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Euphytica

, 215:168 | Cite as

Identification of SNP markers linked to the weeping trait in Prunus mume

  • Suzhen Li
  • Tangchun Zheng
  • Xiaokang Zhuo
  • Lulu Li
  • Like Qiu
  • Jia Wang
  • Tangren Cheng
  • Qixiang ZhangEmail author
Article
  • 27 Downloads

Abstract

Prunus mume is a traditional ornamental tree with a graceful architecture that blossoms early in spring and presents beautiful flowers with a pleasant fragrance. Recently, the weeping trait has received increasing attention for its ornamental appeal and potential application to agriculture. In this study, we identified the SNPs tightly linked to the weeping trait using a linkage population based on specific-locus amplified fragment sequencing (SLAF-Seq). Six SLAF-derived SNP markers (Marker446598, Marker353041, Marker315769, Marker334902, Marker301243 and Marker311414) were validated as being tightly associated with the weeping phenotype using Sanger sequencing. The Sanger sequencing results of Marker353041 indicated that 100% of the weeping individuals and 73.3% of the upright individuals were homozygous (AA) and heterozygous (AT), respectively. Two genotypes were identified using Marker301243 with allele-specific polymerase chain reaction. All upright individuals were heterozygous (TA) and only 16.7% of the weeping individuals heterozygous (TA) in the F1 segregated population. Two marker combinations led to 89.13% predictability in the cultivars. The results suggest the application of this approach for marker-assisted breeding of P. mume and lay the foundation for the molecular breeding process of the weeping trait in woody ornamental plants.

Keywords

Prunus mume Weeping trait SNP marker Genotyping Ornamental plant 

Notes

Acknowledgements

The research was supported by the program for Science and Technology of Beijing (No. Z181100002418006) and Special Fund for Beijing Common Construction Project.

Author contributions

SL and TZ conceived and drafted the manuscript. TZ conceived and designed the experiments. SL and XZ performed the experiments. LL and LQ analysed the data. JW and TC contributed reagents/materials/analysis tools. QZ contributed to the conception of the study and finalized the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest and this research has been conducted in the absence of any financial or commercial relationships.

Supplementary material

10681_2019_2488_MOESM1_ESM.jpg (802 kb)
Supplementary Figure 1: Heatmap showing the correlation between the markers. (JPG 801 kb)
10681_2019_2488_MOESM2_ESM.jpg (1.3 mb)
Supplementary Figure 2: AS-PCR amplification results of Marker301243 and Marker311414 in the F1 population. 1–6: upright type of F1 population; 7–12: weeping type of F1 population; and M: Marker DL2000. (JPG 1294 kb)
10681_2019_2488_MOESM3_ESM.jpg (433 kb)
Supplementary Figure 3: AS-PCR amplified results of Marker301243 and Marker311414 in cultivars. (JPG 433 kb)
10681_2019_2488_MOESM4_ESM.jpg (375 kb)
Supplementary Figure 4: Accuracy of Marker 301243 and Marker 311414 in the F1 population and cultivars. a Accuracy of Marker 301243 and Marker 311414 for molecular-assisted selection in the F1 population; b accuracy of Marker 301243 and Marker 311414 for molecular-assisted selection in cultivars; c accuracy of marker combination (Marker 301243 and Marker 311414) for molecular-assisted selection in the F1 population and cultivars. (JPG 375 kb)
10681_2019_2488_MOESM5_ESM.docx (20 kb)
Supplementary File 1: Seventeen SLAF marker sequences. (DOCX 19 kb)
10681_2019_2488_MOESM6_ESM.docx (15 kb)
Supplementary File 2: Primers used for Sanger sequencing and AS-PCR. (DOCX 15 kb)
10681_2019_2488_MOESM7_ESM.xlsx (10 kb)
Supplementary Table 1: Information of 13 markers identified by Sanger sequencing. (XLSX 9 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape ArchitectureBeijing Forestry UniversityBeijingPeople’s Republic of China
  2. 2.Beijing Advanced Innovation Center for Tree Breeding By Molecular DesignBeijing Forestry UniversityBeijingPeople’s Republic of China

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