Molecular cytogenetic characterization of an Agropyron cristatum 6PL chromosome segment conferring superior kernel traits in wheat
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The wild relative of wheat, Agropyron cristatum (L.) Gaertn. (genome PPPP), acts as an important genetic resource for providing valuable genes for wheat improvement and increasing the diversity of cultivated wheat. In the present study, cytogenetic and molecular tools were used to characterize wheat-A. cristatum 6P derivatives. The novel translocation line Pubing260 exhibiting wider flag leaves and superior spike traits was further characterized and analysed. Cytological studies demonstrated that Pubing260 (2n = 42) contained a T3BL·3BS-6PL terminal translocation. We compared molecular markers on chromosomes 6P and 3B and confirmed that the translocated wheat chromosome was 3BS and that the chromosome breakage occurred in bin 3BS9-0.57-0.75. Compared with its recurrent parent Fukuhokomugi, Pubing260 had a wider flag leaf, more spikelets and more grains per spike in two growing seasons. Genetic analysis conducted using BC1F2 and BC2F1 populations suggested that the A. cristatum chromosomal fragment from bin 6PL-0.72-1.00 conferred these potentially valuable agronomic traits. On average, the flag leaf width (FLW), and numbers of grain per spike (GNS), spikelets per spike (SNS) and kernels per spikelet of plants with the translocation were 2 mm wider and 5.1, 0.8 and 0.3 higher, respectively, than those of plants lacking the translocation in segregating populations. Significant and positive correlations were observed among GNS, SNS and FLW. In summary, this study not only reports a novel germplasm that is potentially valuable for wheat improvement but also enriches the genetic resources of wheat.
KeywordsKernel number Molecular cytogenetics Triticum aestivum Wide flag leaf
This work was supported by grants from China Agriculture Research System (CARS-03) and the National Key Research and Development Program of China (2016YFD0102000).
LLH conceived the research. ZJ, MHH performed the research. ZJ wrote the paper. LWH produced the translocation lines. ZJP, ZSH, HHM, LXQ and YXM participated in the preparation of the reagents and materials used in the study.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- Brenchley R, Spannagl M, Pfeifer M, Barker GLA, D’Amore R, Allen AM, McKenzie N, Kramer M, Kerhornou A, Bolser D, Kay S, Waite D, Trick M, Bancroft I, Gu Y, Huo N, Luo M-C, Sehgal S, Gill B, Kianian S, Anderson O, Kersey P, Dvorak J, McCombie WR, Hall A, Mayer KFX, Edwards KJ, Bevan MW, Hall N (2012) Analysis of the bread wheat genome using whole-genome shotgun sequencing. Nature 491:705–710CrossRefGoogle Scholar
- Ding X, Li X, Xiong L (2011) Evaluation of near-isogenic lines for drought resistance QTL and fine mapping of a locus affecting flag leaf width, spikelet number, and root volume in rice. Theor Appl Genet 123:815–826. https://doi.org/10.1007/s00122-011-1629-1 CrossRefPubMedPubMedCentralGoogle Scholar
- Gaju O, Reynolds MP, Sparkes DL, Mayes S, Ribas-Vargas G, Crossa J, Foulkes MJ (2014) Relationships between physiological traits, grain number and yield potential in a wheat DH population of large spike phenotype. Field Crops Res 164:126–135. https://doi.org/10.1016/j.fcr.2014.05.015 CrossRefGoogle Scholar
- Huang C, Zhang J-P, Liu W-H, Yang X-M, Li X-Q, Lu Y-Q, Li L-H, Gao A-N (2013) Identification of wheat-Agropyron cristatum 6P chromosome intercalary translocation lines. J Plant Genet Res 14:606–611Google Scholar
- Kang H-Y, Zhang Z-J, Xu L-L, Qi W-L, Tang Y, Wang H, Zhu W, Li D-Y, Zeng J, Wang Y, Fan X, Sha L-N, Zhang H-Q, Zhou Y-H (2016) Characterization of wheat-Psathyrostachys huashanica small segment translocation line with enhanced kernels per spike and stripe rust resistance. Genome 59:221–229. https://doi.org/10.1139/gen-2015-0138 CrossRefPubMedGoogle Scholar
- Kuzmanović L, Gennaro A, Benedettelli S, Dodd IC, Quarrie SA, Ceoloni C (2014) Structural-functional dissection and characterization of yield-contributing traits originating from a group 7 chromosome of the wheatgrass species Thinopyrum ponticum after transfer into durum wheat. J Exp Bot 65:509–525. https://doi.org/10.1093/jxb/ert393 CrossRefPubMedGoogle Scholar
- Kuzmanović L, Ruggeri R, Virili ME, Rossini F, Ceoloni C (2016) Effects of Thinopyrum ponticum chromosome segments transferred into durum wheat on yield components and related morpho-physiological traits in Mediterranean rain-fed conditions. Field Crops Res 186:86–98. https://doi.org/10.1016/j.fcr.2015.11.007 CrossRefGoogle Scholar
- Li L-H, Li X-Q, Li P, Dong Y-C, Zhao G-S (1997) Establishment of wheat-Agropyron cristatum alien addition lines. I. Cytology of F3, F2, BC1, BC4, and BC3F1 progenies. Acta Genet Sin 24:154–159 (in Chinese with an English abstract) Google Scholar
- Li L, Yang X, Li X, Dong Y, Chen X (1998) Introduction of desirable genes from Agropyron cristatum into common wheat by intergeneric hybridization. Sci Agric Sin 31:1–6Google Scholar
- Molnár-Láng M, Ceoloni C, Doležel J (2015) Alien introgression in wheat-cytogenetics, molecular biology, and genomics. Springer International Publishing, ChamGoogle Scholar
- Qi W, Tang Y, Zhu W, Li D, Diao C, Xu L, Zeng J, Wang Y, Fan X, Sha L, Zhang H, Zheng Y, Zhou Y, Kang H (2016) Molecular cytogenetic characterization of a new wheat-rye 1BL·1RS translocation line expressing superior stripe rust resistance and enhanced grain yield. Planta 244:405–416. https://doi.org/10.1007/s00425-016-2517-3 CrossRefPubMedGoogle Scholar
- Ru Z, Feng S, Li G (2015) High-yield potential and effective ways of wheat in Yellow & Huai River Valley Facultative Winter Wheat Region. Sci Agric Sin 48:3388–3393Google Scholar
- Sakamoto T, Morinaka Y, Ohnishi T, Sunohara H, Fujioka S, Ueguchi-Tanaka M, Mizutani M, Sakata K, Takatsuto S, Yoshida S, Tanaka H, Kitano H, Matsuoka M (2006) Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat Biotechnol 24:105–109CrossRefGoogle Scholar
- Sears ER, Gustafson JP (1993) Use of radiation to transfer alien chromosome segments to wheat. Crop Sci 33:897–901. https://doi.org/10.2135/cropsci1993.0011183X003300050004x CrossRefGoogle Scholar
- Song L, Lu Y, Zhang J, Pan C, Yang X, Li X, Liu W, Li L (2016a) Cytological and molecular analysis of wheat-Agropyron cristatum translocation lines with 6P chromosome fragments conferring superior agronomic traits in common wheat. Genome 59:840–850. https://doi.org/10.1139/gen-2016-0065 CrossRefPubMedGoogle Scholar
- Wu Q, Chen Y, Fu L, Zhou S, Chen J, Zhao X, Zhang D, Ouyang S, Wang Z, Li D, Wang G, Zhang D, Yuan C, Wang L, You M, Han J, Liu Z (2016) QTL mapping of flag leaf traits in common wheat using an integrated high-density SSR and SNP genetic linkage map. Euphytica 208:337–351. https://doi.org/10.1007/s10681-015-1603-0 CrossRefGoogle Scholar
- Xue S, Zhang Z, Lin F, Kong Z, Cao Y, Li C, Yi H, Mei M, Zhu H, Wu J, Xu H, Zhao D, Tian D, Zhang C, Ma Z (2008b) A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor Appl Genet 117:181–189. https://doi.org/10.1007/s00122-008-0764-9 CrossRefPubMedGoogle Scholar