Soil fumigation with mustard essential oil to control bacterial wilt in tomato
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In vitro experiments showed that six isolates, belonging to different biovars, were equally susceptible to mustard essential oil (MEO) vapor. The proportion of cell mortality and colony growth inhibition were directly related to the dose of MEO. A 24 h exposure to 1 or 2 μL of AITC per liter of box volume resulted in mortality of 89% and 92% of cells, respectively, and exposure to 10 μL L−1 resulted in 100% cell mortality. The growth of developing colonies was reduced by 89% at 2 or 4 μL L−1 and by 96% at 6 or 10 μL L−1. The leakage of intracellular materials increased with increasing doses of MEO vapor. The decline in soil inoculum density by fumigation was directly related to the MEO dose. The initial inoculum density of approximately 3 × 105 colony-forming units per gram of soil declined by 67, 91 and 96% at fumigation doses of 25, 50 and 75 μL of allyl isothiocyanate (AITC) per liter of soil, respectively. The pathogen was not detected at a fumigation dose of 100 μL L−1. Disease control was also related to the fumigation dose. After 48 days, the bacterial wilt symptoms did not develop on the tomatoes when the soil was fumigated at 100 μL L−1 or higher.
KeywordsMustard essential oil Control of bacterial wilt Plant essential oil Ralstonia solanacearum
The first author would like to express their gratitude to Fundação de Amparo à Pesquisa do Estado de Minas Gerais - FAPEMIG for their M.Sc. fellowship. O. D. Dhingra would like to thank CNPq for his research fellowship.
This study was funded by Fundação de Amparo à Pesquisa do Estado de Minas Gerais - FAPEMIG
Compliance with ethical standards
Conflict of Interest
The authors declare that they have no conflict of interest.
Research involving human participants and or animals
This article does not contain any studies with human participants or animals performed by any of the authors.
Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.
- Abd-Alla, M. A., & Haggag, W. M. (2013). Use of some plant essential oils as post-harvest botanical fungicides in the management of anthracnose disease of mango fruits (Mangi feraindica L.) caused by Colletotrichum gloeosporioides (penz). International Journal of Agriculture and Forestry, 3(1), 1–6.Google Scholar
- Arthy, J. R., Akiew, E. B., Kirkegaard, J. A., & Trevorrow, P. R. (2005). Using Brassica spp. as biofumigants to reduce the population of Ralstonia solanacearum. In C. Allen, P. Prior, & A. C. Hayward (Eds.), Bacterial wilt disease and the Ralstonia solanacearum species complex (pp. 159–165). St. Paul: American Phytopathological Society Press.Google Scholar
- Bandyopadhyay, S., & Khalko, S. (2016). Biofumigation-an eco-friendly approach for managing bacterial wilt and soft rot disease of ginger. Indian Phytopathology, 69(1), 53–56.Google Scholar
- Dhingra, O. D., Jham, G. N., Rodrigues, F. A., Silva, G. J., Jr., & Costa, M. L. N. (2009a). Fumigation with essential oil of mustard retards fungal growth and accumulation of ergosterol and free fatty acid in stored shelled groundnuts. Journal of Stored Products Research, 45(1), 24–31.CrossRefGoogle Scholar
- Elphinstone, J. G. (2005). The current bacterial wilt situation: a global overview. In C. Allen, P. Prior, & A. C. Hayward (Eds.), Bacterial Wilt: the disease and the Ralstonia solanacearum species complex (pp. 9–28). St. Paul: APS Press.Google Scholar
- Kelman, A. (1954). The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology, 44(12), 693–695.Google Scholar
- Kirkegaard, J. A., Sarwar, M., & Matthiessen, J. N. (1998). Assessing the biofumigation potential of crucifers. Acta Horticulturae, (459, 1), 105–112.Google Scholar
- Lage, D. A. C. (2009). Fumigação do solo com óleo essencial de mostarda para o controle da murcha de fusário em tomateiro. Viçosa: Universidade Federal de Viçosa.Google Scholar
- Messiha, N., van Diepeningen, A., Wenneker, M., van Beuningen, A., Janse, J., Coenen, T., Termorshuizen, A., van Bruggen, A., & Blok, W. (2007). Biological soil disinfestation (BSD), a new control method for potato brown rot, caused by Ralstonia solanacearum race 3 biovar 2. European Journal of Plant Pathology, 117(4), 403–415.CrossRefGoogle Scholar
- Olivier, A. R., Uda, Y., Bang, S. W., Honjo, H., Fukami, M., & Fukui, R. (2006). Dried residues of specific cruciferous plants incorporated into soil can suppress the growth of Ralstonia solanacearum, independently of glucosinolate content of the residues. Microbes and Environments, 21(4), 216–226.CrossRefGoogle Scholar
- Ren, Z., Li, Y., Fang, W., Yan, D., Huang, B., Zhu, J., Wang, X., Wang, X., Wang, Q., Guo, M. & Cao, A. (2018). Evaluation of allyl isothiocyanate as a soil fumigant against soil‐borne diseases in commercial tomato (Lycopersicon esculentum Mill.) production in China. Pest Management Science, 74(9), 2146–2155.Google Scholar
- Schaad, N. W., Jones, J. B., & Chun, W. (2001). Laboratory guide for identification of plant pathogenic bacteria (3th ed.). Minnesota: American Phytopathological Society.Google Scholar
- Schurt, D. A. (2006). Potencial do isotiocianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorum. Viçosa: Universidade Federal de Viçosa.Google Scholar
- Sharma, J. P., & Kumar, S. (2004). Effect of crop rotation on population dynamics of Ralstonia solanacearum in tomato wilt sick soil. Indian Phytopathology, 57(1), 80–81.Google Scholar
- van Elsas, J. D., van Overbeek, L. S., Bailey, M. J., Schönfeld, J., & Smalla, K. (2005). Fate of Ralstonia solanacearum biovar 2 as affected by conditions and soil treatments in temperate climate zones. In C. Allen, P. Prior, & A. C. Hayward (Eds.), Bacterial wilt disease and the Ralstonia solanacearum species complex. St. Paul: American Phytopathological Society Press.Google Scholar