossRefGoogle Scholar