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European Journal of Plant Pathology

, Volume 152, Issue 3, pp 723–733 | Cite as

Baseline sensitivity of Phytophthora capsici to the strobilurin fungicide benzothiostrobin and the efficacy of this fungicide

  • Dicheng Ma
  • Jiamei Zhu
  • Leiming He
  • Kaidi Cui
  • Wei Mu
  • Feng Liu
Article
  • 87 Downloads

Abstract

Phytophthora capsici Leonian is a destructive oomycete plant pathogen that causes diseases in a wide range of crops worldwide. Benzothiostrobin is a broad-spectrum strobilurin fungicide that may control pepper Phytophthora blight. In the current study, the sensitivities of 90 P. capsici isolates collected from different regions in southern China to benzothiostrobin were measured. The curative and protective effects of benzothiostrobin against pepper Phytophthora blight were also determined. The formation of sporangia was inhibited by lower concentrations of benzothiostrobin, approximately 1 μg mL−1 on V8 media, than was necessary to inhibit zoospore discharge. The frequency distribution curve for the benzothiostrobin sensitivity was unimodal with mean EC50 values of 1.84 ± 0.24, 0.60 ± 0.10 and 4.44 ± 0.27 for inhibiting mycelial growth, sporangia formation and zoospore discharge, respectively. The P. capsici isolates used in this study exhibited decreased sensitivity to several commonly used effective fungicides, including mefenoxam, cyazofamid, fluazinam and propamocarb. Furthermore, benzothiostrobin had no cross-resistance with azoxystrobin, pyraclostrobin or famoxadone. The disease severity of pepper Phytophthora blight on pepper leaves and plants was dramatically reduced by benzothiostrobin application of 150 μg mL−1. Benzothiostrobin provided both curative and protective properties against pepper Phytophthora blight on detached pepper leaves and potted pepper plants, with greater protective activity than curative activity. These results suggested that benzothiostrobin may be used for the management of P. capsici.

Keywords

Benzothiostrobin Phytophthora capsici Baseline sensitivity Control efficacy 

Notes

Acknowledgements

The National Key Research Development Program of China (2017YFD0201600) has reported this study. We express our thanks to the companies that provided technical-grade fungicide.

Funding

This study was funded by the National Key Research Development Program of China (2017YFD0201600).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10658_2018_1514_MOESM1_ESM.docx (23 kb)
ESM 1 (DOCX 22 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Dicheng Ma
    • 1
  • Jiamei Zhu
    • 1
  • Leiming He
    • 1
  • Kaidi Cui
    • 1
  • Wei Mu
    • 1
  • Feng Liu
    • 1
  1. 1.College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect PestsShandong Agricultural UniversityTai’anPeople’s Republic of China

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