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Morpho-molecular characterization of Ditylenchus gigas and D. oncogenus parasitizing broad bean, Vicia faba, in Algeria

  • Ines Saadi
  • Francesca De LucaEmail author
  • Elena Fanelli
  • Alberto Troccoli
  • Nicola Greco
  • Halima BenbouzaEmail author
Article

Abstract

A nematode survey conducted in 2013 in Algeria, revealed the presence of a normal and giant races of D. dipsaci species complex on broad bean, Vicia faba L. In Algeria, the stem and bulb nematode causes severe economic damage; however, no studies have been conducted for accurate identification of the species and races of nematode infecting faba bean fields, which make management strategies, quarantine measures and phytosanitary actions less efficient. The present study describes the morphological and molecular analyses of two stem nematode populations from Biskra and Guelma regions in Algeria. The results revealed the presence of D. gigas and D. oncogenus in Algeria. The latest one is identified for the first time on Vicia faba as new host plant. The morphology of both populations is similar to other D. gigas and D. oncogenus populations from southern Italy. Molecular phylogeny by using the ITS-5.8S-ITS2 region clearly supported the presence of D. gigas and D. oncogenus in Algeria.

Keywords

Algeria Ditylenchus gigas Ditylenchus oncogenus Vicia faba Morphometrics PCR –RFLP Sequencing 

Notes

Acknowledgements

The Algerian Ministry of Higher Education and Scientific Research has supported part of this research and fellowship for the first author to work at the Istituto per la Protezione Sostenibile delle Piante.

Compliance with ethical standards

The authors declare that the work is in compliance with ethical standards of the journal.

Competing financial interests

The authors declare no competing financial interests.

Conflict of interest

The authors declare no conflict of interests and give their consent for publishing the results of the present study.

Research involving human participants and/or animals

The authors declare that the manuscript does not contain research involving Human Participants and/or Animals.

Supplementary material

10658_2019_1784_MOESM1_ESM.docx (12 kb)
ESM 1 (DOCX 12.2 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Ines Saadi
    • 1
  • Francesca De Luca
    • 2
    Email author
  • Elena Fanelli
    • 2
  • Alberto Troccoli
    • 2
  • Nicola Greco
    • 2
  • Halima Benbouza
    • 3
    Email author
  1. 1.Department of AgronomyBiskra UniversityBiskraAlgeria
  2. 2.Bari, Consiglio Nazionale delle Ricerche (CNR)Istituto per la Protezione Sostenibile delle PianteBariItaly
  3. 3.Institute of Veterinary Sciences and Agronomic Sciences, Department of AgronomyUniversity of Batna-1BatnaAlgeria

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