Phenotypical and genotypical characterization of Pyrenophora tritici-repentis races in Brazil

  • Victória Vieira BertagnolliEmail author
  • Jéssica Rosset Ferreira
  • Zhaohui Liu
  • André Cunha Rosa
  • Carolina Cardoso Deuner


Tan spot, caused by Pyrenophora tritici-repentis, is one of the major wheat diseases in southern Brazil as well as in the wheat production areas of several other countries. The fungal isolates have been classified into eight races according to the production of three fungal necrotrophic effectors. The knowledge about the pathogen population structure is essential for disease control strategies, but such information is lacking in Brazil. The aim of this study was to determine the P. tritici-repentis race structure of Brazilian isolates using, for the first time, biological and molecular techniques. P. tritici-repentis isolates were characterized for their virulence on a set of wheat genotypes, and for the presence of ToxA and ToxB gene. The results from the PCR amplification with the gene specific primers showed that all Brazilian P. tritici-repentis isolates carried ToxA, but no ToxB is present. Race typing on the set of wheat genotypes indicate 65% of the isolates were race 2 while 35% were race 1. This is the first study on the P. tritici-repentis race structure in Brazil and the information will be useful to the local wheat breeders who aim to improve tan spot resistance in wheat cultivars.


Differential set Isolates Tan spot Toxins Wheat 



We are thankful to Passo Fundo University and North Dakota State University for the facility, Capes (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for financial support, Biotrigo Genética Ltda. for the networking support, Dr. Flávio Martins Santana and his team from Embrapa Trigo for the concession of some isolates, and MSc. Augusto Bittencourt Vieira for helping with the English review.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animals rights

No human and/or animal participants were involved in this research.

Informed consent

All authors consent to this submission.

Supplementary material

10658_2019_1720_Fig6_ESM.png (696 kb)
Supplementary Figure 1

BLAST search. A) Results of blast search considering the sequence of S30 isolate (accession MK490983). B). Sequence of Pyrenophora chaetomioides presenting 100% of identities with S30 isolate sequence. (PNG 695 kb)

10658_2019_1720_MOESM1_ESM.tif (1.1 mb)
High Resolution (TIF 1120 kb)
10658_2019_1720_Fig7_ESM.png (642 kb)
Supplementary Figure 2

BLAST search. A) Results of blast search considering the sequence of S31 isolate (accession MK490984). B). Sequence of Drechslera avenae presenting 99% of identities with S31 isolate sequence. (PNG 642 kb)

10658_2019_1720_MOESM2_ESM.tif (1 mb)
High Resolution (TIF 1048 kb)


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Victória Vieira Bertagnolli
    • 1
    Email author
  • Jéssica Rosset Ferreira
    • 1
  • Zhaohui Liu
    • 2
  • André Cunha Rosa
    • 3
  • Carolina Cardoso Deuner
    • 1
  1. 1.Faculdade de Agronomia e Medicina VeterináriaUniversidade de Passo FundoPasso FundoBrazil
  2. 2.Department of Plant PathologyNorth Dakota State UniversityNorth DakotaUSA
  3. 3.Biotrigo Genética LtdaPasso FundoBrazil

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