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Euphytica

, 215:145 | Cite as

Sodium azide mutagenesis generated diverse and broad spectrum blast resistance mutants in rice

  • Chang-Sheng WangEmail author
  • Kuan-Lin Lo
  • Arthur Z. Wang
Article
  • 23 Downloads

Abstract

Genetic mutagenesis can create novel alleles that do not exist in nature germplasms and has been widely used for the improvement of crop plants. Rice blast caused by Magnaporthe oryzae is one of the most destructive disease for rice production. For now, limited mutation-derived varieties associated with blast resistance were reported. In this study, a rice TNG67 mutant pool, developed by using sodium azide (SA) mutagenesis, contained more than 3000 pure mutant lines (SA mutants), were utilized. 50 SA mutants derived from rice blast susceptible variety TNG67 as well as other commercial and differential varieties were challenged with 89 blast isolates collected in Taiwan to understand their blast resistance spectrum. The results showed that SA mutants displayed different responses to M. oryzae, ranging from highly susceptible (6.7%) to highly resistant (100%). Seven mutants showed broad-spectrum resistance against the tested blast isolates. Bidirectional interaction analysis revealed a better understanding of plant–pathogen interaction. Our studies demonstrate that sodium azide mutagenesis is an efficient and effective method to induce rice blast resistance and generate valuable genetic resource for blast resistance improvement.

Keywords

Rice Blast disease Resistance mutant Mutagenesis Sodium azide 

Notes

Acknowledgements

We express our appreciation to the late Mr. Tung-Hai Tseng, whose contribution to this work was of great significance. We thank Mr. Yi-Chang Chang and Mrs. Lih-Jiuan Shieh for their contributions and providing pathogens in this study. Financial support partly from the Ministry of Science and Technology, Innovative Translational Agricultural Research Program from Academia Sinica, and the Higher Education Sprout Project by the Ministry of Education in Taiwan to C. S. Wang is highly appreciated.

Funding

Funding was provided by Ministry of Science and Technology, Taiwan (Grant No. MOST 107-2313-B-005-017), Academia Sinica (Grant Nos. 2014AG07s1, 2015AG07s1, 2016AG07s1, and 2017AG02s1), Ministry of Education (Grant No. 107S0204A).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10681_2019_2468_MOESM1_ESM.docx (40 kb)
Supplementary material 1 (DOCX 40 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Chang-Sheng Wang
    • 1
    • 2
    Email author
  • Kuan-Lin Lo
    • 1
  • Arthur Z. Wang
    • 1
  1. 1.Department of AgronomyNational Chung Hsing UniversityTaichungTaiwan
  2. 2.Advanced Plant Biotechnology CenterNational Chung Hsing UniversityTaichungTaiwan

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