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Coal mine effluent-led bioaccumulation of heavy metals and histopathological changes in some tissues of the catfish Clarias batrachus

  • Kalpana C. Lakra
  • Bechan Lal
  • Tarun Kumar BanerjeeEmail author
Article
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Abstract

Coal mining generates huge quantity of toxic effluent which consistently pollutes the neighboring wetlands where the local inhabitants regularly cultivate edible fishes. In the present study the concentration of heavy metals Fe, Zn, Cu, Mn, Ni, Cd, Pb and Cr were analyzed in the water and various tissues of edible catfish Clarias batrachus reared in a pond receiving effluents from Rajrappa coal mine, Jharkhand, India. The metal concentrations in the pond water were dramatically higher (Fe 350%, Zn 423%, Cu 12%, Mn 7029%, Ni 713%, Cd 1700%, Pb 4333% and Cr 588%) than the safe limit of Environmental Pollution Agency (2003) as well as the control tap water. Excessive amounts of metals in effluent caused their substantial transfer to the different tissues of the catfish reared in such ponds. Results showed that accumulation of metals in fish tissues were in the following order: liver > kidney > air breathing organ (ABO) > gills > skin > brain > muscles. Among the various tissues the highest accumulation of most of the metals was recorded in the liver (2.05–271.28 mg/kg dry weight) and lowest in the muscles (1.39–30.27 mg/kg dry weight), while the concentration of metals in other tissues ranged in between. The accumulation of heavy metals in tissues appears to cause remarkable histopathological alterations in skin, gills, ABO, liver and kidney that might be leading to deleterious effect on fish physiology and consequently impact the consumers of such fishes.

Keywords

Bioaccumulation Clarias batrachus Coal mine effluent Heavy metal Histopathological alterations Pond water 

Notes

Acknowledgements

Thanks are due to Authorities of Central Coalfields Limited, Jharkhand for collaboration. Authors also acknowledge the help of Prof. B. R. Maurya, Head, Department of Soil Science and Agricultural Chemistry for providing atomic absorption spectrophotometer (AAS) facility.

Funding information

K. C. Lakra was supported as Junior Research Fellow and Senior Research Fellow under the Maulana Azad National fellowship (vide letter no.: F1-17.1/2014-15/MANF-2014-15-CHR-JHA-32885) scheme. This work received financial support from University Grant Commission, New Delhi in the form of a Major Research Project (P-01/694, 42-529/2013 SR).

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Kalpana C. Lakra
    • 1
  • Bechan Lal
    • 1
  • Tarun Kumar Banerjee
    • 1
    Email author
  1. 1.Centre of Advanced Study, Department of Zoology, Institute of ScienceBanaras Hindu UniversityVaranasiIndia

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