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Euphytica

, 215:45 | Cite as

Diversification of seed carotenoid content and profile in wild barley (Hordeum chilense Roem. et Schultz.) and Hordeum vulgare L.–H. chilense synteny as revealed by DArTSeq markers

  • C. M. Avila
  • M. G. Mattera
  • C. Rodríguez-Suárez
  • C. Palomino
  • M. C. Ramírez
  • A. Martin
  • A. Kilian
  • D. Hornero-Méndez
  • S. G. AtienzaEmail author
Article

Abstract

The high carotenoid content and distinctive carotenoid profile of tritordeum are conferred by its wild progenitor, Hordeum chilense. Genetic studies on this wild barley could exploit the knowledge gained in Hordeum vulgare L. if the synteny between H. vulgare and H. chilense is established. DArTSeq markers were aligned to barley genome and used to inspect H. chilense-barley synteny. All chromosome pairs showed a good degree of collinearity with the exception of 7Hv–7Hch, where a reciprocal translocation in 7Hch was identified. Carotenoid analyses revealed a high diversity for total carotenoids, free and esterified lutein in a collection of H. chilense. Population structure analyses revealed the existence of two subgroups contrasting for total carotenoids, free lutein and esterified lutein in seeds. Lutein esters were produced with palmitic and linoleic acids as happens in tritordeum. However, tritordeum prefer palmitic acid for the synthesis of lutein esters but this preference is not maintained in H. chilense. This indicates the existence of diversity in the enzymes involved in the esterification which could be useful in tritordeum breeding. Furthermore, several accessions produced lutein monoesters but they lacked diesters which suggests that esterification is controlled by more than one enzyme in H. chilense. A total of 91 marker-trait associations were identified for carotenoid content and profile. These associations constitute a good starting point for future genetic analyses for the identification of candidate genes from H. vulgare genome.

Keywords

Carotenoid esters Lutein esters Hordeum chilense Tritordeum Wild relatives 

Notes

Acknowledgements

Research funded by Grant AGL2014-53195R, from Ministerio de Economía y Competitividad, Spain (MINECO) including FEDER funding. M.G.M. was recipient of FPI (BES-2012-055961). D.H.-M. is a member of CaRed Network, funded by MINECO (BIO2015-71703-REDT). S.G.A. and CMA are members of FiRCMe Network, funded by MINECO (AGL2016-81855-REDT).

Supplementary material

10681_2019_2369_MOESM1_ESM.xlsx (362 kb)
Supplementary file 1. Alignment results of DArTSeq markers against barley genes. (XLSX 361 kb)
10681_2019_2369_MOESM2_ESM.xlsx (241 kb)
Supplementary file 2. DArTSeq markers showing significant association after GWAS analysis. (XLSX 241 kb)
10681_2019_2369_MOESM3_ESM.xlsx (11 kb)
Supplementary file 3. Comparison of the resulting groups in H. chilense with DArTseq markers and morphological characters. (XLSX 10 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • C. M. Avila
    • 1
  • M. G. Mattera
    • 2
    • 4
  • C. Rodríguez-Suárez
    • 2
  • C. Palomino
    • 2
  • M. C. Ramírez
    • 2
  • A. Martin
    • 2
  • A. Kilian
    • 3
  • D. Hornero-Méndez
    • 4
  • S. G. Atienza
    • 2
    Email author
  1. 1.Área Mejora y BiotecnologíaIFAPA-Centro Alameda del ObispoCórdobaSpain
  2. 2.Institute for Sustainable AgricultureCSICCórdobaSpain
  3. 3.Diversity Arrays TechnologyUniversity of CanberraBruceAustralia
  4. 4.Departament of Food PhytochemistryInstituto de la Grasa (CSIC)SevilleSpain

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