European Journal of Plant Pathology

, Volume 153, Issue 1, pp 167–181 | Cite as

Effect of natural and synthetic Brassinosteroids on strawberry immune response against Colletotrichum acutatum

  • R. N. Furio
  • P. L. Albornoz
  • Y. Coll
  • G. M. Martínez Zamora
  • S. M. Salazar
  • G. G. Martos
  • J. C. Díaz RicciEmail author


Brassinosteroids (BRs) are steroidal essential compounds for plant growth and development. It was shown that the exogenous applications of BRs induce protection against different pathogens and can give plants tolerance/resistance to different abiotic stresses. The aim of this work was to evaluate the protective effect against the fungal pathogen Colletotrichum acutatum, the causal agent of anthracnose disease, on strawberry plants treated with 24-epibrasinolide (EP24) and a formulation based on a brassinosteroid spirostanic analogue DI-31 (BB16). Treatment with both compounds induced a defense response in strawberry plants of the cv. Pájaro against avirulent isolate (M11) of C. acutatum, being more effective at the lower concentration of both steroids (0.1 mg l−1), although the analogue BB16 showed a stronger effect than EP24. The evaluation of biochemical defense markers showed that strawberry plants treated with EP24 and BB16 increased the production of H2O2, O2.-, NO, calcium oxalate crystals and higher callose and lignin deposition as compared to the control plants. However, stomatal closure was only observed in plants treated with BB16. These results suggests that BB16 and EP24 can be used for the activation of innate immunity in strawberry plants, as a new strategy for crop health protection management, alternative to agrochemicals.


Brassinosteroids Defense Strawberry 



Analysis of variance








4-amino-5-methylamino-2´,7´-difluorofluorescein diacetate


Disease Severity Ratings








2',7'-dichlorodihydrofluorescein diacetate


Induced resistance


Jasmonic acid


2-(N-morpholino) ethanesulfonic acid


Nitro blue tetrazolium


Potato dextrose agar


Reactive oxygen species


Salicylic Acid



This paper was partially supported with grants of the Universidad Nacional de Tucumán (CIUNT 26/D544), and Agencia Nacional de Promoción Científica y Tecnológica (PICT 2013-2075). Authors are grateful to Strawberry Active Germplasm Bank (BGA) from Universidad Nacional de Tucumán (UNT) and Ing. Cecilia Lemme for providing strawberry plants. RNF and GGM are CONICET fellowship, and MGMZ and JCDR is member of CONICET.

Compliance with ethical standards

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The manuscript has not been published and is not under consideration for publication elsewhere. All authors have approved the manuscript and agree with submission to European Journal of Plant Pathology. The research was conducted in the absence of any commercial relationships that could be considered as a potential conflict of interest.


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • R. N. Furio
    • 1
  • P. L. Albornoz
    • 2
  • Y. Coll
    • 3
  • G. M. Martínez Zamora
    • 1
  • S. M. Salazar
    • 4
    • 5
  • G. G. Martos
    • 1
  • J. C. Díaz Ricci
    • 1
    Email author
  1. 1.INSIBIO, CONICET-UNTChacabucoArgentina
  2. 2.Facultad de Ciencias Naturales (UNT), y Fundación Miguel Lillo (FML)San Miguel de TucumánArgentina
  3. 3.Universidad La HabanaHavanaCuba
  4. 4.Instituto Nacional de Tecnología Agropecuaria, EEAFFamailláArgentina
  5. 5.Facultad de Agronomía y Zootecnia, UNTSan Miguel de TucumánArgentina

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