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Euphytica

, 215:147 | Cite as

QTL mapping of photosynthetic-related traits in rice under salt and alkali stresses

  • Jian Sun
  • Dongwei Xie
  • Enyuan Zhang
  • Hongliang Zheng
  • Jingguo Wang
  • Hualong Liu
  • Luomiang Yang
  • Shuli Zhang
  • Liang Wang
  • Detang ZouEmail author
Article
  • 36 Downloads

Abstract

Salt-alkali stress causes serious abiotic damage during the growth stage of rice. Photosynthetic characteristics and their related physiological traits affect the growth and development of rice under stressed conditions. In this study, using a recombinant inbred line (RIL) population derived from a cross between Dongnong 425 and Changbai 10 (CB10), quantitative trait loci (QTLs) for seven photosynthetic-related traits, including net photosynthetic rate (Pn), transpiration rate, stomatal conductance, intercellular CO2 concentration, chlorophyll content (Cc), canopy temperature and leaf area (La), were identified in control, salt and alkali stress conditions at the grain filling stage of rice over two years. In total, 23 QTLs were detected by using the CIM module of Windows QTL Cartographer 2.5 software, and 19 out of the 23 QTLs were salt-alkali stress-related. qPn3-2, qTr8, qCc3, qCc12 and qLa12 were specifically expressed in salt or alkali stress conditions. qTr6 expression was detected under both salt and alkali stresses for two consecutive years, suggesting it may have a key role in salt-alkali tolerance breeding. OsGLYII-2, which is a salt tolerant gene located in the mapping interval of major QTLs qPn3-2 and qCc3, was sequenced to verify the additive effect orientation of the two QTLs. The sequenced result showed that the OsGLYII-2 allele of extremely salt-tolerant RIL lines was consistent with that of the tolerant parent CB10 and showed that the positive alleles of qPn3-2 and qCc3 originated from CB10. The information obtained in this study may be useful for understanding the genetic basis of salt-alkali tolerance; it also provides an important base for the fine mapping and map-based cloning of the QTLs for photosynthetic-related traits under salt-alkali stress.

Keywords

Oryza sativa L. Salt Alkali Photosynthesis QTLs 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation (31701507), Youth Science Foundation of Heilongjiang Province (QC2017015), National Science and Technology Major Project (2018ZX0800912B-002), Postdoctoral Scientific Research Developmental Fund of Heilongjiang Province (LBH-Q17016), National Key Research and Development Plan (2017YFD0300501).

Author contributions

DZ and SJ designed research; SJ, DX, EZ, HZ, JW, HL, LY, SZ, LW performed research including phenotyping and genotyping; SJ wrote manuscript; DZ corrected manuscript. All authors have read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10681_2019_2470_MOESM1_ESM.xlsx (10 kb)
Supplementary file 1 (XLSX 10 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold RegionChinese Ministry of Education (Northeast Agricultural University)HarbinChina
  2. 2.Biotechnology Institute of Heilongjiang Academy of Agricultural SciencesHarbinChina
  3. 3.Kunming Tobacco CompanyKunmingChina

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