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European Journal of Plant Pathology

, Volume 153, Issue 3, pp 869–878 | Cite as

Anastomosis group and pathogenicity of Rhizoctonia spp. associated with seedling damping-off of sugar beet in China

  • Can Zhao
  • Yuting Li
  • Siying Wu
  • Pinpin Wang
  • Chenggui Han
  • Xuehong WuEmail author
Article

Abstract

Between 2010 and 2015, 204 Rhizoctonia isolates were obtained from diseased sugar beet seedlings which collected from the sugar beet growing regions of China. On the basis of the morphological characteristics and sequence analysis of the internal transcribed spacers of the ribosomal DNA (rDNA ITS), 166 were identified as multinucleate Rhizoctonia (MNR), and 38 were binucleate Rhizoctonia (BNR), belonging to 15 species, anastomosis groups (AGs) or subgroups. The most prevalent was Rhizoctonia solani AG-4, with 49 isolates attributed to the subgroup HGIII, 47 to HGII, and 45 to HGI, followed by AG-K (31), AG-2-2IIIB (7), AG-A (6), AG-1-1C (4), AG-1-1B (3), AG-2-1 (3), AG-5 (3), AG-11 (2), AG-3 TB (1), Waitea circinata var. circinata (1), W. circinata var. zeae (1), and AG-C (1). Seventy-four representative isolates of all species, AGs or subgroups from different geographic origins were able to cause brown, dry lesions on roots of sugar beet seedlings or even seedling death with the disease incidence and the disease index ranging from 13.3 to 100% and from 1.9 to 97.1, respectively. The AG-4 isolates were the most aggressive on sugar beet seedlings. Moreover, the aggressiveness of the R. solani isolates was higher than that of the other isolates. To the best of our knowledge, this is the first detailed description of the AG composition of Rhizoctonia causing seedling damping-off on sugar beet in China.

Keywords

Sugar beet Rhizoctonia Anastomosis group Pathogenicity Internal transcribed spacer DNA sequencing 

Notes

Acknowledgements

This work was supported by the earmarked fund for China Agriculture Research System (CARS-170304). Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal studies

This article does not contain any studies with human or animal subjects.

Supplementary material

10658_2018_1603_MOESM1_ESM.doc (270 kb)
ESM 1 (DOC 269 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Can Zhao
    • 1
  • Yuting Li
    • 1
  • Siying Wu
    • 1
  • Pinpin Wang
    • 1
  • Chenggui Han
    • 1
  • Xuehong Wu
    • 1
    Email author
  1. 1.Department of Plant PathologyChina Agricultural UniversityBeijingPeople’s Republic of China

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