Linkage map construction and QTL identification of P-deficiency tolerance in Oryza rufipogon Griff. at early seedling stage
- 210 Downloads
Low phosphorus availability is a major factor limiting rice productivity. In this study, a population of backcross recombinant inbred lines (BILs) derived from an inter-specific cross (Oryza sativa L. × O. rufipogon Griff.) was used for genetic linkage map construction and quantitative trait locus (QTL) mapping. The results showed that a linkage map consisting of 153 markers was constructed. Twenty-one out of 231 BILs were tolerant of low-phosphorus according to the index to P-deficiency tolerance. Twenty-three QTLs on chromosomes 1, 2, 3, 7, 8, 9 and 11 were detected, of which eight QTLs showed high (22.93–37.32%) contribution to phenotypic variation. In addition, most of QTLs in this study (18 out of 23 QTLs) were located and overlapped on the chromosome 1, 3 and 11, which individually explained 6.07–34.70% phenotypic variation, indicating that there might be multiple main effect QTLs related to P-deficiency tolerance in O. rufipogon, and these QTLs might cluster in the same region. These results would provide helpful information for cloning and utilizing the P-deficiency tolerance-responsive genes from O. rufipogon.
KeywordsCommon wild rice P-deficiency tolerance Linkage map QTL Backcross recombinant inbred lines
This research was partially supported by the National Natural Science Foundation of China (31260255, 31360147 and 31660384), the Development Program for Young Scientists of Jiangxi Province, China (20112BCB23007) and the Scientific Planning Project of Jiangxi Provincial Education Department (GJJ12184 and KJLD12059).
- Chen XR, Chen M, He HH, Zhu CL, Peng XS, He XP, Fu JR, Ouyang LJ (2011) Low-phosphorus tolerance and related physiological mechanism of Xieqingzao B//Xieqingzao B/Dongxiang wild rice BC1F9 populations. Chin J Appl Ecol 22:1169–1174Google Scholar
- Heuer S, Lu XC, Joonghyoun C, Tanaka JP, Kanamori H, Matsumoto T, Leon TD, Ulat VJ, Ismail AM, Yano M, Wissuwa M (2009) Comparative sequence analyses of the major quantitative trait locus phosphorus uptake 1 (pup1) reveal a complex genetic structure. Plant Biotechnol J 7(5):456–471CrossRefPubMedGoogle Scholar
- Huang C (2005) Screening for tolerance to low-phosphorus Stress of Chinese japonica rice core collection (Oyrza sativa L.) and detection of QTL for tolerance to low-phosphorus of rice. Dissertation, China Agricultural University, pp 18–25Google Scholar
- Mu P, Huang C, Li JX, Liu LF, Liu UJ, Li ZC (2008) Yield trait variation and QTL mapping in a DH population of rice under phosphorus deficiency. Acta Agron Sin 34:1137–1142Google Scholar
- Wang S, Basten CJ, Zeng ZB (2005) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, RaleighGoogle Scholar
- Yoshida S, Forno DA, Cock JH, Gomez KA (1976) Laboratory manual for physiological studies of rice, 3rd edn. International Rice Research Institute, ManilaGoogle Scholar