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Distribution of chrysanthemum chlorotic mottle viroid in shoot meristem and flower buds of chrysanthemum

  • Mami Ebata
  • Yosuke Matsushita
  • Masayuki Morimoto
  • Tomofumi MochizukiEmail author
Article
  • 79 Downloads

Abstract

Detailed information on viroid invasion of the shoot apical meristem is important for the efficient production of viroid-free plants by meristem tip culture. Additionally, knowledge on viroid distribution in plant reproductive tissues, especially the embryo, is crucial to assess seed transmission of viroids. In this study, we analyzed histochemically the distribution of chrysanthemum chlorotic mottle viroid (CChMVd) in the shoot meristems and flower buds of CChMVd-infected chrysanthemum plants (cv. ‘Okayama-heiwa’). In situ hybridization revealed that CChMVd invaded the shoot apical meristems and leaf primordia in the shoot meristem at a high frequency (ca. 70%). In flower buds, CChMVd was found in the bract, receptacle vascular, petals, ovules, and immature stamens and pistils. Furthermore, we showed that seed transmission of CChMVd occurred at a high frequency when CChMVd-infected plants were used as female parents (♀). Overall, our findings suggest that CChMVd infection of ovules in flower buds is involved in seed transmission of the viroid.

Keywords

Chrysanthemum Flower bud In situ hybridization: seed transmission Shoot apical meristem Viroid 

Notes

Compliance with ethical standards

Conflict of interest

All the authors declare that they have no conflict of interest.

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  1. 1.Graduate School of Life and Environmental SciencesOsaka Prefecture UniversityOsakaJapan
  2. 2.Institute of Vegetable and Floriculture ScienceNational Agriculture and Food Research OrganizationIbarakiJapan
  3. 3.Japan Agribio Company LimitedShizuokaJapan

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