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Complete genomic sequence of Noni mosaic virus (NoMV) associated with a mosaic disease in Morinda citrifolia L.

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Abstract

An outbreak of a virus-like disease has caused severe damage to noni plants (Morinda citrifolia L.) in Xishuangbanna area of Yunnan province in southwest China since 2015. The diseased plants displayed typical mosaic symptom with light and dark green patches on leaves. Flexuous filamentous virus particles of about 800 nm in length were observed from the leaf saps by transmission electron microscope. Illuemina transcriptomic sequencing further revealed the presence of a potyvirus and its near complete genome was obtained from de novo assembly. The complete genome of 9659 nts was obtained by Sanger sequencing of eight amplicons generate by RT-PCR and 5′ and 3’ RACE. BLASTp analysis of the polyprotein sequence showed that the virus was most closely related to Tobacco vein banding mosaic virus (TVBMV), but these two viruses only shared 50.7% amino acid sequence similarity. Both phylogenetic analyses of the polyprotein and CP amino acid sequences indicated that this virus is a member of genus Potyvirus. However, the low sequence homology with all known potyviruses established this virus as a new species in the genus, tentatively named as Noni mosaic virus (NoMV). Our field surveys showed that 100% of the symptomatic samples and 28.57% of the asymptomatic samples were infected with this novel potyvirus. Aphids collected from diseased leaves were also detected carrying the virus and aphid transmission test confirmed it can transmit the NoMV. In summary, our data indicated that a novel species of potyvirus, NoMV, is prevalent in Yunnan, China and is associated with an emerging mosaic disease on M. citrifolia.

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Funding

This study was funded by the Major Science and Technology Program of Hainan Province (grant number ZDKJ2017003), Young Elite Scientists Sponsorship Program by CSTC (No. CSTC-QN201704) and Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences (No. 2017B030314113).

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Correspondence to Nai-Tong Yu or Yan Yang or Zhi-Xin Liu.

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The authors declare no conflict of interest.

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This manuscript did not involve any human participants, and/ or animals.

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Yu, N., Cai, Z., Xiong, Z. et al. Complete genomic sequence of Noni mosaic virus (NoMV) associated with a mosaic disease in Morinda citrifolia L.. Eur J Plant Pathol (2020). https://doi.org/10.1007/s10658-020-01948-4

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Keywords

  • Noni mosaic virus
  • Potyvirus
  • Complete genome
  • Morinda citrifolia L
  • Illumina transcriptomic sequencing