Molecular characterization of Alfalfa mosaic virus (AMV) isolates in alfalfa and other plant species in different regions in Saudi Arabia
- 47 Downloads
In a survey conducted in 2012 and 2013, 1166 samples from alfalfa plants and 202 samples from symptomatic weeds and cultivated plants growing adjacent to alfalfa fields were collected. Using DAS-ELISA, Alfalfa mosaic virus (AMV) was detected in 58.4% of the alfalfa samples and in 63.9% of the weeds and cultivated plants samples. ELISA detection of AMV was confirmed by testing representative samples from all regions by RT-PCR using a pair of primers (AMV/F and AMV/R) specific to the AMV coat protein (CP) gene. The size of the major product obtained from AMV-infected plants was identical to the 700 bp size expected from the CP gene of AMV. The amino acids and the nucleotide sequences of 17 Saudi AMV isolates from alfalfa, and 16 from other cultivated plants and weeds detected in different regions in Saudi Arabia showed a percentage of similarity that ranged between 87.9%–100% among them and 86.2% - 100% when compared with sequences of 15 different AMV isolates reported in the GenBank. This is the first time AMV was detected in Vigna unguiculata and in the following weed plant species: Chenopodium quinoa, Convolvulus arvensis, Malva parviflora, Hibiscus spp., Hippuris vulgaris, Cichorium intybus, and Flaveria trinervia in Saudi Arabia.
KeywordsAlfalfa AMV ELISA RT-PCR Saudi Arabia
This Project was funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia, Award Number (10-BIO 979-02).
Compliance with ethical standards
This research is part of a project funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia, Grant Number (10-BIO 979-02). This manuscript complies with the Ethical Rules applicable for this journal. The authors declare that they have no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors. Informed consent was obtained from all individual participants included in the study. It also complies with all details of the relevant ethical rules that came under the following headings:
- Ethical responsibilities of authors.
- Compliance with ethical standards.
- Disclosure of potential conflicts of interest.
- Research involving human participants or and/or animals.
- Informed consent.
- Springer’s guide on publishing ethics.
- Fighting plagiarism, piracy and fraud.
It also complies with any other ethical rules required by the journal that may have not been included in this statement.
- Al-Abrahim, J. S. (2014). Molecular identification of Alfalfa mosaic virus isolated from naturally infected alfalfa (Medicago sativa) crop in Saudi Arabia. International Journal of Plant, Animal and Environmental Sciences, 4(1), 348–352.Google Scholar
- AL-Shahwan, I. M. (2002). Alfalfa mosaic virus (AMV) on alfalfa (Medicago sativa L.) in Saudi Arabia. Assiut Journal of Agricultural Sciences, 33, 21–30.Google Scholar
- AL-Shahwan, I. M. (2003). Host index and status of plant viruses and virus-like disease agents in Saudi Arabia. Res. Bult., No.121: Agric. Res. Center, King Saud Univ., pp: 5–27.Google Scholar
- AL-Shahwan, I. M., & Abdalla, O. A. (1998). Identification of Alfalfa mosaic virus (AMV) and other viruses from wild and cultivated plant species and reaction of the available potato cultivars to AMV in Saudi Arabia. Saudi J. Biol. Sci., 5, 39–44.Google Scholar
- Al-Shahwan, I. M., Anaam, A. M., Abdalla, O. A. (2007). Evaluation of greenhouse-grown pepper cultivars to infection with an isolate of Alfalfa mosaic virus (AMV) in Saudi Arabia. Research bulletin No. 151. Agric. Research Center, College of Food and Agricultural Sciences, King Saud University, Kingdom of Saudi Arabia, pp. 5-28.Google Scholar
- AL-Shahwan, I. M., Abdalla, O. A., & AL brahim, J. S. (2010). Effect of Alfalfa mosaic virus (AMV) on the yield of alfalfa (Medicago sativa L.) Cultivars grown in Saudi Arabia. Journal of Agriculture and Veterinary Sciences, Qassim University, 3, 23–36.Google Scholar
- AL-Shahwan, I. M., Al-Saleh, A. M., Abdalla, O. A., Amer, M. A. (2013). Preliminary data on viruses affecting alfalfa in Saudi Arabia. Presented at “International Conference of Plant Disease and Resistance mechanism” held in Vienna on 20–22 February, 2013.Google Scholar
- AL-Shahwan, I. M., Al-Saleh, A. M., Abdalla, O. A., Amer, M. A. (2014). Viruses associated with alfalfa and adjacent weeds and cultivated plants in the Kingdom of Saudi Arabia. Presented at "11thConference of the European Foundation for Plant Pathology" held in Krakow, Poland on 8-13 September, 2014.Google Scholar
- Bancroft, J. B., Moorhead, E. L., Tuite, J., & Liu, H. P. (1960). The antigenic characteristics and the relationship among strains of Alfalfa mosaic virus. Phytopathology, 50, 34–39.Google Scholar
- Forster, R. L. S., Morris-Krsinich, B. A. M., & Musgrave, D. R. (1985). Incidence of alfalfa mosaic virus, lucerne Australian latent virus, and lucerne transient streak virus in lucerne crops in the North Island of New Zealand. New Zealand Journal of Crop and Horticultural Science, 28, 279–282.Google Scholar
- Guy, P. L., & Forster, R. L. S. (1996). Viruses of New Zealand pasture grasses and legumes.: Pasture and forage crop pathology., In S. Chakraborty, K. T. Leath, R. A. Skipp, G. A. Pederson, R. A. bray, G. C. M. Latch, and F. W. Nutter (ed.), pp. 289–302. American Society of Agronomy & the Crop Science Society of America: Madison WI, USA.Google Scholar
- Hall, T. A. (1999). Bio edit: A user-friendly biological sequence alignment editor and analysis program from windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.Google Scholar
- Hiruki, C., Hampton, R. O. (1990). Alfalfa mosaic. In: Stuteville, D. L. and Erwin, D. C., 2nd Ed., APS press, St. Paul "Compendium of Alfalfa Diseases", pp. 54.Google Scholar
- Jaspars, E. M., & Bos, L. (1980). Alfalfa mosaic virus, No. 229 in: Descriptions of plant viruses. England: Commonwealth Mycology Institute Association. Applied Biology. Kew.Google Scholar
- Massumi, H., Maddahian, M., Heydarnejad, J., Hosseini-Pour, A., & Farahmand, A. (2012). Incidence of viruses infecting alfalfa in the southeast and central regions of Iran. Journal of Agricultural Science Technology, 14, 1141–1148.Google Scholar
- Mih, A. M., & Hanson, J. (1998). Alfalfa mosaic virus: Occurrence and variation among isolates from forage legumes in Ethiopia. Tropical Grasslands, 32, 118–123.Google Scholar
- Milbrath, J. A., & McWhorter, F. P. (1953). The reaction of tomato varieties to various strains of Alfalfa mosaic virus. Phytopathology, 43, 479–479.Google Scholar
- Milbrath, J. A., & McWhorter, F. P. (1954). Response of cowpea varieties to strains of Alfalfa mosaic virus. Phytopathology, 44, 498–498.Google Scholar
- Ministry of Agriculture, (2014). Agricultural statistical year book. Volume 24, Saudi Arabia.Google Scholar
- Mughal, S. M., Zadjali, A. D., & Matrooshi, A. R. (2003). Occurrence, distribution and some properties of alfalfa mosaic alfamovirus in the Sultanate of Oman. Pakistan Journal of Agricultural Sciences, 40, 67–73.Google Scholar
- Ormeño, J., Sepúlveda, P., Rojas, R., & Araya, J. E. (2006). Datura genus weeds as an epidemiological factor of Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), and Potato virus Y (PVY) on Solanaceous crops. Agricultura Técnica (Chile), 66, 333–341.Google Scholar
- Parrella, G., Lanave, C., Marchoux, G., Sialer, M. M., Di Franco, A., & Gallitelli, D. (2000). Evidence for two distinct subgroups of Alfalfa mosaic virus (AMV) from France and Italy and their relationships with other AMV strains. Archives of Virology, 145, 2659–2667.PubMedCrossRefPubMedCentralGoogle Scholar
- Sambrook, J., & Russel, D. (2001). Molecular cloning: A laboratory manual. 3rd Ed. Volume 1. Cold Spring Harbor Laboratory Press, New York – USA.Google Scholar
- Šutic, D. D., Ford, R. E., & Tošic, M. T. (1999). Handbook of plant virus diseases. Washington: CRC Press.Google Scholar