Efficacy of vatica oil in controlling Aspergillus parasiticus in maize grain by direct contact and fumigation methods
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The objectives of the study were to test and compare the efficacy of essential oils and their derivatives for control of the aflatoxin-producing fungi Aspergillus parasiticus on contaminated maize grain. Among the five essential oils tested, vatica oil completely inhibited the growth and conidia germination of A. parasiticus TISTR 3276 by both methods. Benzyl acetate was also effective against the pathogen. The minimum inhibitory concentration of vatica oil and benzyl acetate against the fungal growth by direct contact was 10 μL mL−1 while it was 50 μL L−1 for the fumigation assay. Exposure to vatica oil at 10 μL mL−1 for 120 min could completely kill the conidia of the aflatoxin producing fungi while benzyl acetate showed antifungal activity but not rapid killing. SEM results illustrated that the direct contact method completely inhibited the conidia germination while the fumigation assay exhibited ultrastructure alterations of the conidia and abnormal growth of the fungal strain. Fumigation using vatica oil and benzyl acetate at their effective concentrations (10 μL mL−1 and 50 μL L−1, respectively) decreased the contamination of A. parasiticus TISTR 3276 on maize grain. Moreover, both vatica oil and benzyl acetate also protected and cured the contaminated maize grain. Thus, vatica essential oil and benzyl acetate have potential use in the control of aflatoxin producing fungi A. parasiticus.
KeywordsAspergillus parasiticus Vatica oil Benzyl acetate Direct contact Fumigation
This research work was financially supported by the Agricultural Research Development Agency (Public Organization) (PRP5905021490) and Thailand Research Fund (RTA6080010).
Compliance with ethical standards
Conflict of interest
The authors declare having no conflict of interest.
Human and animal studies
This research did not involve human and/or animal participants.
- Bernardos, A., Marina, T., Žácek, P., Pérez-Esteve, E., Martínez-Mañez, R., Lhotka, M., Kourimská, L., Pulkrábeka, J., & Kloucek, P. (2015). Antifungal effect of essential oil components against Aspergillus niger when loaded into silica mesoporous supports. Journal of the Science of Food and Agriculture, 95, 2824–2831.CrossRefGoogle Scholar
- Boukaew, S., Prasertsan, P., & Sattayasamitsathit, S. (2017). Evaluation of antifungal activity of essential oils against aflatoxigenic Aspergillus flavus and their allelopathic activity from fumigation to protect maize seeds during storage. Industrial Crops and Products, 97, 558–566.CrossRefGoogle Scholar
- Cardiet, G., Fuzeau, B., Barreau, C., & Fleurat-Lessard, F. (2012). Contact and fumigant toxicity of some essential oil constituents against a grain insect pest Sitophilus oryzae and two fungi, Aspergillus westerdijkiae and Fusarium graminearum. Journal of Pest Science, 85, 351–358.CrossRefGoogle Scholar
- dos Santos, N. S. T., Aguiar, A. J. A. A., de Oliveira, C. E. V., de Sales, C. V., de Meloe Silva, S., da Silva, R. S., Stamford, T. C. M., & de Souza, E. L. (2012). Efficacy of the application of a coating composed of chitosan and Origanum vulgare L. essential oil to control Rhizopus stolonifer and Aspergillus niger in grapes (Vitis Labrusca L.). Food Microbiology, 32, 345–353.CrossRefGoogle Scholar
- Guo, B. N. (2000). Control of preharvest aflatoxin contamination in corn: Fungus plant-insect interactions and control strategies. Recent Research Developments in Agricultural and Food Chemistry, 4, 165–176.Google Scholar
- Guterman, I., Masci, T., Chen, X., Negre, F., Pichersky, E., Dudareva, N., Weiss, D., & Vainstein, A. (2006). Generation of phenylpropanoid pathway-derived volatiles in transgenic plants: rose alcohol acetyltransferase produces phenylethyl acetate and benzyl acetate in petunia flowers. Plant Molecular Biology, 60, 555–563.CrossRefGoogle Scholar
- Kohiyama, C. Y., Ribeiro, M. M. Y., Mossini, S. A. G., Bando, E., da Silva Bomfim, N., Nerilo, S. B., Rocha, G. H. O., Grespan, R., Mikcha, J. M. G., & Machinski, M., Jr. (2015). Antifungal properties and inhibitory effects upon aflatoxin production of Thymus vulgaris L. by Aspergillus flavus Link. Food Chemistry, 173, 1006–1010.CrossRefGoogle Scholar
- Nerilo, S. B., Rocha, G. H. O., Tomoike, C., Mossini, S. A. G., Grespan, R., Mikcha, J. M. G., & Machinski, M., Jr. (2016). Antifungal properties and inhibitory effects upon aflatoxin production by Zingiber officinale essential oil in Aspergillus flavus. International Journal of Food Science and Technology, 51, 286–292.CrossRefGoogle Scholar
- Passone, M. A., Girardi, N. S., & Etcheverry, M. (2012). Evaluation of the control ability of five essential oils against Aspergillus section Nigri growth and ochratoxinA accumulation in peanut meal extract agar conditioned at different water activities levels. International Journal of Food Microbiology, 159, 198–206.CrossRefGoogle Scholar
- Pérez-Alfonso, C. O., Martínez-Romero, D., Zapata, P. J., Serrano, M., Valero, D., & Castillo, S. (2012). The effects of essential oils carvacrol and thymol on growth of Penicillium digitatum and P. italicum involved in lemon decay. International Journal of Food Microbiology, 158, 101–106.CrossRefGoogle Scholar
- Pinto, E., Gonçalves, M. J., Hrimpeng, K., Pinto, J., Vaz, S., Vale-Silva, L. A., Cavaleiro, C., & Salgueiro, L. (2013). Antifungal activity of the essential oil of Thymus villosus subsp. lusitanicus against Candida, Cryptococcus, Aspergillus and dermatophyte species. Industrial Crops and Products, 51, 93–99.CrossRefGoogle Scholar
- Prakash, B., Singh, P., Mishra, P. K., & Dubey, N. K. (2012b). Safety assessment of Zanthoxylum alatum Roxb. essential oil, its antifungal, antiaflatoxin, antioxidant activity and efficacy as antimicrobial in preservation of Piper nigrum L. fruits. International Journal of Food Microbiology, 153, 183–191.CrossRefGoogle Scholar
- Razzaghi-Abyaneh, M., Shams-Ghahfarokhi, M., Rezaee, M. B., Jaimand, K., Alinezhad, S., Saberi, R., & Yoshinari, T. (2009). Chemical composition and antiaflatoxigenic activity of Carumcarvi L., Thymus vulgaris and Citrus aurantifolia essential oils. Food Control, 20, 1018–1024.CrossRefGoogle Scholar
- Tolouee, M., Alinezhad, S., Saberi, R., Eslamifar, A., Zad, S. J., Jaimand, K., Taeb, J., Rezaee, M. B., Kawachi, M., Shams-Ghahfarokhi, M., & Razzaghi-Abyaneh, M. (2000). Effect of Matricaria chamomilla L. flower essential oil on the growth and ultrastructure of Aspergillus niger van Tieghem. International Journal of Food Microbiology, 139, 127–133.CrossRefGoogle Scholar
- Vilela, G. R., Almeida, G. S., Darce, M. A. B. R., Moraes, M. H. D., Brito, J. O., Silva, M. F. G. F., Silva, S. C., Piedade, S. M. S., Calori-Domingues, M. A., & Gloria, E. M. (2009). Activity of essential oil and its major compound, 1.8-cineole, from Eucalyptus globules Labill, against the storage fungi Aspergillus flavus Link and Aspergillus parasiticus Speare. Journal of Stored Products Research, 45, 1–4.CrossRefGoogle Scholar
- Wenda-Piesik, A. (2011). Volatile organic compound emissions by winter wheat plants (Triticum aestivum L.) under Fusarium spp. infestation and various antibiotic conditions. Polish Journal of Environmental Studies, 20, 1335–1342.Google Scholar