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Euphytica

, 215:169 | Cite as

New melon introgression lines in a Piel de Sapo genetic background with desirable agronomical traits from dudaim melons

  • Gabriel Castro
  • Gorka Perpiñá
  • Antonio José Monforte
  • Belén PicóEmail author
  • Cristina EsterasEmail author
Article

Abstract

A new collection of introgression lines (ILs) have been developed using the Spanish cultivar ‘Piel de Sapo’ (PS, subsp. melo, group ibericus) as the recurrent parent, and ‘Queen’s pocket melon’ (DUD, subsp. melo, group dudaim PI 273438) as donor. Genome-wide markers assisted selection has been proceed in several backcross generations to obtain a set of 16 ILs. An average of 1.4 introgressions/plant representing altogether about 62% of the DUD genome, and with an average of recovery per IL of 93.9% of the PS genetic background, has been accomplished in the selected ILs after several backcrosses and selfings. QTLs for abscission layer, external aroma, rind and flesh firmness, rind netting, fruit weight and shape, and sugar content have been identified on this set of ILs some of them corresponding to genomic regions previously described or with interesting candidate genes, but, also, new allelic diversity has been identified. Thus, this set of ILs may be useful to exploit new underexploited genetic variability from DUD. Based on GBS results for five of this ILs, further steps of backcrossing will be necessary to clean up the small introgressions detected in some of them. Novel phenotypes like small PS melons or aromatic and medium-climacteric PS melons were obtained, which might become pre-breeding lines with potential commercial interest.

Keywords

C. melo Genetic resources QTLs Climacteric Piel de Sapo Small melons Quality traits 

Notes

Acknowledgements

This work was supported by the Spanish Ministerio de Educación through an ERA-NET Plant Genomics project (MELRIP: GEN2006-27773-C2-2-E), by the Spanish Ministerio de Economía de Empresa through a Plant KBBE Project (SAFQIM: PIM2010PKB-00691), by the Spanish Ministerio de Economía y Competitividad AGL2015-64625-C2-2-R, and by the Spanish Ministerio de Ciencia, Innovación y Universidades AGL2017-85563-C2-1-R (jointly funded by FEDER). This work was also partly supported by the Programa de Valorización y Recursos Conjuntos de I + D + i de VLC/CAMPUS funded by the Ministerio de Educación, Cultura y Deporte as part of the Programa Campus de Excelencia Internacional.

Supplementary material

10681_2019_2479_MOESM1_ESM.xlsx (32 kb)
Online resource 1. SNPs employed in the construction of the IL population. This set was selected from those previously mapped and employed to anchor the first version of the melon genome (Garcia-Mas et al. 2012). Also, positions in the new genetic map by Argyris et al. (2015) are indicated, as well as the physical position for genome assembly in the version v.3.5.1 and the most recent v.3.6.1, available at Melonomics. Information about the SNP and flanking sequence is included for all the markers used in the Agena Bioscience assay, and the primers for those that were adapted to the HRM genotyping procedure are also provided. Markers that could not be accurately called in the Agena Bioscience assay are marked as failed markers (f) (XLSX 31 kb)
10681_2019_2479_MOESM2_ESM.xlsx (17 kb)
Online resource 2. Evaluation of the normality of every trait distribution for Paip16 and Paip17 populations with the Shapiro–Wilk and Kolmogorov–Smirnov tests using Statgraphics Centurion XVII.II (XLSX 17 kb)
10681_2019_2479_MOESM3_ESM.xlsx (24 kb)
Online resource 3. Graphical genotype of the 16 ILs selected. Location in chromosomes including genetic position according to maps by Garcia-Mas et al. (2012) and Argyris et al. (2015), and physical position in melon genome assembly v.3.6.1, for the genetic background markers is indicated. Violet boxes indicate DUD homozygous introgressions, green boxes indicate the PS genetic background, yellow boxes indicate DUD heterozygosis and red boxes correspond to no calls (XLSX 23 kb)
10681_2019_2479_MOESM4_ESM.xlsx (526 kb)
Online resource 4. GBS results. A. Genotype data for the 5 selected ILs and parents PS and DUD genotyped by GBS. B. Summary of introgressions found in the 5 selected ILs genotyped using GBS technology (only introgressions longer than 5Kbp) (XLSX 525 kb)
10681_2019_2479_MOESM5_ESM.xlsx (30 kb)
Online resource 5. Mean value, standard deviation and standard error of the mean, along with ANOVA results for means comparison of both parents, PS and DUD, is shown for each trait in the two trials in which they were phenotyped along with the ILs. The asterisk (*) indicate significant difference with respect to the parent PS (XLSX 29 kb)
10681_2019_2479_MOESM6_ESM.xlsx (18 kb)
Online resource 6. QTLs identified by the Dunnet’s Test in two environments in the IL collection. Abbreviated trait name, QTL name, chromosome, QTL position (cM) and flanking markers, DUD effect relative to the PS parental abçnd expressed in percentage (%) with positive/negative effects, IL carrying the introgression. Introgression position (cM) and flanking markers in the IL, and information about other introgressions in these ILs (XLSX 17 kb)
10681_2019_2479_MOESM7_ESM.xlsx (13 kb)
Online resource 7. Percentage of variance explained for every trait for genotype, environment and the interaction (XLSX 12 kb)
10681_2019_2479_MOESM8_ESM.xlsx (24 kb)
Online resource 8. Genes located in the introgressions detected in the set of 16 ILs related to the traits analyzed (XLSX 23 kb)
10681_2019_2479_MOESM9_ESM.xlsx (14 kb)
Online resource 9. Correlation coefficient (p < 0.05) for the traits analyzed in both trials Paip16 and Paip17 (XLSX 14 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Instituto de Conservación y Mejora de la Agrodiversidad ValencianaUniversitat Politècnica de València (COMAV-UPV)ValenciaSpain
  2. 2.Instituto de Biología Molecular y Celular de Plantas (IBMCP) UPV-CSICValenciaSpain

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