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European Journal of Plant Pathology

, Volume 152, Issue 3, pp 747–757 | Cite as

Assessing synergism of combined applications of Trichoderma harzianum and Pseudomonas fluorescens to control blast and bacterial leaf blight of rice

  • Prashant P. Jambhulkar
  • Pratibha Sharma
  • Raja Manokaran
  • Dilip K. Lakshman
  • Pramod Rokadia
  • Nitiprasad Jambhulkar
Article
  • 73 Downloads

Abstract

Combined application of biocontrol agents (BCAs) have been used to exploit their synergistic potential. But the analysis of additive, synergistic and antagonistic effects of BCAs has rarely been explored for the biocontrol of plant diseases. This study was conducted to evaluate in vitro and in vivo biocontrol potential of the combined application of Trichoderma harzianum strain Th3 and Pseudomonas fluorescens strain RRb11, and the fungicide carbendazim against the rice blast (RB) pathogen, Magnaporthe oryzae, and the bacterial leaf blight (BLB) pathogen, Xanthomonas oryzae pv. oryzae. Combined application of T. harzianum Th3 and P. fluorescens RRb11 synergistically reduced severity of RB by 69.5% in comparison to the untreated control, displaying a synergy factor (SF) of 1.29. P. fluorescens RRb 11 alone and in combination with T. harzianum Th3 and the mixture of T. harzianum Th3 + carbendazim was antagonistic (SF = 0.71 and 0.45, respectively). The combined application of T. harzianum Th3 and P. fluorescens RRb 11 also enhanced several rice plant growth and yield parameters. Additive, synergistic or antagonistic effects among the two BCAs were assessed by applying the numerical hypothesis of ‘Bliss Independence’ which suggested that the combined application of the two BCAs was synergistic in reducing RB but antagonistic with respect to BLB. Out of the six combined application interactions for both the diseases only one interaction (T. harzianum Th3 and P. fluorescens RRb11) was synergistic against RB, which suggested that synergism is a rare event in combined use of BCAs.

Keywords

Trichoderma harzianum Pseudomonas fluorescens Biocontrol Synergism Blast Blight 

Notes

Acknowledgements

The authors are grateful to the Zonal Director of Research, Agricultural Research Station, Banswara, for providing necessary facilities for the experiments.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Involvement of human participants and /or animals

The present research did not involve or practiced on any human or animals.

Informed consent

Authors are ready to provide sufficient detailed information of the present study to the readers for future collaboration and participation in such kind of research with us.

Supplementary material

10658_2018_1519_MOESM1_ESM.doc (72 kb)
ESM 1 (DOC 72 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Prashant P. Jambhulkar
    • 1
  • Pratibha Sharma
    • 2
    • 3
  • Raja Manokaran
    • 2
    • 3
  • Dilip K. Lakshman
    • 4
  • Pramod Rokadia
    • 1
  • Nitiprasad Jambhulkar
    • 5
  1. 1.Agricultural Research Station (MPUAT)BanswaraIndia
  2. 2.Division of Plant PathologyIndian Agricultural Research InstituteNew DelhiIndia
  3. 3.Department of Plant PathologySKNAUJobner-Jaipur (Rajasthan)India
  4. 4.Sustainable Agricultural Systems LaboratoryUSDA-ARSBeltsvilleUSA
  5. 5.ICAR-National Rice Research InstituteCuttackIndia

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