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European Journal of Plant Pathology

, Volume 155, Issue 1, pp 369–376 | Cite as

Studies on molecular variability of coat protein gene of Papaya ringspot virus-P isolates from India

  • Rajesh KumarEmail author
  • Ashish Srivastava
  • Aparana Srivastava
  • Shalini Srivastava
  • Vivek Prasad
  • Shri Krishna Raj
Article
  • 173 Downloads

Abstract

Papaya ringspot virus (PRSV) is the key pathogen responsible for damage of the papaya plant and significant loss of fruit yield globally. Its coat protein (CP) sequences are highly variable and provide adaptability to the virus in the papaya plant. Due to the variability, the major concern facing of papaya cultivation is the breakdown of CP-transgenic PRSV resistance. In this study, 16 PRSV isolates were collected from different locations in seven districts of Uttar Pradesh, India and the variability of the CP gene of the virus was analysed. Further, it was compared with the isolates from other geographical origins. High diversity in CP sequences of our isolates was observed when compared with other Indian isolates. Phylogenetic analysis also revealed that PRSV isolates were more divergent from isolates of other countries. This study of sequence variation in the CP could be further very useful in virus management and development of broad-spectrum virus-resistant transgenic papaya.

Keywords

Papaya ringspot virus Coat protein Phylogeny 

Notes

Acknowledgements

The authors are thankful to University Grants Commission (UGC), New Delhi, for providing financial assistance as Dr. D.S. Kothari Postdoctoral Fellowship to Rajesh Kumar to carry out this research work.

Compliance with ethical standards

Human and animal studies

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest and informed consent

The authors declare no conflict of interest and all the authors agreed to submit this manuscript in this current version.

Supplementary material

10658_2019_1778_MOESM1_ESM.xlsx (36 kb)
ESM 1 (XLSX 36 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Rajesh Kumar
    • 1
    Email author
  • Ashish Srivastava
    • 2
  • Aparana Srivastava
    • 3
  • Shalini Srivastava
    • 3
  • Vivek Prasad
    • 3
  • Shri Krishna Raj
    • 4
  1. 1.Plant Biotechnology Laboratory, Department of Genetics and Plant Breeding, Rajiv Gandhi South Campus (RGSC)Banaras Hindu UniversityMirzapurIndia
  2. 2.Amity Institute of Virology and ImmunologyAmity UniversityNoidaIndia
  3. 3.Molecular Plant Virology Lab, Department of BotanyUniversity of LucknowLucknowIndia
  4. 4.Molecular Plant VirologyCSIR—National Botanical Research InstituteLucknowIndia

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