, 214:233 | Cite as

Geno-and phenotyping of submergence tolerance and elongated uppermost internode traits in doubled haploids of rice

  • Lovelin Kaushal
  • K. Ulaganathan
  • Vinay Shenoy
  • S. M. BalachandranEmail author


The study was undertaken to explore the potential of doubled haploidy in fixation of dominant (submergence tolerance) and recessive (elongated uppermost internode) traits. Doubled haploids were produced by anther culture of F1 hybrids involving genotypes possessing sub1 and eui genes. The genotyping and phenotyping of traits were confirmed both at molecular and phenotypic levels. The molecular markers ART5 and SC3 were sufficient enough to screen for presence of Sub-1 gene, whereas RM 5970 could screen eui gene. Screening of DH lines for the targeted traits Sub-1 and eui, revealed the improvement of traits among DH lines. DH lines with submergence tolerant gene (Sub1) had significantly higher survival rate compared to susceptible checks, but on par with the Sub-1 parents. The tested genotypes exhibited considerable variation for eui trait. The significant variation for eui trait expression was observed among the DH lines. The degree of panicle exertion was on par with the donor parents and varied among the DH lines The study clearly indicates the scope for fixation of both dominant and recessive genes through doubled haploidy.


Anther culture Doubled haploidy Genetic fixation Submergence Elongated uppermost internodes 



Authors are thankful to Dr. Usha B. Zehr, Director, Barwale Foundation and the Director, IIRR, Hyderabad for providing facilities for the execution of this research work.

Authors contribution

Lovelin Kaushal Actual execution of research work including anther culture, production of doubled haploids, characterization of double haploids and their parents with molecular markers and evaluation of doubled haploids for the targeted traits. K. Ulaganathan Helped in intellectual inputs like designing primers and molecular analysis of doubled haploids. V. Shenoy Helped in molecular analysis of doubled haploids, genetic interpretation of data and experiments related to breeding rice. S.M. Balachandran Overall conception of the research topic, designing of experiments, guiding the first author in execution of the work including production, characterization and evaluation of doubled haploids and their utility in rice breeding; drafting, editing, finalization and submission of the manuscript for publication.

Compliance with ethical standards

Conflict of interest

Authors certify that they do not have any conflict of interest with reference to the submitted manuscript.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Lovelin Kaushal
    • 1
  • K. Ulaganathan
    • 2
  • Vinay Shenoy
    • 3
  • S. M. Balachandran
    • 4
    Email author
  1. 1.Syngenta India LimitedHyderabadIndia
  2. 2.Centre for Plant Molecular BiologyOsmania UniversityHyderabadIndia
  3. 3.Kaveri Seed Company LimitedSecunderabadIndia
  4. 4.Crop Improvement SectionICAR-Indian Institute of Rice ResearchRajendranagar, HyderabadIndia

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