In vitro reproduction of Pratylenchus neglectus on carrot discs and investigation of its interaction with Meloidogyne hispanica on potato
- 238 Downloads
Root-lesion nematodes, Pratylenchus spp., and root-knot nematodes, Meloidogyne spp., can occur concomitantly in potato fields and the interactions between the two groups of plant parasitic nematodes (PPN) may represent a threat to the potato crop. The in vitro reproductive fitness of 3 Portuguese isolates of P. neglectus isolated from potato fields was assessed using monoxenic carrot disc cultures inoculated with 10 females and maintained at 25 °C. The three isolates were able to reproduce at 56 days after inoculation (DAI) and the final population densities were significantly increased at 84 DAI. The P. neglectus isolate with the best reproductive ability was further selected and the concomitant effects of P. neglectus and M. hispanica were investigated in a pot trial using potato (Solanum tuberosum L.) cv. Agria as host. Single potato sprouts were inoculated with 250, 500, 750 and 1000 of either P. neglectus mixed stages/M. hispanica second-stage juveniles, or a combination of both nematodes. At 60 DAI, nematodes were extracted from roots and soil, the final population densities determined, for both nematode species, and the reproductive factor calculated. The experiment was run twice. The results suggest an additive effect of M. hispanica on P. neglectus reproduction, although future tests are still needed to confirm the possibility of an increase of P. neglectus population densities for the next crop, when both nematodes are inoculated concomitantly. The information obtained reinforces the need for a careful monitoring of these two PPN species in potato crops.
KeywordsNematode reproduction Root-knot nematodes Root-lesion nematodes Solanum tuberosum
This research was supported by CFE, Instituto do Ambiente, Tecnologia e Vida, CIEPQPF and FEDER funds through the Programa Operacional Factores de Competitividade (COMPETE, Portugal) and by FCT (Fundação para a Ciência e a Tecnologia, Portugal), under contracts UID/BIA/04004/2013, PEst-C/EQB/UI0102/2013, FCOMP-01-0124-FEDER-027960 (Ref. PTDC/AGRPRO/2510/2012), POCI-01-0145-FEDER-029392 and Project ReNATURE – Valorization of the Natural Endogenous Resources of the Centro Region (Centro 2020, Centro-01-0145-FEDER-000007). Carla Maleita (SFRH/BPD/85736/2012) and Ivânia Esteves (SFRH/BPD/68856/2010) acknowledge FCT for granted fellowships, financed by Ministério da Educação e Ciência national funding and by the European Social Funds through Programa Operacional Capital Humano.
Compliance with ethical standards
Conflict of interest
The authors certify that do not have any actual or potential conflict of interest, the study described is original, has not been published previously, and is not under consideration for publication elsewhere. All authors have reviewed the manuscript and approved the final version of manuscript before submission.
Human participants and/or animals
No specific permits were required for the described studies.
The authors certify that the work carried out in this research followed the principles of ethical and professional conduct.
- Al-Rehiayani, S., Hafez, S. L., Thornton, M., & Sundararaj, P. (1999). Effects of Pratylenchus neglectus, Bacillus megaterium, and oil radish or rapeseed green manure on reproductive potential of Meloidogyne chitwoodi on potato. Nematropica, 29, 37–49.Google Scholar
- Conceição, I. L. P. M. da, Cunha, M. J. M. da, Feio, G., et al. (2009). Root-knot nematodes, Meloidogyne spp., on potato in Portugal. Nematology, 11, 311–313.Google Scholar
- Gonzaga, V., & Santos, J. M. (2010). Estudo comparativo in vitro de seis espécies de Pratylenchus em cilindros de cenoura. Nematologia Brasileira, 34, 228–230.Google Scholar
- Hafez, S. L., Al-Rehiayani, S., Thornton, M., & Sundararaj, P. (1999). Differentiation of two geographically isolated populations of Pratylenchus neglectus based on their parasitism of potato and interaction with Verticillium dahliae. Nematropica, 29, 25–35.Google Scholar
- Hussey, R. S., & Barker, K. R. (1973). A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter, 57, 1025–1028.Google Scholar
- Kagoda, F., Coyne, D., Mbiru, E., Derera, J., & Tongoona, P. (2010). Monoxenic culture of Pratylenchus zeae on carrot discs. Nematologia Mediterranea, 38, 107–108.Google Scholar
- Karakas, M. (2015). Reproductive potential of the root-lesion nematode Pratylenchus penetrans (Cobb, 1917) Sher and Allen, 1953 (Nematoda:Pratylenchidae) in Monoxenic cultures. European International Journal of Science and Technology, 4, 105–110.Google Scholar
- Nyczepir, A. P. (2009). Dynamics of concomitant populations of Pratylenchus vulnus and Meloidogyne incognita on peach. Nematropica, 39, 273–279.Google Scholar
- O'Bannon, J. H., & Taylor, A. L. (1968). Migratory endoparasitic nematodes reared on carrot discs. Phytopathology, 58, 385.Google Scholar
- Pang, W., Hafez, S. L., & Sundararaj, P. (2009). Concomitant interaction of Pratylenchus penetrans and Meloidogyne hapla on onion. Nematropica, 39, 297–303.Google Scholar
- Taylor, A. L., & Sasser, J. N. (1978). Biology, identification and control of root-knot nematodes (Meloidogyne spp.). A cooperative publication of the Department of Plant Pathology, North Carolina State University and the United States Agency for International Development, Raleigh: North Carolina State University Graphics.Google Scholar
- Van Der Sommen, A. T. C., Van Bruggen, A. S. & Den Nijs, L. J. M. F. (2009). Survey of poorly growing patches in potato fields by helicopter. In International Congress of Tropical Nematology (40th ONTA and 28th SBN meetings), Maceió, Brazil.Google Scholar
- Vovlas, N., Troccoli, A., Minuto, A., Bruzzone, C., & Castillo, P. (2010). Parasitism of the root-knot nematode Meloidogyne hapla on peony in northern Italy. Nematologia Mediterranea, 38, 219–221.Google Scholar