Diaporthe novem isolated from sunflower (Helianthus annuus) and other crop and weed hosts in Australia
- 154 Downloads
In 2011, patches of lodged plants of Helianthus annuus (commercial sunflower) with stem lesions were observed in a commercial crop at Kingsthorpe, Queensland, Australia. Several species of Diaporthe were consistently isolated from the lesions. Diaporthe novem was identified by DNA sequence analysis. In following surveys, Diaporthe novem was found to be associated with other crop species including Cicer arietinum (chickpea), Glycine max (soybean), Lupinus alba (lupin), Sorghum bicolor (sorghum) Vicia faba (faba bean) and Vigna radiata (mungbean), and with the weed species Datura stramonium (common thornapple), Helianthus annuus (wild-type sunflower), Malva parviflora (small flowered mallow), Rapistrum rugosum (turnip weed), Sambucus gaudichaudiana (wild elderberry), Sisymbrium orientale (indian hedge mustard), Sonchus oleraceus (sowthistle), Verbena sp., Vicia sativa (common vetch), and Xanthium strumarium (noogoora burr). In pathogenicity tests, isolates of D. novem from sunflower were highly virulent when re-inoculated on commercial sunflower varieties. This study has identified D. novem as a frequent cause of stem canker of sunflower in the eastern cropping areas of Australia, and extends the known host range of D. novem.
KeywordsChickpea Disease Faba bean Lupin Mungbean Pathogenicity Phomopsis Sorghum Soybean Weed diseases
This work was supported by the Australian Grains Research and Development Corporation under Grants DAQ00154 and DAQ00186 awarded to the Queensland Department of Agriculture and Fisheries, and the University of Southern Queensland. The authors thank Ms. Loretta Serafin (NSW Department of Primary Industries) for assistance with lupin and sunflower samples; Ms. Sara Blake and Ms. Tara Russell (USQ) for technical assistance and Dr. Dean Beasley (Queensland Plant Pathology Herbarium) for advice on specimens.
Sources of funding
This study was funded by the Australian Grains Research and Development Corporation under Grants DAQ00154 and DAQ00186.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Research involving human participants and/or animals
This article does not contain any studies with human participants or animals performed by any of the authors.
- van der Aa, H. A., Noordeloos, M. E., & de Gruyter, J. (1990). Species concepts in some larger genera of the Coelomycetes. Studies in Mycology, 32, 3–19.Google Scholar
- Bureau of Meteorology. (2017a). Climate statistics for Australian locations; Macedon forestry. http://www.bom.gov.au/climate/averages/tables/cw_087036.shtml. Accessed 21 November 2017.
- Bureau of Meteorology. (2017b). Climate statistics for Australian locations; Moree aero. http://www.bom.gov.au/climate/averages/tables/cw_053115.shtml. Accessed 21 November 2017.
- Gao, Y., Liu, F., & Cai, L. (2016). Unravelling Diaporthe species associated with Camellia. Systematics and Biodiversity, 14, 102–117.Google Scholar
- de Hoog, G. S., & Gerrits van den Ende, A. H. (1998). Molecular diagnostics of clinical strains of filamentous basidiomycetes. Mycoses, 41, 183–189.Google Scholar
- International Rules for Seed Testing. (2014). ISTA validated method. Annex to Chapter 7. Seed health testing methods. 7–016. Acidified PDA method for detection of Phomopsis Complex on Soybean. https://www.seedtest.org/upload/cms/user/SH-07-016-2014.pdf. Accessed 21 November 2017.
- Kulik, M.M., & Sinclair, J.B. (1999). Pod and stem blight. In; compendium of soybean diseases, 4th edition. In G.L. Hartman, J.B. Sinclair & J.C. Rupe (Eds) (pp. 32-33). St. Paul: APS press.Google Scholar
- Muntañola-Cvetković, M., Mihaljčević, M., & Petrov, M. (1981). On the identity of the causative agent of a serious Phomopsis-Diaporthe disease in sunflower plants. Nova Hedwigia, 34, 417–435.Google Scholar
- National Climate Centre. (2011a). An extremely wet December leads to widespread flooding across eastern Australia. Special climate statement 24. Melbourne, Vic: Bureau of Meteorology; 15pp.Google Scholar
- National Climate Centre. (2011b). Wettest March on record in Australia. Special Climate Statement 31. Melbourne, Vic: Bureau of Meteorology; 11pp.Google Scholar
- Petrović, K.L., Riccioni, M., Vidić, V., Đorđević, S., Balešević-Tubić, V., Dukić, V., & Miladinov, Z. (2016). First records of Diaporthe novem, D. foeniculina and D. rudis associated with soybean seed decay in Serbia. Plant Disease 100, 2324.Google Scholar
- Thompson, S., Young, A., & Shivas, R. G. (2010). Phomopsis stem canker–an emerging disease of Australian sunflowers. In B. George-Jaeggli & D. J. Jordan (Eds.), Proceedings of the 1st Australian summer grains conference, gold coast, Australia, 21st – 24th June 2010. Barton: Grains Research and Development Corporation.Google Scholar
- Uecker, F. A. (1988). A world list of Phomopsis names with notes on nomenclature, morphology and biology. Mycologia Memoir, 13, 1–231.Google Scholar
- White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR Protocols: A guide to methods and applications (pp. 315–322). San Diego, USA: Academic Press.Google Scholar