Seed germination and seedling growth of Scots pine in technogenically polluted soils as container media

  • Svetlana Makhniova
  • Pavel Mohnachev
  • Sezgin AyanEmail author


Reforestation of technologically polluted areas has become an increasingly important issue. In this study, seed germination capacity and survival rate and morphometric characteristics of Scots pine (Pinus sylvestris L.) seedlings grown in a magnesite-polluted soil medium were investigated in a pot experiment. Significant differences in seed field germination, sprout survival, seedling length at various growth stages, and root collar diameter of the seedling were discovered between the trial variants for the pot trial using growing media from the polluted areas and the control site. In addition, it was observed that the differences between the trial variants depended on seed origin and the level of soil pollution. The data indicate that seed germination and seedling growth were significantly reduced as the levels of pollution increased. These negative effects of the pollution tend to increase as the seedling gets older.


Pinus sylvestris Technogenic Emission Seedlings Morphometric 



The work was carried out within the framework of the State task of the Botanical Garden of the Ural Branch of the Russian Academy of Sciences. The authors would like to thank Prof. Dr. Oktay YILDIZ, Düzce University, Faculty of Forestry, in Turkey, and Prof. Dr. Sergey MENSHIKOV Botanical Garden Ural Branch of Russian Academy of Sciences, Yekaterinburg, Russia, for their contributions to the paper.


  1. Agrawal, M., & Agrawal, S. B. (1989). Phytomonitoring of air pollution around a thermal power plant. Atmospheric Environment., 23(4), 763–769.CrossRefGoogle Scholar
  2. Anikeev, D. R, Babushkina, L. G., & Zueva, G. V. (2000). The state of the reproductive system of Scotch pine with aerotechnogenic contamination. Yekaterinburg. 81 p.Google Scholar
  3. Anonymous. (1962). Agroclimaticheskiy spravochnik po Sverdlovskoy oblasti (Sverdlovsk Region Agroclimatic Data Sheet)/Ural. Hydrometeorological Service Bureau - Leningrad: Hydrometeoizdat, p. 196 (in Russian).Google Scholar
  4. Anonymous. (1964). Agrokhimicheskie Kharacteristikie pochv SSSR. Rayony Urala (agrochemical characteristic of soils in the USSR. Regions of Ural). Moskva: Nauka, p. 196 (in Russian).Google Scholar
  5. Ayari, A., Meftahi, M., Zammeli, F., & Khouja, M. L. (2016). Seed production variability of Aleppo pine (Pinus halepensis mill.) within Korbus arboretum (north east of Tunisia). Global Journal of Botanical Science., 4, 20–23.CrossRefGoogle Scholar
  6. Bakhtiyarova, R. M., Starova, N. V., & Yanbaev, Y. A. (1995). Genetic changes in populations of Scotch pine growing under industrial air pollution conditions. Silvae Genetica., 44(4), 157–160.Google Scholar
  7. Bender, O. G., Velisevitch, S. N., Tchitorkina, O. Y., Zotikiva, A. P., & Tchernova, N. A. (2012). Analysis of impact of the growing media quality and seeds origin on the Siberian cedar seedlings morphogenesis. Tomsk State University Bulletin. Biology, 1(17), 109–121.Google Scholar
  8. Chibrik, T. S., Lukina, N. V., Filimonova, Y. I., & Glazyrina, M. A. (2012). The structure and dynamics of forest рhytocoenosis on the disturbed industrial lands. Izvestia of Samara Scientific Center of the Russian Academy of Sciences, 14. 1(5), 1403–1406.Google Scholar
  9. Chropenova, M., Greguskova, E. K., Karaskovaa, P., Pribylova, P., Kukucka, P., Barakovaa, D., & Cupr, P. (2016). Pine needles and pollen grains of Pinus mugo (Turra.)—a biomonitoring tool in High Mountain habitats identifying environmental contamination. Ecological Indicators, 66, 132–142.CrossRefGoogle Scholar
  10. Fedorkov, A. L. (2007). Adaptation of coniferous species to the boreal climate of northern Europe. Lesovedenie., 3, 46–51.Google Scholar
  11. Geraskin, S. A., Dikareva, N. S., Udalova, A. A., Spiridonov, S. I., & Dikarev, V. G. (2008). Cytogenetic effects in populations of pine in ordinary from Bryan region areas exposed to radioactive contamination as a result of an accident at Chernobyl NPP. Radiation Biology. Radioecology., 48(5), 584–595.Google Scholar
  12. Gluschenko, N. N., & Olkhovskaya, I. P. (2014). Ecological safety in energetics. Characteristics of fly ash particles from coal power plants. Proceedings of the Russian Academy of Sciences. Power Engineering Journal, 1, 20–28.Google Scholar
  13. Hisamoto, Y., & Goto, S. (2017). Genetic control of altitudinal variation on female reproduction in Abies sachalinensis revealed by a crossing experiment. Journal of Forest Research., 22(3), 195–198.CrossRefGoogle Scholar
  14. Ivanov, Y. V., Savochkin, Y. V., Shumeiko, E. V., & Kuznetsov, V. V. (2013). Implementation of the early stage of pine ontogenesis of the orded oriented on the background of toxic concentrations of copper ions. Bulletin of Tomsk State University. Biology, 1(21), 103–117.Google Scholar
  15. Kalashnik, N. A., Yasovieva, S. М., & Presnukhina, L. P. (2008). Pine trees pollen abnormalities under the conditions of industrial pollution in the South Ural. Forest Science., 2, 33–40.Google Scholar
  16. Kalchenko, V. A., Arkhipov, N. P., & Fedotov, I. S. (1993). Mutagenesis of enzyme loci, induced in megaspores of Pinus sylvestris L. by ionizing radiation from the accident at the Chernobyl atomic power plant. Russian Journal of Genetics, 29, 266.Google Scholar
  17. Kizilshtein, L. Y., & Levchenko, S. V. (2003). Impurity elements and environmental problems of coal power engineering. Thermal Engineering., 50(12), 981–986 (in Russia).Google Scholar
  18. Kolesnikov, B. P. (1969). Forests of Chelyabinsk region. Forests of the USSR. Moscow, 46, 125–157 (in Russian).Google Scholar
  19. Kolesnikov, B. P., Zubareva, R. S., & Smologonov, E. P. (1973). Lesorastitelinye uslovia I tipy lesov Sverdlovskoy oblasti: practicheskoe rukovodstvo (Forest site and types of forest in Sverdlovsk region: practical guide). Sverlovsk: STC The USSR AS, p. 176 (in Russian).Google Scholar
  20. Korshikov, I. I., Lapteva, H. V., & Belonozhko, Y. A. (2015). Quality of pollen and cytogenetic changes of Scotch pine as indicators of the impact of the technogenically polluted environment of Krivoy Rog. Contemporary Problems of Ecology., 8(2), 250–255.CrossRefGoogle Scholar
  21. Lapirov, A. G., & Lebedeva, O. A. (2009). Influence of nitrate salts of some heavy metals on the initial stages of ontogenesis of Batrachium trichophyllum (Chaix) Bosch. Bulletin of Tomsk State University, 323, 364–369.Google Scholar
  22. Lyanguzova, I. V. (2011). Effect of industrial air pollution on wild plant seed germination and seedling growth. Russian Journal of Plant Physiology., 58(6), 991–998.CrossRefGoogle Scholar
  23. Lyanguzova, I. V. (2017). Dynamic trends of heavy metal contents in plants and soil under different industrial air pollution regimes. Russian Journal of Ecology., 48(4), 311–320.CrossRefGoogle Scholar
  24. Makhniova, S. G. (2016). Quality pollen of scotch pine under the conditions of technogenic pollution Reft power plant. The forests of Russia and the economy in them., 4(59), 55–62.Google Scholar
  25. Makhniova, S. G., & Mohnachev, P. E. (2014). Quality of seed posterities of the Scotch pine of different origins on the levelled ecological background. In Forest biogeocenoses of the Boreal zone: geography, structure, functions, and dynamics (pp. 343–346). Novosibirsk: Publishing house of the Siberian Branch of the Russian Academy of Sciences (in Russian).Google Scholar
  26. Makhniova, S. G., Babushkina, L. G., & Zueva, G. V. (2003). Condition of Scotch pine male generative sphere under the environment of technogenic pollution. Yekaterinburg: USFEA, Ural University Publishing House.Google Scholar
  27. Makhniova, S. G., Mohnachev, P. E., & Menshikov, S. L. (2013). Influence of soil conditions and the origin of seeds of the scotch pine on their laboratory and soil viability. Notifications of Orenburg State Agrarian University, 3(41), 10–12 (in Russian).Google Scholar
  28. Makhniova, S. G., Kuzmina, N. A., & Menshikov, S. L. (2017). Quality of Scotch pine pollen depending on the aerotechnogenic pollution level with emissions from Reftinskiy GRES power plant. Journal of Geoscience and Environment Protection., 5, 99–117. Scholar
  29. Mendoza, R. E., García, I. V., Cabo, L., Weigandt, C. F., & Iorio, A. F. (2015). The interaction of heavy metals and nutrients present in soil and native plants with arbuscular mycorrhizae on the riverside in the Matanza-Riachuelo river basin (Argentina). Science of the Total Environment., 505, 555–564.CrossRefGoogle Scholar
  30. Menshikov, S. L. (1985). Investigation of ecological peculiarities of growth and rationale of agro-technologies for creation of coniferous cultures in conditions of magnetic properties (Doct. Diss). Sverdlovsk. 210 p. (in Russian).Google Scholar
  31. Menshikov, S. L., & Ivshin, A. P. (2006). Forest-tundra and taiga transformation regularities under the conditions of technogenic pollution. Yekaterinburg: UB RAS.Google Scholar
  32. Menshikov, S. L., Srodnykh, T. B., & Terekhov, G. G. (1987). Peculiarities of the chemistry of soils and anatomic-morphological structure of the assimilation apparatus of pines and birch under the conditions of magnesite dust. Russian Journal of Ecology., 5, 84–87 (in Russian).Google Scholar
  33. Menshikov, S. L., Kuzmina, N. A., & Mohnachev, P. E. (2012). Magnesite emissions impact on the soils and snow blanket // Orenburg State Agrarian University. Review, 5(37), 221–224.Google Scholar
  34. Menshikov, S. L., Zavyalov, K. E., Kuzmina, N. A., Mokhnachev, P. E., & Tsepordey, I. S. (2016). Distribution of Betula pendula Roth. test crops by diameter class and level of pollution of soil in the area of emissions of the JSC «Magnesite» integrated industrial complex. Advances in current natural sciences., 10, 84–89.Google Scholar
  35. Micieta, K., & Murin, G. (1997). The use of Pinus sylvestris L. and Pinus nigra Arnold as bio indicator species for environmental pollution. Cytogenetic studies of forest trees and shrub species. Zagreb: 253–264.Google Scholar
  36. Mikhailova, T. A., & Shergina, O. V. (2011). Nutritional status of woody plants as an integral indicator of the state of urban ecosystem. Proceedings of the Irkutsk state University, Series Biology. Ecology, 4(2), 66–73 (in Russian).Google Scholar
  37. Mikhailova, T. A., Berezhnaya, N. S., Ignatieva, O. V., & Afanasieva, L. V. (2003). Alteration of the element balance in Scotch pine needles under industrial pollution. Contemporary Problems of Ecology., 6, 755–762.Google Scholar
  38. Mikhailova, T. A., Shergina, O. V., & Berezhnaya, N. S. (2007). Biogeochemical redistribution of industry-caused sulphur in an urban ecosystem. Chemistry for Sustainable Development., 15(3), 351–358.Google Scholar
  39. Mikhailova, T. A., Shergina, O. V., & Kulagina, O. V. (2015). Indicative indicators of violation of forest ecosystems technogenic pollution. International Journal of Applied and Fundamental Research., 2, 78–82.Google Scholar
  40. Minkina, T. M., Motuzova, G. V., Miroshnichenko, N. N., Fateev, A. I., Mandzhieva, S. S., & Chaplygin, V. A. (2013). Accumulation and distribution of heavy metals in plants in the technogenesis zone. Agricultural Chemistry., 9, 65–75 (in Russian).Google Scholar
  41. Mityakova, I. I. (2012). Soil-ecological conditions impact on the Scotch pine seedlings vegetation. Kuban State Agrarian University Scientific Journal., 81(7), 2–12.Google Scholar
  42. Mohnachev, P. E. (2014). Female generative sphere of the Scotch pine in the conditions of magnesite pollution. Materials of the All-Russian scientific conference with the international participation devoted to the 70 anniversary of creation of V. N. Sukachyov Institute of Forests of the Siberian Branch of the Russian Academy of Sciences: Forest biogeocenoses of the Boreal zone: geography, structure, functions, dynamics, Krasnoyarsk, 2014 (pp. 348–351). Novosibirsk: Publishing house of the Siberian Branch of the Russian Academy of Sciences.Google Scholar
  43. Mohnachev, P. E., Makhniova, S. G., & Menshikov, S. L. (2013). Features of reproduction of the Scotch pine (Pinus silvestris L.) in the conditions of pollution by magnesite dust. Notifications of Orenburg State Agrarian University., 3(41), 8–9 (in Russian).Google Scholar
  44. Mohnachev, P. E., Makhniova, S. G., Menschikov, S. L., Zavyalov, K. E., Kuzmina, N. A., & Potapenko, A. M. (2016). Sowing qualities of seeds Scotch pine in the emissions anthropogenic magnesite production. The forests of Russia and the economy in them., 4(59), 42–48.Google Scholar
  45. Muratova, E. N., & Zubareva, O. N. (1990). Cytogenetic study of Pinus sylvestris L. in the district of thermal power plant emissions. Proceedings of the USSR Academy of Sciences. The biological series, 3, 36.Google Scholar
  46. Nekrasova, T. P. (1983). Pollen and pollen behavior of Siberian conifers. Novosibirsk: Nauka 186 p. (in Russian).Google Scholar
  47. Pimenov, A. V., Sedelnikova, T. S., & Yefremov, S. P. (2014). Morphology and quality of the Scotch pine pollen in contrasting ecotopes of Khakassia. Lesovedenie., 1, 57–64.Google Scholar
  48. Pukkala, T., Hakkanen, T., & Nikkanen, T. (2010). Prediction model for the annual seed crop of Norway spruce and Scotch pine in Finland. Silva Fennica, 44(4), 629–642.CrossRefGoogle Scholar
  49. Reshetova, S. A., Solodukhina, M. A., & Yurgenson, G. A. (2015). The interrelation between pollen abnormalities and polymorphism and the increased contents of toxic elements in flowers and flower buds in Aconogonon angustifolium (Pall.) Hara. and Papaver nudicaule L. Russian Journal of Ecology., 46, 36–42. Scholar
  50. Romanovsky, M. G. (1997). Formation of the pine seed yield is normal and with mutagenic contamination. Moscow: Nauka 112 p.Google Scholar
  51. Sadakova, K. A., & Kolyasnikova, N. L. (2014). Pollen grains fertility and heavy metals content Scotch pine pollen growing in the areas with different anthropogenic load. Contemporary Problems of Science and Education., 6, 1–14.Google Scholar
  52. Sazonova, T. A., Bolondinskiy, V. K., & Pridacha, V. B. (2011). Scotch pine ecological-phisiological characteristics. Petrozavodsk.Google Scholar
  53. Seregin, I. V., & Ivanov, V. B. (2001). Physiological aspects of cadmium and lead toxic effects on higher plants. Russian Journal of Plant Physiology., 48(4), 523–544.CrossRefGoogle Scholar
  54. Skripalschikova, L. N., Dneprovskiy, I. A., Stasova, V. V., Pliashechnik, M. A., Greshilova, N. V., & Kalugina, O. V. (2016). Scotch pine acerose leaf morphology-anatomic peculiarities under the influence of Krasnoyarsk city industrial emissions. Siberian Journal of Forest Science., 3, 46–56.Google Scholar
  55. Srodnykh, T. B., & Menshchikov, S. L. (1992). Growth of forest crops in conditions of pollution by magnesite dust. Technogenic impact on forest communities and the problem of their rehabilitation and conservation (87–92 p.) (in Russia).Google Scholar
  56. Tretyakova, I. N., & Noskova, N. E. (2004). Scotch pine pollen under the conditions of ecological stress. Ecology, 1, 26–34.Google Scholar
  57. Trowbridge, P. J., & Bassuk, N. L. (2004). Trees in the urban landscape: site assessment, design, and installation. Hoboken: John Wiley & Sons, Inc. 232 p.Google Scholar
  58. Vasilevskaya, N. V., & Petrova, N. V. (2014). Pollen morphological variability Pinus sylvestris L. under the conditions of city (case study Monchegorsk City). Bulletin of Petrazavodsk State University., 4, 7–12.Google Scholar
  59. Velisevich, S. N. (2017). Pollen quality of Pinus sibirica Du Tour (Pinaceae) mountain populations in arid and humid regions of Altai. Journal of Siberian Federal University. Biology, 10(3), 301–311.CrossRefGoogle Scholar
  60. Vodyanitsky, Y. N., Smagin, A. V., & Yakovlev, A. S. (2016). Variation factors of mobile forms of heavy metals in soil. The North Caucasus Ecological Herald., 1, 27–38 (in Russian).Google Scholar
  61. Yerkoeva, A. A., Drozdov, S. N., & Holoptseva, E. S. (2012). Acidity planting media impact on the ecophysiological characteristics of the Scotch pine seedlings. Materials of Karelian Scientific Centre RAS, No. 2. pp. 84–90.Google Scholar
  62. Zavyalov, K. E. (2013). Morphology and chemical composition of leaves of pilot cultures of the silver birch (Betula pendula Roth) in the conditions of magnesite pollution. Izvestia of Orenburg State Agrarian University., 3(41), 230–232 (in Russian).Google Scholar
  63. Zavyalov, K. E., & Menshikov, S. L. (2009). Condition of birch cultures in the conditions of magnesite pollution (pp. 60–61). Agricultural Russia. Special issue (in Russian).Google Scholar
  64. Zavyalov, K. E., & Menshikov, S. L. (2010). Over ground phytomass of pilot cultures of the birch in conditions of magnesite dust pollution. Izvestia of Orenburg State Agrarian University., 4(28), 27–30 (in Russian).Google Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Svetlana Makhniova
    • 1
    • 2
  • Pavel Mohnachev
    • 1
  • Sezgin Ayan
    • 3
    Email author
  1. 1.Botanical Garden Ural Branch of Russian Academy of SciencesYekaterinburgRussia
  2. 2.Russian State Vocational Pedagogical UniversityYekaterinburgRussia
  3. 3.Faculty of Forestry, Silviculture DepartmentKastamonu UniversityKastamonuTurkey

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