European Journal of Plant Pathology

, Volume 152, Issue 3, pp 735–746 | Cite as

Description and molecular phylogeny of Ditylenchus gilanicus n. sp. (Nematoda: Anguinidae) from northern forests of Iran

  • Ali Yaghoubi
  • Ebrahim PourjamEmail author
  • Weimin Ye
  • Pablo Castillo
  • Majid Pedram


Ditylenchus gilanicus n. sp. is described and illustrated based on morphological, morphometric and molecular characters. The new species is characterized by its small body size, six lines in the lateral field, weak stylet with distinct rounded knobs, median pharyngeal bulb with refractive valvular apparatus, pharyngeal bulb offset from intestine, female reproductive system having long post-vulval uterine sac (PUS), elongate conoid tail with pointed tip and common males with small spicules. Morphological differences between the new species and eight closely related species of the genus namely D. australiae, D. dauniae, D. elegans, D. filenchulus, D. geraerti, D. longicauda, D. medicaginis and D. zeae are discussed. Phylogenetic analyses using partial 18S rDNA and 28S rDNA D2/D3 sequences revealed that the new species belong to D. triformis-group.


18S rDNA 28S rDNA D2/D3 Iran new species phylogeny taxonomy 



We acknowledge the Iranian National Science Foundation (INSF) and Tarbiat Modares University (Iran) for financial supports to this project.

Compliance with ethical standards

The result of this work has not been published previously and is not under consideration elsewhere. All the data are original and the raw data (sequences and measurements as well as microphotographs) are accessible from the part of the journal.


This research was funded by Tarbiat Modares University, Tehran, Iran.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This paper does not describe research on human subjects, therefore ethical approval was not necessary.


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Ali Yaghoubi
    • 1
  • Ebrahim Pourjam
    • 1
    Email author
  • Weimin Ye
    • 2
  • Pablo Castillo
    • 3
  • Majid Pedram
    • 1
  1. 1.Department of Plant Pathology, Faculty of AgricultureTarbiat Modares UniversityTehranIran
  2. 2.Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture and Consumer ServicesRaleighUSA
  3. 3.Instituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC)CórdobaSpain

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