Molecular characterization and pathogenicity of Alternaria species on wheat and date palms in Oman
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This study was conducted to investigate the Alternaria species associated with leaf spot of date palm and wheat in Oman. Out of 98 date palm leaf samples and 146 wheat leaf samples, Alternaria was isolated from 27 and 23% of the samples developing leaf spot symptoms, respectively. Identification of Alternaria isolates using sequences of the internal transcribed spacer region of the ribosomal RNA (ITS rRNA), glyceraldehyde-3-phosphate dehydrogenase (GPDH), translation elongation factor (TEF) and RNA polymerase II subunit (RPB2) genes, showed that the isolates belong to seven Alternaria species or species complexes. A. burnsii - A. tomato and A. arborescens species complexes (58 and 4%, respectively) and A. alternata (38%) were the species recovered from the symptomatic date palm leaves. A. alternata (67%), A. burnsii - A. tomato species complex (15%), A. jacinthicola (3%), A. ventricosa (3%), A. slovaca (6%) and Alternaria caespitosa (6%) were isolated from wheat. Pathogenicity test showed that tested isolates of A. alternata (DPM19, WDK12), A. burnsii - A. tomato species complex (DPM31), A. jacinthicola (WBR4) and A. slovaca (WDK9, WDK7) were pathogenic on date palm, while A. alternata (DPM19, WDK12), A. burnsii - A. tomato species complex (DPM31, WDK11) and A. slovaca (WDK9, WDK7) were pathogenic on wheat. This is the first report of date palm and wheat as new hosts for A. burnsii - A. tomato species complex and the first reports of A. burnsii - A. tomato species complex, A. caespitosa A. slovaca, and A. ventricosa in Oman. The study shows that several species of Alternaria are associated with leaf spot in date palm and wheat in Oman, with some isolates having the ability to cause infection in both hosts.
KeywordsDate palms Pathogenicity Phylogeny Section Alternaria Section Infectoriae Wheat
Authors would like to acknowledge financial support to the study from Sultan Qaboos University and Oman Animal and Plant Genetic Resources Center through the projects IG/AGR/CROP/13/01 and EG/AGR/CROP/16/01. Thanks are due to farmers and extension officers for their help in the collection of samples.
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Conflict of interest
This is to confirm that this manuscript was not submitted for publication to any other journal. It is only submitted to this journal, EJPP. Authors have no conflict of interest.
- Abed, R. M. M., Al-Sadi, A. M., Al-Shehi, M., Al-Hinai, S., & Robinson, M. D. (2013). Diversity of free-living and lichenized fungal communities in biological soil crusts of the Sultanate of Oman and their role in improving soil properties. Soil Biology and Biochemistry, 57, 695–705. https://doi.org/10.1016/j.soilbio.2012.07.023.CrossRefGoogle Scholar
- Al-Sadi, A. M., Al-Masoodi, R. S., Al-Ismaili, M., & Al-Mahmooli, I. H. (2015). Population structure and development of resistance to hymexazol among Fusarium solani populations from date palm, citrus and cucumber. Journal of Phytopathology, 163(11-12), 947–955. https://doi.org/10.1111/jph.12397.CrossRefGoogle Scholar
- Duan, S., Ma, X., Chen, W., Wan, W., He, Y., Ma, Y., et al. (2016). Transcriptomic profile of tobacco in response to Alternaria longipes and Alternaria alternata infections. Scientific Reports, 6. https://doi.org/10.1038/srep25635.
- Esmaeili Taheri, A., Chatterton, S., Foroud, N. A., Gossen, B. D., & McLaren, D. L. (2017). Identification and community dynamics of fungi associated with root, crown, and foot rot of field pea in western Canada. European Journal of Plant Pathology, 147(3), 489–500. https://doi.org/10.1007/s10658-016-1017-4.CrossRefGoogle Scholar
- FAO (2015). FAOSTAT. http://faostat3.fao.org/browse/rankings/countries_by_commodity/E5 October 2016.
- Gannibal, P. B., Orina, A. S., Mironenko, N. V., & Levitin, M. M. (2014). Differentiation of the closely related species, Alternaria solani and A. tomatophila, by molecular and morphological features and aggressiveness. European Journal of Plant Pathology, 139(3), 609–623. https://doi.org/10.1007/s10658-014-0417-6.CrossRefGoogle Scholar
- Gur, L., Reuveni, M., & Cohen, Y. (2017). Occurrence and etiology of Alternaria leaf blotch and fruit spot of apple caused by Alternaria alternata f. sp. mali on cv. Pink lady in Israel. European Journal of Plant Pathology, 147(3), 695–708. https://doi.org/10.1007/s10658-016-1037-0.CrossRefGoogle Scholar
- Kahl, S. M., Ulrich, A., Kirichenko, A. A., & Müller, M. E. H. (2015). Phenotypic and phylogenetic segregation of Alternaria infectoria from small-spored Alternaria species isolated from wheat in Germany and Russia. Journal of Applied Microbiology, 119(6), 1637–1650. https://doi.org/10.1111/jam.12951.CrossRefPubMedGoogle Scholar
- Lawrence, D. P., Rotondo, F., & Gannibal, P. B. (2016). Biodiversity and taxonomy of the pleomorphic genus Alternaria. Mycological Progress, 15(1). https://doi.org/10.1007/s11557-015-1144-x.
- Moghal, S. M., Shivanathan, P., Mani, A., Al-Zidjali, A. D., Al-Zidjali, T. S., & Al-Raeesy, Y. M. (1993). Status of Pests and Diseases in Oman: Series 1: Plant Diseases in the Batinah. Muscat: Ministry of Agriculture and Fisheries.Google Scholar
- Moslemi, A., Ades, P. K., Groom, T., Nicolas, M. E., & Taylor, P. W. J. (2017). Alternaria infectoria and Stemphylium herbarum, two new pathogens of pyrethrum (Tanacetum cinerariifolium) in Australia. Australasian Plant Pathology, 46(1), 91–101. https://doi.org/10.1007/s13313-016-0463-y.CrossRefGoogle Scholar
- Pavón, M. A., González, I., Rojas, M., Pegels, N., Martín, R., & García, T. (2011). PCR detection of alternaria spp. in processed foods, based on the internal transcribed spacer genetic marker. Journal of Food Protection, 74(2), 240–247. https://doi.org/10.4315/0362-028x.jfp-10-110.CrossRefPubMedGoogle Scholar
- Shakir, A. S., Mirza, J. H., Sahi, S. T., & Ansar, M. (1995). First report of Alternaria burnsii: The causal organism of cumin blight in Pakistan. Pakistan Journal of Phytopathology, 7(2), 219.Google Scholar
- Shinha, K. K., & Bhatnagar, D. (1998). Mycotoxins in Agriculture and Food Safety: Marcel Decker Inc. New York.Google Scholar
- Villalobos, M. D. C., Serradilla, M. J., Martín, A., Hernández-León, A., Ruíz-Moyano, S., & Córdoba, M. D. G. (2017). Characterization of microbial population of breba and main crops (Ficus carica) during cold storage: Influence of passive modified atmospheres (MAP) and antimicrobial extract application. Food Microbiology, 63, 35–46. https://doi.org/10.1016/j.fm.2016.10.035.CrossRefPubMedGoogle Scholar
- Woudenberg, J. H. C., Seidl, M. F., Groenewald, J. Z., de Vries, M., Stielow, J. B., Thomma, B. P. H. J., et al. (2015). Alternaria section Alternaria: Species, formae speciales or pathotypes? Studies in Mycology, 82, 1–21. https://doi.org/10.1016/j.simyco.2015.07.001.CrossRefPubMedPubMedCentralGoogle Scholar
- Xu, S., Ni, Z., Ma, L., & Zheng, X. (2017). Control of Alternaria rot of cherry tomatoes by food-grade Laurus nobilis essential oil microemulsion. Journal of Food Safety, 37(1). https://doi.org/10.1111/jfs.12286.