Quantitative trait locus mapping for panicle exsertion length in common wheat using two related recombinant inbred line populations
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The improvement of panicle exsertion length (PEL) in wheat (Triticum aestivum L.) breeding programs is an emerging objective to improve yield potential, and serves as a complement to the wheat ideotype. In this study we aimed to advance the current understanding of genetic mechanisms underlying panicle exsertion in wheat. Two related recombinant inbred line populations sharing common parent H461 were used to identify and compare quantitative trait loci (QTL) controlling PEL. Using two high-density genetic linkage maps, 13 putative QTL for PEL were detected on chromosomes 1B, 1D, 2B, 2D, 4A, 4B, 5B, 6D, 7A, and 7B; each QTL explained 5.7–15.6% of the phenotypic variation. Among these 13 QTL, nine were independent; the other two pairs derived from H461 were validated to be common QTL based on newly developed high-resolution melt markers. Further, plant height (PH) was measured to perform covariance QTL analysis with eight major QTL; three of them derived from the wheat variety CM107 were not affected by PH. Pleiotropic effects on yield-related traits (PH, spike length, spikelet number per spike, spikelet density, kernel number per spike, kernel length, and thousand kernel weight) of the eight major QTL were also evaluated. These eight QTL may be valuable for fine mapping and marker-assisted selection in wheat breeding programs.
KeywordsPanicle exsertion length Plant height QTL Triticum aestivum Yield-related trait
Panicle exsertion length
Spikelet number per spike
Kernel number per spike
Thousand kernel weight
Recombinant inbred line population derived from the cross between H461 and CN16
Recombinant inbred line population derived from the cross between H461 and CM107
YT contributed to data analysis and drafted the manuscript. XY, YL and ZQW contributed to QTL analysis. FKW, XJJ, SHL, and MD performed the phenotypic evaluation and helped with data analysis. JM and GDC helped draft the manuscript. YMW participated in study design. YLZ coordinated the study and helped draft the manuscript. YXL designed and coordinated this study and revised the manuscript. All authors have read and approved the final manuscript.
This study was funded by the National Natural Science Foundation of China (31771794), the outstanding Youth Foundation of the Department of Science and Technology of Sichuan Province (2016JQ0040), the Key Technology Research and Development Program of the Department of Science and Technology of Sichuan Province (2016NZ0057), and the International Science & Technology Cooperation Program of the Bureau of Science and Technology of Chengdu China (No. 2015DFA306002015-GH03-00008-HZ).
Compliance with ethical standards
The experiments comply with the ethical standards in the country in which they were performed.
Conflict of interest
The authors declare that they have no conflict of interest.
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