Variability of tocopherols, tocotrienols and avenanthramides contents in European oat germplasm
- 378 Downloads
A broad spectrum of European oat genetic resources representing crop wild relatives, landraces, traditional, obsolete and modern cultivars, was observed in a multi-location field study all over Europe. Here analyses results of 173 accessions for tocopherols and tocotrienols, and of 137 accessions for avenanthramides from groat samples are reported. Genotype, environment and interaction effects, as observed on a set of standard cultivars, were highly significant for most of the targeted traits. Environment effects were often dominant. Higher contents of these compounds than previously reported in oat have been frequently found. High values of α-tocotrienol and avenanthramides were detected in grains of Avena strigosa. Also few cultivars of A. s ativa reached more than 100 mg kg−1 α-tocotrienol. Contents of ß, γ- and δ-tocols were low; the latter often close to the detection limits. Presence of avenanthramides in Avena species other than A. s ativa is shown for the first time.
KeywordsAvena ssp. Avenanthramides Environmental effects Genetic resources Tocols
The authors wish to thank the partners involved in the multiplication, de-hulling and milling of the selected accessions and in the determination of the seed weight: Külli Annamaa, Jõgeva Plant Breeding Institute, Estonia; Izabela Kordulasinska and Zofia Bulinska, IHAR, Radzików, Poland; Nadezhda Antonova, Institute of Plant Genetic Resources “K. Malkov”, Sadovo, Bulgaria; Alf Ceplitis, Svalöf Weibull AB, Svalöv, Sweden; Jean Koenig and Gérard Branlard, INRA, Clermont-Ferrand, France; Danela Murariu, Vegetal Genetic Resources Bank of Suceava, Romania; Matthias H. Herrmann, Julius Kühn Institute, Groβ Lüsewitz, Germany. Janicka Nilsson is acknowledged for performing the avenanthramide analyses. Further they thank the two referees, who carefully went through the text and made valuable suggestions for clarification and improvement. This research was part of the European Project “Avena genetic resources for quality in human consumption”, co-funded by the European Commission (AGRI GENRES 061, council regulation 870/2004) with additional contributions by Peter Koelln KGaA, Elmshorn, Germany, Emco spol. s r. o., Prague, Czech Republic and Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V., Bonn, Germany.
- Dimberg LH, Theander O, Lingnert H (1993) Avenanthramides—a group of phenolic compounds in oats. Cereal Chem 70:637–641Google Scholar
- EFSA Panel on Dietetic Products, Nutrition and Allergies (2010) Scientific opinion on the substantiation of a health claim related to oat beta glucan and lowering blood cholesterol and reduced risk of (coronary) heart disease pursuant to article 14 of regulation (EC) No. 1924/2006. EFSA J 8(12):15Google Scholar
- Irakli MN, Samanidou VF, Papadoyannis IN (2012) Optimization and validation of the reversed-phase high-performance liquid chromatography with fluorescence detection method for the separation of tocopherol and tocotrienol isomers in cereals, employing a novel sorbent material. J Agric Food Chem 60:2076–2082CrossRefGoogle Scholar
- Katsiotis A, Germeier CU, Koenig J, Legget M, Bondo L, Frese L, Bladenopoulos K, Ottoson F, Mavromatis A, Veteläinen M, Menexes G, Drossou A (2009) Screening a European Avena landrace collection using morphological and molecular markers for quality and resistance breeding. In: Molina-Cano JL, Christou P, Graner A, Hammer K, Jouve N, Keller B, Lasa JM, Powell W, Royo C, Shewry P, Stanca AM (eds) Cereal science and technology for feeding ten billion people: genomics era and beyond. Zaragoza: CIHEAM/IRTA (Options Méditerranéennes: Série A. Séminaires Méditerranéens, 81), pp 27–30Google Scholar
- Loskutov IG (1998) Database and taxonomy of VIR’s world collection of the genus Avena L. In: Maggioni L, Leggett M, Bücken S, Lipman E (compilers): Report of a Working Group on Avena: Fifth Meeting, Vilnius, Lithuania, International Plant Genetic Resources Institute, Rome, pp 26–32Google Scholar
- Murariu D, Placinta DD, Germeier CU, Annamaa K, Antonomova N, Bulinska-Radomska Z, Koenig J, Terzi V (2013) Quality characteristics of European Avena genetic resources collections. Rom Agric Res 30:45–50Google Scholar
- Piepho HP (2012) A SAS macro for generating letter displays of pairwise mean comparisons. Commun Biometry Crop Sci 7:4–13Google Scholar
- Pisacane V, Redaelli R, Berardo N (2004) Reducing time analysis for the determination of tocols in cereals by Normal-Phase High Performance Liquid Chromatography. J Genet Breed 58:253–258Google Scholar
- Shewry PR, Piironen V, Lampi A-M, Nyström L, Li L, Rakszegi M, Fraś A, Boros D, Gebruers K, Courtin CM, Delcour JA, Andersson AAM, Dimberg LH, Bedő Z, Ward JL (2008) Phytochemical and fiber components in oat varieties in the HEALTHGRAIN diversity screen. J Agric Food Chem 56:9777–9784CrossRefGoogle Scholar
- The Food and Drug Administration (1997) Food labeling: health claims, oats and coronary heart disease. Final rule. Federal Register 62:3583–3601Google Scholar