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Euphytica

, 215:39 | Cite as

Genetic analysis of environment-sensitive genic male sterile rice under US environments

  • Christian T. De GuzmanEmail author
  • Steven D. Linscombe
  • James H. Oard
Article
  • 17 Downloads

Abstract

Two line hybrid rice (Oryza sativa L.) breeding uses environment-sensitive genic male sterile (EGMS) lines to produce sterile or fertile pollen depending on daylength and/or temperature. There is limited information on the performance and genetic control of EGMS lines under U.S. environments. Therefore, genetic characterization of two F2 and four BC1F2 populations derived from EGMS line 2009S was conducted under Louisiana field conditions. Chi squared analyses in the F2 and BC1F2 populations indicated that pollen sterility under high temperature and long daylength field conditions was controlled by a single recessive gene. Sequence comparisons at locus LOC_Os02g12290 between 2009S, CL161 (USDA-AMS 2002) and published sequences of G63S and Nipponbare revealed a single nucleotide polymorphism (SNP) that has been detected previously in several EGMS lines. Due to high GC content, a CEL1 nuclease assay was used to detect SNPs associated with pollen sterility in 177 F2 and 59 BC1F2 sampled individuals. A high percentage of lines (90–100%) across all segregating populations were identified correctly as pollen sterile using the CEL 1 assay. Results from this study suggest that single-gene control of pollen sterility in EGMS line 2009S will be compatible with a two-line system for U.S. hybrid rice development.

Keywords

Rice Hybrid Male sterility Single nucleotide polymorphism (SNP) 

Notes

Supplementary material

10681_2019_2363_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 15 kb)

References

  1. Alcochete AAN, Rangel PHN, Ferreira ME (2005) Mapping of quantitative trait loci for thermosensitive genic male sterility in indica rice. Pesq Agropecu Bras 40:1179–1188.  https://doi.org/10.1590/s0100-204x2005001200004 CrossRefGoogle Scholar
  2. Arkansas Rice Breeders Join 5-State Consortium To Develop Hybrids. http://www.mafg.net/Files/Arkansas%20Rice%20Breeders%20Join%205-State%20Consortium%20To%20Develop%20HybridsyqXXUc.pdf. Accessed 13 Jan 2018
  3. Barkley NA, Wang ML (2008) Application of TILLING and EcoTILLING as reverse genetic approaches to elucidate the function of genes in plants and animals. Curr Genomics 9:212–226.  https://doi.org/10.2174/138920208784533656 CrossRefPubMedPubMedCentralGoogle Scholar
  4. Chen L, Liu YG (2014) Male sterility and fertility restoration in crops. Annu Rev Plant Biol 65:579–606.  https://doi.org/10.1146/annurev-arplant-050213-040119 CrossRefPubMedGoogle Scholar
  5. Chen LB, Zhou GQ, Huang YX (1994) Influence of temperature and photoperiod on the fertility and physiology of Annong S-1 and Hengnong S-1 of rice. Acta Bot Sin 36(Suppl.):119–123Google Scholar
  6. Chonlawat P, Prapa S, Fisseha W, Tanee S (2016) Genetic background screening to accelerate backcross breeding program for TGMS lines development. J Plant Sci 11:86–90CrossRefGoogle Scholar
  7. De Guzman CT (2016) Genetic analyses of male sterility and wide compatibility in U.S. hybrid rice breeding lines. LSU Doctoral Dissertations. 3418. https://digitalcommons.lsu.edu/gradschool_dissertations/3418
  8. De Guzman C, Esguerra M, Linscombe S, Berger G, Sha X, Oard J (2017) Genetic analysis of photoperiod/thermosensitive male sterility in rice under US environments. Crop Sci 57:1957–1965.  https://doi.org/10.2135/cropsci2016.11.0950 CrossRefGoogle Scholar
  9. Ding J, Lu Q, Ouyang Y, Mao H, Zhang P, Yao J et al (2012) A long noncoding RNA regulates photoperiod-sensitive male sterility, an essential component of hybrid rice. Proc Natl Acad Sci USA 109:2654–2659.  https://doi.org/10.1073/pnas.1121374109 CrossRefPubMedGoogle Scholar
  10. Gawel NJ, Jarret RL (1991) A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Mol Biol Report 9:262–266.  https://doi.org/10.1007/bf02672076 CrossRefGoogle Scholar
  11. Gramene (2016) Gramene: a comparative resource for plants. Gramene. http://www.gramene.org. Accessed 7 Dec 2017
  12. Haider Z, Akhter M, Riaz M, Sabar M, Latif T (2014) Hybrid rice production in Pakistan: assessments of limitations and potentials. In: Clewley J (ed) Hybrid rice production in Asia: assessments of limitations and potentials. Food and Agriculture Organization of the United Nations Regional Office for Asia and the Pacific, p 21Google Scholar
  13. He YQ, Yang J, Xu CG, Zhang ZG, Zhang Q (1999) Genetic bases of instability of male sterility and fertility reversibility in photoperiod-sensitive genic male-sterile rice. Theor Appl Genet 99:683–693CrossRefGoogle Scholar
  14. Jensen MA, Fukushima M, Davis RW (2010) DMSO and betaine greatly improve amplification of GC-rich constructs in de novo synthesis. PLoS ONE 5(6):e11024.  https://doi.org/10.1371/journal.pone.0011024 CrossRefPubMedPubMedCentralGoogle Scholar
  15. Jiang G-Q, Yao X-F, Liu C-M (2013) A simple CELI endonuclease-based protocol for genotyping both SNPs and InDels. Plant Mol Biol Rep 31:1325–1335.  https://doi.org/10.1007/s11105-013-0606-z CrossRefGoogle Scholar
  16. Kadaru SB, Yadav AS, Fjellstrom RG, Oard JH (2006) Alternative ecotilling protocol for rapid, cost-effective single-nucleotide polymorphism discovery and genotyping in rice (Oryza sativa L.). Plant Mol Biol Rep 24:3–22.  https://doi.org/10.1007/bf02914042 CrossRefGoogle Scholar
  17. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948CrossRefGoogle Scholar
  18. Lopez MT, Toojinda T, Vanavichit A, Tragoonrung S (2003) Microsatellite markers flanking the tms2 gene facilitated tropical TGMS rice line development. Crop Sci 43:2267–2271.  https://doi.org/10.2135/cropsci2003.2267 CrossRefGoogle Scholar
  19. Mei G, Wang X, Wang M (1990) Genetic analysis of the photoperiod sensitive male sterility of Nongken 58S and its derivatives. J Huangzhong Agric Univ 9:400–406Google Scholar
  20. Mei MH, Chen L, Zhang ZH, Li ZY, Xu CG, Zhang Q (1999a) pms3 is the locus causing the original photoperiodsensitive male sterility mutation of “Nongken 58S”. Sci China (Ser C) 42:316–322.  https://doi.org/10.1007/bf03183609 CrossRefGoogle Scholar
  21. Mei MH, Dai XK, Xu CG, Zhang Q (1999b) Mapping and genetic analysis of the genes for photoperiod-sensitive genic male sterility in rice using the original mutant nongken 58S. Crop Sci 39:1711–1715.  https://doi.org/10.2135/cropsci1999.3961711x CrossRefGoogle Scholar
  22. Muthayya S, Sugimoto JD, Montgomery S, Maberly GF (2014) An overview of global rice production, supply, trade, and consumption. Ann NY Acad Sci 1324:7–14CrossRefGoogle Scholar
  23. Nalley L, Tack J, Barkley A, Jagadish K, Brye K (2016) Quantifying the agronomic and economic performance of hybrid and conventional rice varieties. Agron J 108:1514–1523.  https://doi.org/10.2134/agronj2015.0526 CrossRefGoogle Scholar
  24. Oleykowski CA, Bronson Mullins CR, Godwin AK, Yeung AT (1998) Mutation detection using a novel plant endonuclease. Nucleic Acids Res 26:4597–4602CrossRefGoogle Scholar
  25. Peng HF, Chen XH, Lu YP, Peng YF, Wan BH, Chen ND (2010) Fine mapping of a gene for non-pollen type thermosensitive genic male sterility in rice (Oryza sativa L.). Theor Appl Genet 120:1013–1020.  https://doi.org/10.1007/s00122-009-1229-5 CrossRefPubMedPubMedCentralGoogle Scholar
  26. Qi Y, Liu Q, Zhang L, Mao B, Yan D, Jin Q (2014) Fine mapping and candidate gene analysis of the novel thermo-sensitive genic male sterility tms9-1 gene in rice. Theor Appl Genet 127:1173–1182.  https://doi.org/10.1007/s00122-014-2289-8 CrossRefPubMedPubMedCentralGoogle Scholar
  27. Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, Rozen SG (2012) Primer3—new capabilities and interfaces. Nucleic Acids Res 40(15):e115.  https://doi.org/10.1093/nar/gks596 CrossRefPubMedPubMedCentralGoogle Scholar
  28. USDA-AMS (2002) Application for plant variety protection. 26 June 2002. USDA, Agric. Marketing Services. https://apps.ams.usda.gov/CMS/AdobeImages/200200198.pdf. Accessed 5 Oct 2016
  29. Xie FM, He ZH, Esguerra MQ, Qiu FL, Ramanathan V (2013) Determination of heterotic groups for tropical indica hybrid rice germplasm. Theor Appl Genet 127(2):407–417CrossRefGoogle Scholar
  30. Xu J, Wang B, Wu Y, Du P, Wang J, Wang M (2011) Fine mapping and candidate gene analysis of ptgms2-1, the photoperiod-thermo-sensitive genic male sterile gene in rice (Oryza sativa L.). Theor Appl Genet 122:365–372.  https://doi.org/10.1007/s00122-010-1452-0 CrossRefPubMedGoogle Scholar
  31. Yan ZB, Yan WG, Deren CW, McClung A (2010) Hybrid rice breeding. B.R. Wells rice research studies. AAES Res Ser 591:61–63Google Scholar
  32. Yang RC, Liang K, Wang N, Chen S (1992) A recessive gene in indica rice 5460S for thermosensitive genic male sterility. Rice Genet Newsl 9:56–57Google Scholar
  33. Yuan LP (1990) Progress of two-line system hybrid rice breeding. Sci Agric Sin 23:6Google Scholar
  34. Zhang Q, Shen BZ, Dai XK, Mei MH, Saghai Maroof MA, Li ZB (1994) Using bulked extremes and recessive class to map genes for photoperiod-sensitive genic male sterility in rice. Proc Natl Acad Sci USA 91:8675–8679.  https://doi.org/10.1073/pnas.91.18.8675 CrossRefPubMedGoogle Scholar
  35. Zhang H-L, Huang J-Z, Liu Q-L, Nawaz Z, Lu H-P, Gong J-Y, Zhu Y-J, Yan W, Shu Q-Y (2014) Characterization of an RNase Z nonsense mutation identified exclusively in environment-conditioned genic male sterile rice. Mol Breed 34:481–489CrossRefGoogle Scholar
  36. Zhou YF, Zhang XY, Xue QZ (2011) Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice. J Zhejiang Univ Sci B 12:436–447.  https://doi.org/10.1631/jzus.B1000306 CrossRefPubMedPubMedCentralGoogle Scholar
  37. Zhou H, Liu Q, Li J, Jiang D, Zhou L, Wu P (2012) Photoperiod and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA. Cell Res 22:649–660.  https://doi.org/10.1038/cr.2012.28 CrossRefPubMedPubMedCentralGoogle Scholar
  38. Zhou H, Zhou M, Yang Y, Li J, Zhu L, Jiang D (2014) RNase Z(S1) processes UbL40 mRNAs and controls thermosensitive genic male sterility in rice. Nat Commun 5:4884.  https://doi.org/10.1038/ncomms5884 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Christian T. De Guzman
    • 1
    Email author
  • Steven D. Linscombe
    • 2
  • James H. Oard
    • 2
  1. 1.Southeast Missouri State UniversityCape GirardeauUSA
  2. 2.Louisiana State University Agricultural CenterRayneUSA

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