European Journal of Plant Pathology

, Volume 153, Issue 3, pp 957–963 | Cite as

In vitro reproduction of Pratylenchus neglectus on carrot discs and investigation of its interaction with Meloidogyne hispanica on potato

  • Ivânia EstevesEmail author
  • Carla MaleitaEmail author
  • Tânia Pato
  • Isabel Abrantes
  • Maria José Cunha


Root-lesion nematodes, Pratylenchus spp., and root-knot nematodes, Meloidogyne spp., can occur concomitantly in potato fields and the interactions between the two groups of plant parasitic nematodes (PPN) may represent a threat to the potato crop. The in vitro reproductive fitness of 3 Portuguese isolates of P. neglectus isolated from potato fields was assessed using monoxenic carrot disc cultures inoculated with 10 females and maintained at 25 °C. The three isolates were able to reproduce at 56 days after inoculation (DAI) and the final population densities were significantly increased at 84 DAI. The P. neglectus isolate with the best reproductive ability was further selected and the concomitant effects of P. neglectus and M. hispanica were investigated in a pot trial using potato (Solanum tuberosum L.) cv. Agria as host. Single potato sprouts were inoculated with 250, 500, 750 and 1000 of either P. neglectus mixed stages/M. hispanica second-stage juveniles, or a combination of both nematodes. At 60 DAI, nematodes were extracted from roots and soil, the final population densities determined, for both nematode species, and the reproductive factor calculated. The experiment was run twice. The results suggest an additive effect of M. hispanica on P. neglectus reproduction, although future tests are still needed to confirm the possibility of an increase of P. neglectus population densities for the next crop, when both nematodes are inoculated concomitantly. The information obtained reinforces the need for a careful monitoring of these two PPN species in potato crops.


Nematode reproduction Root-knot nematodes Root-lesion nematodes Solanum tuberosum 



This research was supported by CFE, Instituto do Ambiente, Tecnologia e Vida, CIEPQPF and FEDER funds through the Programa Operacional Factores de Competitividade (COMPETE, Portugal) and by FCT (Fundação para a Ciência e a Tecnologia, Portugal), under contracts UID/BIA/04004/2013, PEst-C/EQB/UI0102/2013, FCOMP-01-0124-FEDER-027960 (Ref. PTDC/AGRPRO/2510/2012), POCI-01-0145-FEDER-029392 and Project ReNATURE – Valorization of the Natural Endogenous Resources of the Centro Region (Centro 2020, Centro-01-0145-FEDER-000007). Carla Maleita (SFRH/BPD/85736/2012) and Ivânia Esteves (SFRH/BPD/68856/2010) acknowledge FCT for granted fellowships, financed by Ministério da Educação e Ciência national funding and by the European Social Funds through Programa Operacional Capital Humano.

Compliance with ethical standards

Conflict of interest

The authors certify that do not have any actual or potential conflict of interest, the study described is original, has not been published previously, and is not under consideration for publication elsewhere. All authors have reviewed the manuscript and approved the final version of manuscript before submission.

Human participants and/or animals

No specific permits were required for the described studies.

Informed consent

The authors certify that the work carried out in this research followed the principles of ethical and professional conduct.


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  1. 1.CFE – Centre for Functional Ecology - Science for People & the Planet, Department of Life SciencesUniversity of CoimbraCoimbraPortugal
  2. 2.CIEPQPF – Chemical Process Engineering and Forest Products Research Centre, Chemical Engineering DepartmentUniversity of CoimbraCoimbraPortugal
  3. 3.CERNAS – Centro de Estudos em Recursos Naturais, Ambiente e Sociedade, Department of Agronomic Sciences, High School of AgricultureInstituto Politécnico de CoimbraCoimbraPortugal

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