, 215:159 | Cite as

Developing genetically segregating populations for localization of novel sugarcane brown rust resistance genes

  • Xiao-Yan Wang
  • Wen-Feng Li
  • Ying-Kun HuangEmail author
  • Hong-Li Shan
  • Rong-Yue Zhang
  • Jie Li
  • Xiao-Yan Cang
  • Zhi-Ming Luo
  • Jiong Yin


Brown rust caused by Puccinia melanocephala H. Sydow & P. Sydo is an important sugarcane disease that severely threatens sugarcane production in China. Breeding and planting resistant cultivars is the most cost-effective and efficient strategy for the control the disease. In addition, exploration, research, and utilization of resistant genes are the basis for resistance breeding. In the present study, four sugarcane varieties without the Bru1 gene and highly resistant to brown rust were used as male parents, and four varieties highly susceptible to brown rust were used as the female parents to configure hybrids. Six F1 hybrid populations were subjected to authenticity determination using SSR markers, brown rust resistance phenotype identification using artificial inoculation, and molecular detection of Bru1 gene. The results showed that the genetic segregation ratios in F1 hybrid populations of Yuetang 03-393 (HS) × ROC24 (HR) and Liucheng 03-1137 (HS) × Dezhe 93-88 (HR) were 3R:1S and 1R:3S, respectively, and Bru1 gene was not detected in their F1 hybrids. The results indicated that two dominant novel genes controlled brown rust resistance in Yuetang 03-393 (HS) × ROC24 (HR) and two recessive novel genes controlled brown rust resistance in Liucheng 03-1137 (HS) × Dezhe 93-88 (HR). Two genetically segregating populations that could be used for the localization of novel brown rust resistance genes were developed in the present study, and could lay a suitable foundation and provide theoretical reference for the analysis of the genetic mechanism of brown rust resistance, genetic map construction, mapping of novel resistance genes and the development of closely associated molecular markers in the future.


Sugarcane Brown rust resistance Novel gene Genetically segregating populations 



This work was supported by the Natural Science Foundation of China (31660419), Sugar Crop Research System (CARS-170303), Yunling industry and technology leading talent training program "Prevention and Control of Sugarcane Pests" (2018LJRC56) and Yunnan Province Agriculture Research System.


  1. Aitken KS, Jackson PA, Mcintyre CL (2005) A combination of AFLP and SSR markers provides extensive map coverage and identification of homo(eo)logous linkage groups in a sugarcane cultivar. Theor Appl Genet 110(5):789–801CrossRefGoogle Scholar
  2. Asnaghi C, D’hont A, Glaszmann J, Rott P, (2001) Resistance of sugarcane cultivar R 570 to Puccinia melanocephala isolates from different geographic locations. Plant Dis 85:282–286CrossRefGoogle Scholar
  3. Asnaghi C, Roques D, Ruffel S, Kaye C, Hoarau JY, Télismart H, Girard JC, Roboin LM, Risterucci AM, Grivet L, D’Hont A (2004) Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers. Theor Appl Genet 108:759–764CrossRefGoogle Scholar
  4. Butterfield MK (2007) Marker assisted breeding in sugarcane: a complex polyploid. University of Stellenbosch, StellenboschGoogle Scholar
  5. Chen Q L, 1982. Sugarcane disease in the World. Beijing: Agriculture Press, 1982, pp 34–40 .Google Scholar
  6. Comstook JC (1992) Effect of rust on sugarcane growth and biomass. Plant Dis 76(2):172–177Google Scholar
  7. Comstook JC, Shine JM, Raid RN (1992) Effece of early rust infection on subsequent sugarcane growth. Sugar Cane 4:7–9Google Scholar
  8. Comstock JC, Wu KK, Schnell RJ (1992) Heritability of resistance to sugar cane rust. Sugar Cane (United Kingdom) 6:7–10Google Scholar
  9. Cordeiro GM, Taylor GO, Henry RJ (2000) Characterisation of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploid species. Plant Sci 155:161–168CrossRefGoogle Scholar
  10. Costet L, Toubi L, Le Cunff L (2012) Haplotype structure around Bru1 reveals a narrow genetic basis for brown rust resistance in modern sugarcane cultivars. Theor Appl Genet 125:825–836CrossRefGoogle Scholar
  11. CSIRO (2005) Unlocking success through change and innovation: Options to improve the profitability and environmental performance of the Australian sugar industry. Submission to Sugarcane Industry Assessment, pp 4.Google Scholar
  12. Daugrois JH, Grivet L, Roques D (1996) A putative major gene for rust resistance linked with a RFLP marker in sugarcane cultivar‘R570’. Theor Appl Genet 92:1059–1064CrossRefGoogle Scholar
  13. Fang XJ, Wu WR, Tang JL (2002) DNA marker assisted breeding in crops. Science Press, BeijingGoogle Scholar
  14. Glynn NC, Laborde C, Davidson RW, Irey MS, Glaz B, D'Hont A, Comstock JC (2013) Utilization of a major brown rust resistance gene in sugarcane breeding. Mol Breed 31:323–331CrossRefGoogle Scholar
  15. Grivet L, Arruda P (2002) Sugarcane genomics: depicting the complex genome of an important tropical crop. Curr Opin Plant Biol 5:122–127CrossRefGoogle Scholar
  16. Hoarau J Y, Souza G, D'Hont A, Menossi M, Pinto L R, de Souza A P, Grivet L, Menck C M, Ulian E C, Vincentz M. 2007. Sugarcane, a tropical crop with a highly complex genome. Funct Plant Genom 481–499.Google Scholar
  17. Hogarth DM, Ryan CC, Taylor PWJ (1993) Quantitative inheritance of rust resistance in sugarcane. Field Crop Res 34:187–193CrossRefGoogle Scholar
  18. Hoy JW, Hollier CA (2009) Effect of brown rust on yield of sugarcane in Louisiana. Plant Dis 3:1171–1174CrossRefGoogle Scholar
  19. Huang YK, Li WF (2011) Colored Atlas of main diseases, insect pests and weeds of modern sugarcane. China Agriculture Press, Beijing, pp 104–106 (in Chinese)Google Scholar
  20. Huang YK, Li WF (1998) Epidemic and control strategies of sugarcane rust disease in Yunnan sugarcane field. Plant Prot Technol Extens 18:22–23Google Scholar
  21. Josefina R, Marıa FP, Romina B, Claudia F, Victoria G, Marıa IC, D’Hont Angelique, Bjorn W, Atilio PC (2013) Bru1 gene and potential alternative sources of résistance to sugarcane brown rust disease. Euphytica 191:429–436CrossRefGoogle Scholar
  22. Le Cunff L, Garsmeur O, Raboin LM, Pauquet J, Telismart H, Selvi A, Grivet L, Philippe R, Begum D, Deu M, Costet L, Wing R, Glaszmann JC, D’Hont A (2008) Diploid/polyploid syntenic shuttle mapping and haplotype-specific chromosome walking toward a rust resistance gene (Bru1) in highly polyploid sugarcane (2n ∼ 12x ∼ 115). Genetics 180:649–660CrossRefGoogle Scholar
  23. Li WF, Huang YK (2012) Diagnostic testing and control technology for modern sugarcane diseases. China Agriculture Press, Beijing, p 94Google Scholar
  24. Li WF, Wang XY, Huang YK, Zhang RY, Shan HL, Yin J, Luo ZM (2015) Identification of resistance to brown rust and molecular detectin of Bru1 gene in 31 wild core sugarcane germplasms. Acta Agron Sin 41(5):806–812CrossRefGoogle Scholar
  25. Li WF, Wang XY, Huang YK, Zhang RY, Shan HL, Yin J, Luo ZM (2016) Molecular detection of Bru1 gene and identification of brown rust resistance in Chinese sugarcane germplasm. Sugar Tech 19(2):1–8Google Scholar
  26. Li WF, Shan HL, Zhang RY, Pu HC, Wang XY, Cang XY, Yin J, Luo ZM, Huang YK (2018) Identification of field resistance and molecular detection of the brown rust resistance gene Bru1 in new elite sugarcane varieties in China. Crop Prot 103:46–50CrossRefGoogle Scholar
  27. Liu XM, Liu WB, Shi HH (2008) Pathogen identification and biological characteristics of sugarcane rust in Danzhou. Sugar Crops China 2:30–32Google Scholar
  28. Liu XL, Cai Q, Bi Y, Lu X, Ma L, Ying XM, Mao J (2009) A rapid silver staining method for PAGE used in sugarcane AFLP and SSR molecular markers. Jiangsu J Agric Sci 25(2):433–435Google Scholar
  29. Liu XL, Ma L, Chen XK, Ying XM, Cai Q, Liu JY, Wu CW (2010) Establishment of DNA fingerprint ID in sugarcane cultivars in Yunnan. China Acta Agron Sin 36(2):202–210Google Scholar
  30. Ma L (1995) The production and research actuality of sugarcane in Thailand. World Agric 9:23Google Scholar
  31. Molina L, Queme JL, Rosales F (2013) Comparative analysis between phenotype and Bru1 marker for incidence to brown rust in sugarcane. Proc Int Soc Sugar Cane Technol 28:1–6Google Scholar
  32. Pan YB (2006) Highly Polymorphic Microsatellite DNA Markers for Sugarcane Germplasm Evaluation and Variety Identity Testing. Sugar Tech 8(4):246–256CrossRefGoogle Scholar
  33. Pinto LR, Oliveira KM, Ulian EC, Garcia AA, de Souza AP (2004) Survey in the sugarcane expressed sequence tag database (SUCEST) for simple sequence repeats. Genome 47:795–804CrossRefGoogle Scholar
  34. Raboin LM, Oliveira KM, Lecunff L, Telismart H, Roques D, Butterfield M, Hoarau JY, Hont AD (2006) Genetic mapping in sugarcane, a high polyploid, using bi-parental progeny: identification of a gene controlling stalk colour and a new rust resistance gene. Theor Appl Genet 112:1382–1391CrossRefGoogle Scholar
  35. Raid RN, Comstock JC (2000) Common rust. In: Rott P, Bailey RA, Comstock JC, Croft BJ, Saumtally AS (eds) A guide to sugarcane diseases. CIRAD and ISSCT, Montpellier, pp 85–89Google Scholar
  36. Raid R H, 2006. Florida Sugarcane Handbook. University of Florida, pp 1–3.Google Scholar
  37. Ramdoyal K, Sullivan S, Chong LCYLS, Badaloo GH, Saumtally S, Domaingue R (2000) The genetics of rust resistance in sugar cane seedling populations. Theor Appl Genet 100:557–563Google Scholar
  38. Ruan XY, Yan F, Sun CJ (1983) Occurrence of Puccinia erianthi on sugarcane in Yunnan province. Acta Mycologica Sin 2:260–261Google Scholar
  39. Santos FRC, Pinto LR, Carlini-Garcia LA, Gazaffi R, Mancini MC, Gonçalves BS, Medeiros CNF, Perecin D, Garcia AAF, Souza AP, Zucchi MI (2015) Marker-trait association and epistasis for brown rust resistance in sugarcane. Euphytica 203:533–547CrossRefGoogle Scholar
  40. The important sugarcane diseases research cooperation group (1991) The preliminary report of sugarcane diseases investigation in the sugarcane planting provinces (partly), Mainland China. Sugarcane Canrsugar 1:1–8Google Scholar
  41. Wang JP, Roe B, Macmi S, Yu QY, Murray JE, Tang HB, Chen CX, Najar F, Wiley G, Bowers J, Sluys MAV, Rokhsar DS, Hudson ME, Moose SP, Paterson AH, Ming R (2010) Microcollinearity between autopolyploid sugarcane and diploid sorghum genomes. BMC Genom 11(261):1–17Google Scholar
  42. Wei JJ, Deng ZY, Huang WH (2010) Control methods and pathogen biological characteristics of sugarcane rust in Beihai. Anhui Agric Sci 38:14997–14999Google Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Sugarcane Research Institute, Yunnan Key Laboratory of Sugarcane Genetic ImprovementYunnan Academy of Agricultural ScienceKaiyuanChina

Personalised recommendations