Cotton (Gossypium hirsutum L.) mutants with reduced levels of palmitic acid (C16:0) in seed lipids
- 68 Downloads
The objective of this study was to identify mutants of upland cottonseed (Gossypium hirsutum L.) with reduced levels of palmitic acid (C16:0) in the seed. The five parental populations that had been exposed to Ethyl MethaneSulfonate in 1997 or 2002 were initially screened for either herbicide tolerance or divergent fiber quality. Fatty acid composition were determined for five seed bulks from 140 M5 mutant lines in 2009 and 100 M6 partial seeds in 2010. Mutants with reduced levels of palmitic acid levels were isolated from TAM 94L-25 (PI 631440), Acala 1517-99 (Reg. No. CV-115, PI 612326) and TTU SCM3-7-3 (Reg. No.GS-4, PI 657942). Thirteen M6 single seeds with reduced levels of palmitic acid (17.9–19.6%) were intercrossed in the greenhouse. Ten of the single seed selections were designated as potential male parents and randomly crossed to three potential female parents to generate F1 plants and 21 F2 populations for allele testing. Based on these tests, five F2 populations with reduced levels of low palmitic acid, were selected for further evaluation. The development of mutant lines with reduced levels of palmitic acid in upland cotton was submitted for proprietary protection under U.S. Patent No. 20160222399.
KeywordsReduced levels of palmitic acid Fatty acid composition of cottonseed oil Chemical mutagenesis
Research supported for this work was provided by funding of Cotton Incorporated, the TTU Cotton Research Line of the USDA, the TTU Plant and Soil Science Department Rockwell Endowed Chair, and the TTU Office of Research Commercialization. Offsite chemical analyses was conducted at the USDA-ARS Southern Regional Research Center in New Orleans, LA and the Biodiversity Institute at the University of North Texas in Denton, TX. This research required 15 years and included the research efforts of 11 graduate students and six collaborating scientists scattered across the Southern U.S.A. Special thanks goes to Dr. Kater Hake, Vice President of Agricultural and Environmental Research at Cotton Incorporated, for providing coordination, moral support, and funding for this research team. “Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer”.
Compliance with ethical standards
Conflict of interest
The reduced palmitic acid mutant identified in this study are protected by U.S. Patent Application No. 20160222399 (March 8, 2014) (Auld and Hendon 2016). Distribution of these lines will require permission of the Office of Research Commercialization at Texas Tech University, Box 42007, Lubbock, TX 79409 (806-742-4105).
- AOCS (1998) Official methods and recommended practices of the AOCS. In: Firestone D (ed) 5th edn. American Oil Chemists’ Society, Champaign, p 3Google Scholar
- Auld DL, Hendon BR (2016) U.S. Patent No. 20160222399. Low Palmitic Acid Cotton Lines. Texas Tech University, Office of Technology Commercialization. Filed March 8, 2014Google Scholar
- Bowman R (2013) Soil temperatures effect planting decisions. Southwest Farm Press. http://www.southwestfarmpress.com/cotton/. Accessed 13 Nov 2018
- Cantrell RG, Roberts CL, Waddell C (2000) Registration of ‘Acala 1517-99’. Cotton Crop Sci 40:1199–1200Google Scholar
- Christie WW (1982) Lipid analysis, 2nd edn. Pegamon Press, New York, pp 52–54Google Scholar
- Dowd MK, Boykin DL, Meredith WR Jr, Campbell BT, Bourland FM, Gannaway JR, Glass KM, Zhang J (2010) Fatty acid profiles of cottonseed genotypes from the national cotton variety trials. J Cotton Sci 14:64–73Google Scholar
- Green A, Singh S, Liu Q (2009) Modified cottonseed oil. United States Patent 7619105. November, 17Google Scholar
- Liu Q, Singh S, Chapman K, Green A (2009) Bridging traditional and molecular genetics in modifying cottonseed oil. In: Paterson AH (ed) Plant genetics and genomics: crops and models, vol 3. Genetics and Genomics of Cotton. Springer, pp 353–382Google Scholar
- O’Brien RD (2002) Cottonseed Oil. In: Gunstone FD (ed) Vegetable oil in food technology: composition, properties and uses. Blackwell Publishing, Oxford, pp 203–230Google Scholar
- USDA-ERS (2016) Oil crops yearbook. Table 47. http://www.ers.usda.gov/data-products/oil-crops-yearbook.aspx. Accessed 19 May 2016