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Glasshouse-specific occurrence of basal rot pathogens and the seasonal shift of Rhizoctonia solani anastomosis groups in lettuce

  • Jolien Claerbout
  • An Decombel
  • Anneleen Volckaert
  • Sofie Venneman
  • Isabel Vandevelde
  • Peter Bleyaert
  • Jenny Neukermans
  • Nicole Viaene
  • Monica HöfteEmail author
Original Article
  • 19 Downloads

Abstract

Basal rot is a common disease in Belgian lettuce, which is mainly controlled by fungicides and chemical soil disinfestation. A seasonal appearance of the basal rot pathogens: Rhizoctonia solani, Sclerotinia spp., Botrytis cinerea and Pythium spp. has been reported, but lettuce growers use standard spraying schemes, irrespective of the occurrence of the pathogen. Due to stricter regulations and environmental concerns the superfluous use of fungicides should be omitted. We investigated if the use of fungicides could be reduced by only controlling the active pathogens. Therefore, lettuce was continuously grown in three glasshouses without any fungal disease control and the active pathogens causing basal rot were identified. The occurrence of basal rot pathogens appeared to be glasshouse specific and the different basal rot pathogens were active throughout the year. However, a seasonal appearance of R. solani anastomosis groups and Pythium spp. was observed with AG4-HGI and Pythium ultimum active at higher temperatures and AG2–1, AG-BI, AG1-IB and Pythium sylvaticum at lower temperatures. We report for the first time the isolation of AG-BI from infected plants. Each R. solani anastomosis group had its own optimal growth rate in vitro. Differences in pathogenicity between R. solani anastomosis groups were observed on detached leaves. AG1-IB and AG4-HGI were most pathogenic, followed by AG2–1 and AG-BI. These results show that the fungicide spraying scheme should be adapted to the occurring pathogens in the glasshouse. This information is of high importance in developing a sustainable control strategy for basal rot pathogens.

Keywords

AG-BI Lactuca sativa Temperature dependence Pythium Botrytis cinerea Sclerotinia 

Notes

Acknowledgements

The authors wish to thank Ilse Delaere for her technical assistance. This research was funded by grant no. 140984 from the ‘Flanders Innovation & Entrepeneurship (VLAIO)’.

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest (financial or non-financial).

Research involving human participants and/or animals

This research does not involve human participants and/or animals.

Informed consent

Not necessary, the research does not involve human participants

Supplementary material

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Jolien Claerbout
    • 1
    • 2
  • An Decombel
    • 3
  • Anneleen Volckaert
    • 4
  • Sofie Venneman
    • 5
  • Isabel Vandevelde
    • 5
  • Peter Bleyaert
    • 3
  • Jenny Neukermans
    • 4
  • Nicole Viaene
    • 2
    • 6
  • Monica Höfte
    • 1
    Email author
  1. 1.Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience EngineeringGhent UniversityGhentBelgium
  2. 2.Plant, ILVO (Flanders research institute for agriculture, fisheries and food)MerelbekeBelgium
  3. 3.Inagro vzwRumbeke-BeitemBelgium
  4. 4.PCG (Provinciaal Proefcentrum voor de Groenteteelt Oost-Vlaanderen vzw)KruishoutemBelgium
  5. 5.PSKW (Proefstation voor de groenteteelt)Sint-Katelijne-WaverBelgium
  6. 6.Faculty of SciencesGhent UniversityGhentBelgium

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