, 215:43 | Cite as

Molecular variation at BvBTC1 is associated with bolting tolerance in Japanese sugar beet

  • Yosuke KurodaEmail author
  • Hiroyuki Takahashi
  • Kazuyuki Okazaki
  • Kazunori Taguchi


Bolting tolerance is an important breeding trait in sugar beets (Beta vulgaris). To understand factors controlling bolting tolerance in Japanese sugar beets, we focused on the molecular variation of the BvBTC1 gene, a master gene linked to life cycle (annual vs. biennial). Although the biennial haplotype “a” is recognized in beets worldwide, our genomic sequencing detected two additional haplotypes, an annual haplotype “g” and a novel haplotype we named “o”, which is similar to the haplotype found in annual beet. We developed cleaved amplified polymorphic sequence markers to discriminate haplotypes ‘a’, ‘g’ and ‘o’. Their correlation with bolting tendency was investigated by analyzing bolting-induced beets. In segregated genotypes (hybrid or strains), we found that bolting plants tended to possess a lower proportion of the biennial “aa” genotype than did non-bolting plants. We also observed that bolting tolerance varied among inbred breeding strains (strains whose genotypes were mostly fixed). Genotype frequencies of “aa” were lower in strains with weaker bolting tolerances (88.9% frequency for strong tolerance, 42.2% for medium tolerance, and 19.3% for weak tolerance). Therefore, molecular variation in the BvBTC1 gene can be a target for improving bolting tolerance in Japanese sugar beets.


Bolting tolerance BvBTC1 Genotype Japanese sugar beet 



The authors thank the staff of the experimental farms at HARC for their assistance with field experiments. The authors also appreciate the support from the Grant-in-Aid for Young Scientists (B) Grant Number JP25871104 and Grant-in-Aid for Scientific Research (C) Grant Number JP17K07616 of the Japan Society for the Promotion of Science.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Yosuke Kuroda
    • 1
    Email author
  • Hiroyuki Takahashi
    • 1
  • Kazuyuki Okazaki
    • 1
  • Kazunori Taguchi
    • 1
  1. 1.Sugar beet breeding group Hokkaido Agricultural Research CenterNational Agriculture and Food Research OrganizationKasaiJapan

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