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Early transcriptional response of seedling roots to Ralstonia solanacearum in tobacco (Nicotiana tabacum L.)

  • Wenxia Gao
  • Renjie Chen
  • Mingming Pan
  • Weiqi Tang
  • Tao Lan
  • Likun Huang
  • Wenchao Chi
  • Weiren WuEmail author
Article

Abstract

Bacterial wilt (BW) caused by Ralstonia solanacearum (Ras) is a very destructive disease in solanaceous crops. The molecular mechanism underlying BW resistance in solanaceous plants is still unclear. Using RNA sequencing, we investigated the transcriptional response in the seedling roots of a BW-resistant cultivar D101 and a BW-susceptible cultivar Changbohuang (CBH) of tobacco at 3 h after inoculation with Ras. In total, 158 and 835 differentially expressed genes (DEGs) were detected in D101 and CBH, respectively. Only 41 DEGs were in common between the two cultivars. The number of DEGs in D101 was much smaller than that in CBH, suggesting that D101 was less affected by the inoculation than CBH because of its higher resistance, which could be expressed at the early stages of Ras infection. Transcriptome analysis revealed that in D101 two sets of upregulated genes were significantly enriched in two corresponding groups of gene ontology terms regarding glutathione and flavonoids metabolisms, respectively, suggesting that glutathione and flavonoids are probably two types of main substances conferring the early resistance against Ras infection in tobacco root. Our findings provide a valuable clue to deeply investigate the molecular mechanism of BW resistance in tobacco and solanaceous plants.

Keywords

Bacterial wilt Ralstonia solanacearum Tobacco Transcriptional profiling RNA sequencing 

Notes

Compliance with ethical standards

The paper has not been submitted elsewhere for publication, in whole or in part.

Conflict of interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Human and animal studies

The research did not involve any human participants and/or animals.

Informed consent

Informed consent was obtained from all individual participants included in this study.

Supplementary material

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  1. 1.Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of EducationFujian Agriculture and Forestry UniversityFuzhouChina
  2. 2.Fujian Key Laboratory of Crop Breeding by DesignFujian Agriculture and Forestry UniversityFuzhouChina

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