Validation of the effects of the Gpc-B1 high grain protein concentration locus from Lillian hard red spring wheat (Triticum aestivum L.) using locus specific markers
Wheat grain protein concentration (GPC) is negatively associated with economic grain yield (GY) hindering attempts to improve these traits simultaneously. Studies indicate the incorporation of the Gpc-B1 high grain protein gene from emmer wheat into commercial wheat cultivars improves grain quality and nitrogen use efficiency with little effect on GY. Molecular markers are helpful in tracking such genes in breeding lines. Our objectives were to study the cultivar Lillian which has Xucw71 and Xuhw89 marker molecular variants commonly associated with Gpc-B1, in order to (1) validate the efficacy of the genetic markers for selecting Gpc-B1 associated with high GPC from Lillian, and (2) assess the effect of the presence of the DNA segment linked to these markers on GPC, GY and yield related traits in genetic populations. Four genetic populations deriving from the Gpc-B1 donor Lillian were developed and partitioned using markers Xucw71 and Xuhw89 into two groups of lines, one group bearing homozygous Gpc-B1 (M+) and the second the homozygous null (M−) allele. Field tests were conducted at Swift Current and Stewart Valley, Saskatchewan, and Lethbridge, Alberta over 2 years. Gpc-B1 significantly influenced GPC, GY, thousand kernel weight (TKW) and test weight, but its effect on plant maturity was inconsistent showing variable effects in different populations and environments. Significantly higher GPC (P ≤ 0.0001) was detected in lines with the M+ than the M− allele. Genetic markers Xucw71 and Xuhw89 were effective in discriminating Gpc-B1 from Lillian confirming their value in marker assisted breeding.
KeywordsXucw71 Xuhw89 DNA markers Protein content Triticum
We gratefully acknowledge the financial support of the producer funded Wheat Check-off (administered by the Western Grains Research Foundation) and Agriculture and Agri-Food Canada and technical assistance provided by members of the wheat breeding and molecular biology research group at SCRDC-AAFC is sincerely appreciated.
- Avivi L (1978) High protein concentration in wild tetraploid Triticum dicoccoides Korn. In: Ramanujam S (ed) Proceedings 5th international wheat genetics symposium Indian society of genetics and plant breeding, New Delhi, pp 372–380Google Scholar
- Blanco A, Mangini G, Giancaspro A, Giove S, Colasuonno P, Simeone R, Signorile A, De Vita P, Mastrangelo AM, Cattivelli L, Gadaleta A (2012) Relationships between grain protein content and grain yield components through quantitative trait locus analyses in a recombinant inbred line population derived from two elite durum wheat cultivars. Mol Breed 30:79–92CrossRefGoogle Scholar
- Brown PD, Randhawa HS, Fetch JM, Meiklejohn M, Fox SL, Humphreys DG, Green D, Wise I, Fetch T, Gilbert J, McCallum B, Menzies J (2017) Conquer red spring wheat. Can J Plant Sci 97:147–152Google Scholar
- Cuthbert RD (2014) AAC Connery. http://www.inspection.gc.ca/english/plaveg/pbrpov/cropreport/whe/app00009619e.shtml. Accessed 26 May 2018
- Hucl P (2006) CDC Alsask. http://www.inspection.gc.ca/english/plaveg/pbrpov/cropreport/whe/app00006287e.shtml. Accessed 26 May 2018
- Marker assisted selection (MAS) in wheat. http://maswheat.ucdavis.edu/protocols/HGPC/. Accessed 30 Apr 2018
- Pallotta MA, Warner P, Fox RL, Kuchel H, Jeffries SP, Langridge P (2003) Marker assisted wheat breeding in the southern region of Australia. In: Pogna NE, Romano M, Pogna EA, Galterio G (eds) Proceedings 10th international wheat genetics symposium. Paestum, Italy. Istituto Sperimentale per La Cerealicoltura Roma pp 789–791Google Scholar
- SAS Institute Inc (2004) SAS/STAT® 9.1 User’s Guide. Cary, NC: SAS Institute Inc.Google Scholar
- Shiaoman Chao and Daryl Somers. https://www.yumpu.com/en/document/view/11880064/wheat-and-barley-dna-extraction-in-96-well-plates-maswheat. Accessed 22 Apr
- Uauy C, Brevis JC, Chen X, Khan I, Jackson L, Chicaiza O, Distelfeld A, Fahima T, Dubcovsky J (2005) High temperature adult-plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1. Theor Appl Genet 122:97–105CrossRefGoogle Scholar
- Williams PC (1979) Screening wheat for protein and hardness by near infrared reflectance spectroscopy. Cereal Chem 56:169–172Google Scholar