Characterizing Beltrania pseudorhombica the causal agent of pistachio leaf and fruit spot in Arizona

  • P. S. F. LichtembergEmail author
  • J. Moral
  • J. Sherman
  • M. T. Nouri
  • J. Lake
  • D. G. Felts
  • T. J. MichailidesEmail author


In 2016 and 2017, we have constantly recovered a fungus with typical biconical (diamond shaped) conidia from pistachio fruit (cv. Kerman) received from Cochise County, Arizona, United States. Morphological and molecular characterization identified the isolates as Beltrania pseudorhombica, a member of the Beltraniaceae family that is an uncommon plant pathogen. In in-vitro tests, B. pseudorhombica showed temperatures of 26.9 °C and 31.1 °C as the optimal for the mycelial growth and conidial germination, respectively. Pathogenicity tests demonstrated the isolate capacity to cause lesion in leaflets (Kerman > Lost Hills > Golden Hills > Kalehghouchi) and fruits (Lost Hills > Kalehghouchi > Golden Hills > Kerman). The preventive fungicide application with sole formulation of demethylation inhibitor and mixed formulation of succinate dehydrogenase inhibitors and quinone outside inhibitors offered the greatest lesion control, when compared with the sole formulation of quinone outside inhibitors. This short communication provides important epidemiological information that can be useful to avoid the dissemination of new pathogens in new pistachio production areas in the United States.


Beltraniaceae Fungicide efficacy Pathogenicity Pistacia vera 



The authors thank the California Pistachio Research Board for financial support, the AZ pistachio growers for allowing us to collect field samples, and to Dr. Pedro Crous for useful discussions.

Supplementary material

10658_2019_1675_MOESM1_ESM.docx (15 kb)
ESM 1 (DOCX 14 kb)


  1. ACP (2018). 2017 Pistachio bearing acreage, production and yield per acre by district and county. (pp. 4). Administrative Committee for Pistachios. Accessed Aug 2018.
  2. Blank, S. (2016). The economic outlook for California pistachio. In L. Ferguson, & D. Haviland (Eds.),Pistachio production manual (pp. 3:10, Vol. Publication 3545). Oakland, CA: University of California Agriculture and Natural Resources.Google Scholar
  3. Box, G. E., & Cox, D. R. (1964). An analysis of transformations. Journal of Royal Statistical Society, Series B, 26(2), 211–252.Google Scholar
  4. Chen, S., Morgan, D., & Michailides, T. (2013). First report of Phoma fungicola associated with stem canker and fruit blight of pistachio. Journal of Plant Pathology, 95(2), 451.Google Scholar
  5. Crous, P., Shivas, R., Quaedvlieg, W., Van der Bank, M., Zhang, Y., Summerell, B., et al. (2014). Fungal planet description sheets: 214–280. Persoonia: Molecular Phylogeny and Evolution of Fungi, 32, 184–306.CrossRefGoogle Scholar
  6. Crous, P., Wingfield, M. J., Le Roux, J. J., Richardson, D. M., Strasberg, D., Shivas, R. G., et al. (2015). Fungal planet description sheets: 371-399. Persoonia: Molecular Phylogeny and Evolution of Fungi, 35, 264–327.CrossRefGoogle Scholar
  7. Lichtemberg, P., Moral, J., Morgan, D., Felts, D., Sanders, R., & Michailides, T. (2017). First report of anthracnose caused by Colletotrichum fioriniae and C. karstii in California pistachio orchards. Plant Disease, 101(7), 1320.CrossRefGoogle Scholar
  8. Lin, C.-G., Hyde, K. D., Lumyong, S., & Mckenzie, E. H. (2017). Beltrania-like taxa from Thailand. Cryptogamie, Mycologie, 38(3), 301–319.CrossRefGoogle Scholar
  9. Mahoney, N. E., Gee, W. S., Higbee, B. S., & Beck, J. J. (2014). Ex situ volatile survey of ground almond and pistachio hulls for emission of spiroketals: Analysis of hull fatty acid composition, water content, and water activity. Phytochemistry Letters, 7, 225–230.CrossRefGoogle Scholar
  10. Michailides, T., Morgan, D., & Doster, M. (2016). Foliar, fruit, and branch diseases. In L. Ferguson, & D. Haviland (Eds.), Pistachio production manual (pp. 265:292, Vol. Publication 3545). Oakland, CA: University of California Agriculture and Natural Resources.Google Scholar
  11. Moral, J., Muñoz-Díez, C., González, N., Trapero, A., & Michailides, T. (2010). Characterization and pathogenicity of Botryosphaeriaceae species collected from olive and other hosts in Spain and California. Phytopathology, 100(12), 1340–1351.CrossRefGoogle Scholar
  12. Moral, J., Lichtemberg, P., Papagelis, A., Sherman, J., & Michailides, T. (2018). Didymella glomerata causing leaf blight on pistachio. European Journal of Plant Pathology, 151(4), 1095–1099.CrossRefGoogle Scholar
  13. Pryor, B. M., & Michailides, T. J. (2002). Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology, 92(4), 406–416.CrossRefGoogle Scholar
  14. Rajeshkumar, K. C., Crous, P. W., Groenewald, J. Z., & Seifert, K. A. (2016). Resolving the phylogenetic placement of Porobeltraniella and allied genera in the Beltraniaceae. Mycological Progress, 15(10–11), 1119–1136.CrossRefGoogle Scholar
  15. Shi, Z., Xiang, M., Zhang, Y., & Huang, J. (2012). First report of leaf spot on Tibouchina semidecandra caused by Beltrania rhombica in China. Plant Disease, 96(9), 1380–1380.CrossRefGoogle Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  1. 1.Department of Plant PathologyUniversity of California - Davis/Kearney Agricultural Research and Extension CenterParlierUSA
  2. 2.Department of Plant PathologyUniversity of CórdobaCórdobaSpain
  3. 3.Department of Plant Science and MicrobiologyUniversity of ArizonaWillcoxUSA

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