, 215:152 | Cite as

Identification and physical mapping of QTLs associated with flowering time in Brassica napus L.

  • Kunjiang Yu
  • Xiaodong Wang
  • Wenjing Li
  • Lijie Sun
  • Qi Peng
  • Feng Chen
  • Wei Zhang
  • Rongzhan Guan
  • Jiefu ZhangEmail author


Flowering time regulation is crucial to yield of rapeseed (Brassica napus L.). However, insight into the loci controlling flowering time variation in rapeseed is not clear enough. In this study, a recombinant inbred line population was used for quantitative trait locus (QTL) analysis associated with flowering time in five different environments. A total of 36 identified QTLs were obtained, and each explained 3.96–24.41% of the phenotypic variation (PV). The QTL meta-analysis showed that 36 identified QTLs were integrated into 24 consensus QTLs. Of these, eight consensus QTLs were stably expressed in at least two environments, and four with PV ≥ 10% were specifically expressed in a single environment—these 12 consensus QTLs were considered the key QTLs controlling flowering time in the AH population. Physical mapping indicated that the 12 key QTLs were mapped into 12 single genomic fragments in B. rapa and B. oleracea, and physical positions of these QTLs in the B. napus genome were further determined by referring to the B. rapa and B. oleracea genomes. Ultimately, seven candidate genes underlying the confidence intervals of five key QTLs were identified. These findings will contribute to further insight into the genetic architecture of flowering time in rapeseed.


Brassica napus L. Flowering time QTL Physical mapping 



The work was supported by National Key Research and Development Program of China (2018YFD0100601), National Natural Science Foundation of China (31601334), Earmarked fund for China Agriculture Research System (CARS-12), Natural Science Foundation of Jiangsu Province (BK20160578), and Jiangsu Collaborative Innovation Center for Modern Crop Production. The funding bodies had no role in the experiment design, data analysis, preparation of the manuscript and decision to publish. We thank International Science Editing ( for editing this manuscript.

Supplementary material

10681_2019_2480_MOESM1_ESM.tif (18.2 mb)
Supplementary Figure 1. Locations of flowering-time QTLs on chromosomes A2, A4, A7, A10, C2, C3, C6 and C7. For simplicity, only markers in the QTLs’ CIs and the two terminal markers of each linkage group are shown. The definitions of the colorized bars and markers are same as in Figure 2 (TIFF 18619 kb)
10681_2019_2480_MOESM2_ESM.xlsx (9 kb)
Supplementary material 2 (XLSX 9 kb)
10681_2019_2480_MOESM3_ESM.xlsx (25 kb)
Supplementary material 3 (XLSX 24 kb)
10681_2019_2480_MOESM4_ESM.docx (44 kb)
Supplementary material 4 (DOCX 44 kb)
10681_2019_2480_MOESM5_ESM.xlsx (10 kb)
Supplementary material 5 (XLSX 9 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Kunjiang Yu
    • 1
    • 2
  • Xiaodong Wang
    • 1
  • Wenjing Li
    • 3
  • Lijie Sun
    • 1
  • Qi Peng
    • 1
  • Feng Chen
    • 1
  • Wei Zhang
    • 1
  • Rongzhan Guan
    • 4
  • Jiefu Zhang
    • 1
    Email author
  1. 1.Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Institute of Industrial CropsJiangsu Academy of Agricultural SciencesNanjingChina
  2. 2.College of AgricultureGuizhou UniversityGuiyangChina
  3. 3.Jiangsu Institute of Science and Technology InformationNanjingChina
  4. 4.State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina

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