European Journal of Plant Pathology

, Volume 152, Issue 3, pp 783–789 | Cite as

Phenotypic and genotypic description of Puccinia graminis f. sp. tritici race 2SA55 in South Africa

  • W. H. P. BoshoffEmail author
  • Z. A. Pretorius
  • T. G. Terefe
  • C. M. Bender
  • L. Herselman
  • G. J. Maree
  • B. Visser


Race 2SA55 of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici, was described for the first time during 2000 in South Africa. This race is of particular interest as it was the first local report of increased virulence towards barley cultivars by wheat stem rust. Using three original accessions of 2SA55 from the rust collection of the University of the Free State, nine single pustule isolates were established. Phenotyping of isolates to characterise 2SA55 according to the North American nomenclature system revealed variation in virulence for Sr9g on the tester lines Kubanka and Acme, which was not detected in the initial description of 2SA55. Seven isolates coded as BNGSC, which except for virulence on Sr9b resembled the initial avirulence/virulence description for 2SA55. The remaining two isolates with Sr9g virulence coded as BPGSC. The seedling infection types for the two 2SA55 derived races BNGSC and BPGSC on the Stakman differential set as well as on an extended set of Sr lines and 144 South African wheat cultivars and advanced breeding lines revealed no further distinction in reaction between them. Microsatellite analysis indicated that while race BN/PGSC shares phenotypic similarity with several of the South African non-Ug99 races, it was genetically distinct.


Stem rust Puccinia graminis f. sp. tritici Wheat Race description 



The microsatellite analysis was funded by the National Research Foundation, SA (grant number 87692).

Compliance with ethical standards

We hereby declare that our submission is in compliance with the ethical responsibilities and standards of the EJPP as well as those of the University of the Free State.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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ESM 1 (DOC 5230 kb)


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  1. 1.Department of Plant SciencesUniversity of the Free StateBloemfonteinSouth Africa
  2. 2.Agricultural Research Council-Small GrainBethlehemSouth Africa

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