High variability in the N terminus of coat protein among potyviruses and its advantage in producing a specific antibody

  • Dewei Peng
  • Guohua Zheng
  • Zhizhong Zheng
  • Qingxuan Tong
  • Yanlin Ming
Article
  • 19 Downloads

Abstract

Cross reaction often occurs in serological detection for potyviruses that can lead to false positive results. Sequence alignment revealed that the N-terminal sequences of coat proteins (CP) are highly variable among potyviruses. Based on this finding, the preparation of a specific antibody against potyviruses is described here increasing the efficiency of detection and identification. A case study of Turnip mosaic virus (TuMV): a specific fragment “CP-50” (1–50 aa of CP) was obtained by prokaryotic expression and used for producing the polyclonal antibody. Test of specificity indicated that the prepared antibody “Anti-CP-50” strongly reacted with TuMV and is appropriate as a specific anti-TuMV antibody.

Keywords

Potyviruses Turnip mosaic virus Antibody Prokaryotic expression 

Abbreviations

TuMV

Turnip mosaic virus

CP

Coat protein

PAbs

Polyclonal antibodies

MAbs

Monoclonal antibodies

MW

Molecular weight

ELISA

Enzyme linked immunosorbent assay

GRAVY

Grand average of hydropathicity.

Notes

Acknowledgements

The authors thank Prof. Chang Chin-an (Graduate Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Taichung, 41349, Taiwan) for providing experimental materials and technical assistance. We thank Peter Sol Reinach (Wenzhou Medical University) for providing language editing service of this manuscript. This work is supported by the grant from Fujian Provincial Natural Science Foundation of China (2011D002) and Xiamen Municipal Natural Science Foundation (3502Z20127015 & 3502Z20162014).

Author’s contributions

YLM and QXT conceived the experiments, and critically revised the manuscript. GHZ collected the samples. DWP and GHZ performed the experiments. ZZZ analyzed the data. DWP wrote the manuscript.

Compliance with ethical standards

This study was carried out following the guidelines and approval of the Laboratory Animal Ethics Committee of Wenzhou Medical University. The rabbits were anesthetized by 3% pentobarbital sodium (1 ml/kg) when collecting blood samples. After the experiments, rabbits were submitted to euthanasia using anesthetic overdose.

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Dewei Peng
    • 1
    • 2
  • Guohua Zheng
    • 1
  • Zhizhong Zheng
    • 1
  • Qingxuan Tong
    • 1
    • 3
  • Yanlin Ming
    • 1
    • 3
  1. 1.Xiamen Overseas Chinese Subtropical Plant Introduction Garden, National Plant Introduction Quarantine Base, Xiamen Key Laboratory for Plant Introduction, Quarantine and Natural ProductsXiamenChina
  2. 2.School of Ophthalmology and Optometry, Eye HospitalWenzhou Medical UniversityWenzhouChina
  3. 3.College of Chemical EngineeringHuaqiao UniversityXiamenChina

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