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Euphytica

, Volume 207, Issue 2, pp 387–399 | Cite as

Comparison of seven colchicine-induced tetraploid clones with their original diploid clones in purple coneflower (Echinacea purpurea L.)

  • Rong Chen
  • Wei-zhen Jiang
  • Qing-ling Li
  • Xin-lian Li
  • Xiao-lu Chen
  • Yue-sheng Yang
  • Hong Wu
Article
  • 320 Downloads

Abstract

Seven clones of colchicine-induced tetraploid purple coneflower (Echinacea purpurea L.) plants were grown on a farm for comparison with their corresponding original diploid plants. In the first year of cultivation, the plant height, number of capitula, and inflorescence (branched flower stalk) were significantly reduced in all of the tetraploid plants, while the length-to-width leaf ratio, pollen size, and root thickness were significantly increased. Although the tetraploid plants had larger seeds, most of the seeds of tetraploid plants were not fully filled and the naked seeds (i.e., seeds observed without the outer seed coat) were noticeably smaller than those of the diploid plants. In the second year of cultivation, the phenological stages were markedly delayed in the tetraploid plants; the number of capitula increased much more in tetraploid plants than in diploid plants. Among the seven tetraploid clones, two yielded significantly higher plant biomass and higher cichoric acid content per gram dry weight as well as per plant. The results of the present experiments indicate that some effects of tetraploidization of diploid E. purpurea are genotype-dependent, suggesting that the tetraploidization of more genotypes of the diploid is needed to breed better tetraploid varieties.

Keywords

Purple coneflower Tetraploid Cultivation Growth In vitro culture Cichoric acid 

Notes

Acknowledgments

This research was funded by a grant from Science and Technology Planning Project of Guangdong Province, China (2011B031700026).

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Rong Chen
    • 1
    • 2
  • Wei-zhen Jiang
    • 2
  • Qing-ling Li
    • 2
  • Xin-lian Li
    • 2
  • Xiao-lu Chen
    • 2
  • Yue-sheng Yang
    • 2
    • 3
  • Hong Wu
    • 2
    • 3
  1. 1.Key Laboratory of Crops with High Quality and Efficient Cultivation and Security Control, Yunnan Higher Education InstitutionsHonghe UniversityMengziChina
  2. 2.Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Natural MedicineSouth China Agricultural UniversityGuangzhouChina
  3. 3.Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant GermplasmSouth China Agricultural UniversityGuangzhouChina

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