Pollen and seed transmission of Columnea latent viroid in eggplants

  • Samabhorn Bhuvitarkorn
  • Kanungnit ReanwarakornEmail author


Viroid seed transmission data are important for plant production because such information can be very helpful in preventing the movement of viroids worldwide via infected seed. In this study, the reverse transcription-polymerase chain reaction (RT-PCR) technique was used with columnea latent viroid (CLVd) specific primers to investigate viroid seed transmission from three infected eggplant (Solanum melongena L.) genotypes through cross-pollination to seeds and seedlings. Pollen was harvested from CLVd-infected eggplant plants and used to cross-pollinate healthy plants (recipient parents) to produce seeds for the CLVd seed transmission test. CLVd that was localized on the seed coat and in the seedlings was determined using a ‘top-of-paper’ germination technique. The RT-PCR results showed positive results, indicating that CLVd was located in both the seed coat and the seedling. The RT-PCR test performed on 8 week-old seedlings of ‘Farmers Long’, ‘Jamaica’ and ‘RPG’ showed that the seed transmission rates were 82%, 6.4% and 2.3%, respectively. Based on this work, CLVd seed transmission was confirmed in eggplant. Furthermore, these results indicated that CLVd-infected pollen carries the risk of transmitting the viroid, during cross-pollination, from a donor eggplant to its progeny.


Viroid Columnea latent viroid Eggplant Pollen transmission Seed transmission 



This work was partially supported by: the Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University Under the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, Ministry of Education, Thailand; the Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Office of Higher Education Commission, Ministry of Education (AG-BIO/PERDO-CHE); the Agricultural Research Development Agency (Public Organization); the National Research Council of Thailand (NRCT); the Kasetsart University Research and Development Institute (KURDI); and the Department of Plant Pathology, Kasetsart University, Kamphaeng Saen campus, Thailand.

Compliance with ethical standards

Disclosure of potential conflicts of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This chapter does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Samabhorn Bhuvitarkorn
    • 1
    • 2
    • 3
    • 4
  • Kanungnit Reanwarakorn
    • 1
    • 2
    • 3
    • 4
    Email author
  1. 1.Department of Plant Pathology, Faculty of Agriculture at Kamphaeng SaenKasetsart UniversityNakhon PathomThailand
  2. 2.Center for Advanced Studies for Agriculture and Food, KU Institute for Advanced StudiesKasetsart UniversityBangkokThailand
  3. 3.Center for Agricultural BiotechnologyKasetsart UniversityNakhon PathomThailand
  4. 4.Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE)BangkokThailand

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