Identification of QTLs controlling low-temperature germination of the East European rice (Oryza sativa L.) variety Maratteli
- 535 Downloads
Germinability under low temperature is one of the most important traits in seedling establishment in direct-sowing culture of rice. The objective of this study was the identification of genes responsible for higher and faster germination under low temperature, with the aim of breeding new rice varieties for direct-sowing culture. We identified four quantitative trait loci (QTLs) responsible for germination properties under low temperature in an East European rice variety, Maratteli. Maratteli-derived alleles at qLTG3-1 and qLTG3-2 on chromosome 3 and qLTG11-1 on chromosome 11 increased germination rate under low temperature. In contrast, the Maratteli allele at qLTG1-1 on chromosome 1 reduced the rate. Near-isogenic lines (NILs) with the Akitakomachi genetic background harboring qLTG3-1 and qLTG11-1 alleles from Maratteli accelerated germination and a NIL with qLTG1-1 delayed germination under low temperature, resulting in enhanced or suppressed germination rates, respectively. These QTLs altered the germination profile of Akitakomachi. Hence, Maratteli is a better source of genes for the improvement of germination under low temperature. We conclude that the low temperature germinability of various Japanese varieties inferior in this character can be improved by marker-assisted selection without modification of other superior traits. These NILs may also contribute to uncovering the molecular mechanisms of cold tolerance at the germination stage.
KeywordsRice (Oryza sativa L.) Germinability at low temperature Direct-sowing QTLs NIL Marker-assisted selection
We thank Dr. Hisanori Ohta of National Agriculture and Food Research Organization, Agricultural Research Center, for providing seeds of Maratteli rice. This research was supported by a grant from Akita Prefectural University in the industry-academia joint research program.
- Ikehashi H (1975) Dormancy formation and subsequent changes of germination habits in rice seeds. Japan Agri Res Quar 9:8–12Google Scholar
- Ji SL, Jiang L, Wang YH, Liu SJ, Liu X, Zhai HQ, Yoshimura A, Wan JM (2008) QTL and epistasis for low temperature germinability in rice. Acta Agron Sin 34:551–556Google Scholar
- Matsumoto S, Masaki S, Kawamoto T, Kodama I (2001) Selection by simple method for improvement of the low temperature emergence ability of rice. Tohoku J Crop Sci 44:49–50Google Scholar
- McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res 9:199–207CrossRefGoogle Scholar
- Miura K, Araki H (1996) Low temperature treatment during the imbibition period for the induction of secondary dormancy in rice seeds (Oryza sativa L.). Breed Sci 46:235–239Google Scholar
- Tezuka K, Miyadate H, Kato K, Kodama I, Matsumoto S, Kawamoto T, Masaki S, Satoh H, Yamaguchi M, Sakurai K, Takahashi H, Satoh-Nagasawa N, Watanabe A, Fujimura T, Akagi H (2010) A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice variety Cho-Ko-Koku. Theor Appl Genet 120:1175–1182CrossRefGoogle Scholar
- Wang S, Basten CJ, Zeng ZB (2004) Windows QTL cartographer 2.5. North Carolina State University, RaleighGoogle Scholar
- Yoshida S (1981) Fundamentals of Rice Crop Science. International Rice Research Institute, Los BañosGoogle Scholar