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Efficacy of vatica oil in controlling Aspergillus parasiticus in maize grain by direct contact and fumigation methods

  • Sawai BoukaewEmail author
  • Wanida Petlamul
  • Purawich Phitthayaphinant
  • Poonsuk PrasertsanEmail author
Article
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Abstract

The objectives of the study were to test and compare the efficacy of essential oils and their derivatives for control of the aflatoxin-producing fungi Aspergillus parasiticus on contaminated maize grain. Among the five essential oils tested, vatica oil completely inhibited the growth and conidia germination of A. parasiticus TISTR 3276 by both methods. Benzyl acetate was also effective against the pathogen. The minimum inhibitory concentration of vatica oil and benzyl acetate against the fungal growth by direct contact was 10 μL mL−1 while it was 50 μL L−1 for the fumigation assay. Exposure to vatica oil at 10 μL mL−1 for 120 min could completely kill the conidia of the aflatoxin producing fungi while benzyl acetate showed antifungal activity but not rapid killing. SEM results illustrated that the direct contact method completely inhibited the conidia germination while the fumigation assay exhibited ultrastructure alterations of the conidia and abnormal growth of the fungal strain. Fumigation using vatica oil and benzyl acetate at their effective concentrations (10 μL mL−1 and 50 μL L−1, respectively) decreased the contamination of A. parasiticus TISTR 3276 on maize grain. Moreover, both vatica oil and benzyl acetate also protected and cured the contaminated maize grain. Thus, vatica essential oil and benzyl acetate have potential use in the control of aflatoxin producing fungi A. parasiticus.

Keywords

Aspergillus parasiticus Vatica oil Benzyl acetate Direct contact Fumigation 

Notes

Acknowledgements

This research work was financially supported by the Agricultural Research Development Agency (Public Organization) (PRP5905021490) and Thailand Research Fund (RTA6080010).

Compliance with ethical standards

Conflict of interest

The authors declare having no conflict of interest.

Human and animal studies

This research did not involve human and/or animal participants.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  1. 1.College of Innovation and ManagementSongkhla Rajabhat UniversitySongkhlaThailand
  2. 2.Faculty of Technology and Community DevelopmentThaksin UniversityPhatthalungThailand
  3. 3.Research and Development OfficePrince of Songkla UniversitySongkhlaThailand

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